{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:50:31Z","timestamp":1791841831284,"version":"4.3.4"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2016,10,28]],"date-time":"2016-10-28T00:00:00Z","timestamp":1477612800000},"content-version":"vor","delay-in-days":138,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Phlebotomus papatasi Scopoli is a medically important insect that has been successfully colonized in the laboratory, and blood feeding is critical for colony propagation. There has been much interest in developing established protocols for in\u00a0vitro blood-feeding systems. The objective of this study was to determine if a Parafilm membrane and a hog\u2019s gut membrane could be successfully used with in\u00a0vitro feeding systems. We evaluated percentages ofP. papatasi females that blood fed on different blood-feeding systems (a mouse, a Hemotek feeder, or a glass feeder) used with either a Parafilm or a hog\u2019s gut membrane, with cohorts of 250 and 500P. papatasi females, and with or without external exhalations. For all feeding system combinations, femaleP. papatasi blood fed in higher percentages when in cohorts of 500 individuals and in the presence of exhalations. Higher percentages ofP. papatasi fed on a mouse, but this study also demonstrates thatP. papatasi will readily feed with in\u00a0vitro feeding systems using a Parafilm membrane or a hog\u2019s gut membrane. This study suggests that femaleP. papatasi may use an invitation effect to blood feed and are attracted to blood sources via chemical olfaction cues, both of which have been characterized in other blood-feeding arthropods. Our study demonstrates that a Parafilm membrane or a hog\u2019s gut membrane, in conjunction with the Hemotek or glass feeder system, is potentially a viable alternative to live rodents to blood feed a colony ofP. papatasi.<\/jats:p>","DOI":"10.1093\/jme\/tjw074","type":"journal-article","created":{"date-parts":[[2016,6,14]],"date-time":"2016-06-14T00:41:05Z","timestamp":1465864865000},"page":"1112-1116","source":"Crossref","is-referenced-by-count":11,"title":["Comparison of In Vivo and In Vitro Methods for Blood Feeding ofPhlebotomus papatasi (Diptera: Psychodidae) in the Laboratory"],"prefix":"10.1093","volume":"53","author":[{"given":"David S.","family":"Denlinger","sequence":"first","affiliation":[{"name":"Department of Biology, Utah State University, Logan, UT 84322 (david.denlinger@aggiemail.usu.edu,j.laine.anderson@gmail.com,scott.bernhardt@usu.edu)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew Y.","family":"Li","sequence":"first","affiliation":[{"name":"USDA, ARS, Knipling-Bushland U.S. Livestock Insect Research Laboratory, Kerrville, TX 78028 (Andrew.Li@ars.usda.gov)"},{"name":"Current address: USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, MD 20705"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan L.","family":"Durham","sequence":"first","affiliation":[{"name":"Ecology Center, Utah State University, Logan, UT 84322 (susan.durham@usu.edu)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip G.","family":"Lawyer","sequence":"first","affiliation":[{"name":"Laboratory of Parasitic Diseases, Intracellular Parasite Biology Section, National Institutes of Health, Bethesda, MD 20892 (plawyer349@verizon.net)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph L.","family":"Anderson","sequence":"first","affiliation":[{"name":"Department of Biology, Utah State University, Logan, UT 84322 (david.denlinger@aggiemail.usu.edu,j.laine.anderson@gmail.com,scott.bernhardt@usu.edu)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Bernhardt","sequence":"first","affiliation":[{"name":"Department of Biology, Utah State University, Logan, UT 84322 (david.denlinger@aggiemail.usu.edu,j.laine.anderson@gmail.com,scott.bernhardt@usu.edu)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,6,12]]},"reference":[{"key":"2020060116395948000_tjw074-B1","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1590\/S0074-02762011000600013","article-title":"How to increase the population of aPhlebotomus perniciosus (Diptera: Psychodidae) colony: A new method","volume":"106","author":"Alarc\u00f3n-Elbal","year":"2011","journal-title":"Mem. 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