{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T22:33:02Z","timestamp":1778279582043,"version":"3.51.4"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T00:00:00Z","timestamp":1567036800000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/18046-7"],"award-info":[{"award-number":["2013\/18046-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/27078-2"],"award-info":[{"award-number":["2014\/27078-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/23176-0"],"award-info":[{"award-number":["2014\/23176-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["308632\/2018-4"],"award-info":[{"award-number":["308632\/2018-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["305872\/2013-3"],"award-info":[{"award-number":["305872\/2013-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The bacterium Rickettsia rickettsii is the agent of Brazilian spotted fever (BSF), a highly fatal disease that is transmitted in Brazil mainly by the tick Amblyomma sculptum Berlese, which uses capybaras (Hydrochoerus hydrochaeris Linnaeus) as major hosts. In 2015, we captured nine capybaras in a BSF-endemic area of southeastern Brazil. From each capybara, we collected blood sera that were tested through the immunofluorescence assay using Rickettsia spp. antigens, and A. sculptum ticks, processed for isolation of R. rickettsii through guinea pig inoculation. All capybaras (100%) were seroreactive to Rickettsia spp., with highest titers to R. rickettsii. A total of 166 A. sculptum ticks were macerated and inoculated into nine guinea pigs, from which only one presented high fever and seroconverted to R. rickettsii. Blood from this febrile animal was inoculated into additional guinea pigs (guinea pig passages), which also became febrile and seroconverted, or when euthanized during the fever period, their internal organs (spleen, lung) were shown to contain R. rickettsii DNA. The present rickettsial isolate has been maintained cryopreserved as infected guinea pig organs. There was at least one R. rickettsii-infected tick among the 166 macerated ticks, giving a minimal infection rate of 0.6% (1\/166). This infection rate is within the range of previous studies, which reported that only 0.05% to at most 1.28% A. sculptum ticks were infected by R. rickettsii in other BSF-endemic areas. These low infection rates support the low incidence of BSF, despite of A. sculptum being the most frequent human-biting tick in southeastern Brazil.<\/jats:p>","DOI":"10.1093\/jme\/tjz141","type":"journal-article","created":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T06:17:04Z","timestamp":1567059424000},"page":"308-311","source":"Crossref","is-referenced-by-count":34,"title":["<i>Rickettsia rickettsii<\/i>\n                    (Rickettsiales: Rickettsiaceae) Infecting\n                    <i>Amblyomma sculptum<\/i>\n                    (Acari: Ixodidae) Ticks and Capybaras in a Brazilian Spotted Fever-Endemic Area of Brazil"],"prefix":"10.1093","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6923-7183","authenticated-orcid":false,"given":"Francisco B","family":"Costa","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo , S\u00e3o Paulo, SP, Brazil"},{"name":"Departamento de Patologia, Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancia Animal, Universidade Estadual do Maranh\u00e3o , S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monize","family":"Gerardi","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo , S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina de C","family":"Binder","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo , S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hector R","family":"Benatti","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo , S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Carolina de Azevedo","family":"Serpa","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo , S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Lopes","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Florestais, Universidade de S\u00e3o Paulo, Escola Superior de Agricultura \u2018Luiz de Queiroz\u2019 , Piracicaba, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermes R","family":"Luz","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo , S\u00e3o Paulo, SP, Brazil"},{"name":"Departamento de Patologia, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia do RENORBIO, Ponto Focal Maranh\u00e3o, Universidade Federal do Maranh\u00e3o , S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katia M P M B","family":"Ferraz","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Florestais, Universidade de S\u00e3o Paulo, Escola Superior de Agricultura \u2018Luiz de Queiroz\u2019 , Piracicaba, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo B","family":"Labruna","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo , S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,8,28]]},"reference":[{"key":"2026041420164061400_CIT0001","doi-asserted-by":"crossref","first-page":"653","DOI":"10.3389\/fphys.2019.00653","article-title":"Comparative susceptibility of different populations of Amblyomma sculptum to Rickettsia rickettsii","volume":"10","author":"Gerardi","year":"2019","journal-title":"Front. Physiol"},{"key":"2026041420164061400_CIT0002","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1590\/S0074-02762005000800004","article-title":"Detection of Rickettsia rickettsii in the tick Amblyomma cajennense in a new Brazilian spotted fever-endemic area in the state of Minas Gerais","volume":"100","author":"Guedes","year":"2005","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"2026041420164061400_CIT0003","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1590\/S1984-29612011000400009","article-title":"Rickettsia species infecting Amblyomma ticks from an area endemic for Brazilian spotted fever in Brazil","volume":"20","author":"Guedes","year":"2011","journal-title":"Rev. Bras. Parasitol. Vet"},{"key":"2026041420164061400_CIT0004","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s10493-006-9027-0","article-title":"Ticks (Ixodidae) on humans in South America","volume":"40","author":"Guglielmone","year":"2006","journal-title":"Exp. Appl. Acarol"},{"key":"2026041420164061400_CIT0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-7-7","article-title":"Rickettsial infection in Amblyomma cajennense ticks and capybaras (Hydrochoerus hydrochaeris) in a Brazilian spotted fever-endemic area","volume":"7","author":"Krawczak","year":"2014","journal-title":"Parasit. Vectors"},{"key":"2026041420164061400_CIT0006","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/j.1749-6632.2009.04516.x","article-title":"Ecology of rickettsia in South America","volume":"1166","author":"Labruna","year":"2009","journal-title":"Ann. NY Acad. 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Entomol"},{"key":"2026041420164061400_CIT0025","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.vetpar.2008.12.010","article-title":"Experimental infection of capybaras Hydrochoerus hydrochaeris by Rickettsia rickettsii and evaluation of the transmission of the infection to ticks Amblyomma cajennense","volume":"161","author":"Souza","year":"2009","journal-title":"Vet. Parasitol"},{"key":"2026041420164061400_CIT0026","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1590\/S1519-69842006000200019","article-title":"Capybaras in an anthropogenic habitat in Southeastern Brazil","volume":"66","author":"Verdade","year":"2006","journal-title":"Braz. J. 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