{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:55:29Z","timestamp":1779332129582,"version":"3.51.4"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T00:00:00Z","timestamp":1569283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"IEC and by the Programa de Apoio \u00e0 Publica\u00e7\u00e3o Qualificada","award":["01\/2019 PROPESP\/UFPA"],"award-info":[{"award-number":["01\/2019 PROPESP\/UFPA"]}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior Brasil","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In urban ecotourism parks, the life cycle of American cutaneous leishmaniasis (ACL) agents can remain established, where phlebotomines may comprise potential risks for visitors. The present study aimed to survey the phlebotomine fauna of a forest park \u2018Bosque Rodriques Alves-Jardim Bot\u00e2nico da Amaz\u00f4nia\u2019 (BRAJBA), in the urban area of Bel\u00e9m, Brazil. The park was monthly surveyed in 2018 using CDC light traps placed in ground and canopy strata. Leishmania spp. isolated from dissected females were characterized by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) analysis. Fluctuations in specimen capture were correlated with rainfall. Nyssomyia antunesi (Coutinho, 1939) was predominant for all surveyed ecotopes and capture methods in both areas. Females of Ny. antunesi resting on tree bases were observed attempting to bite researchers during early morning. One Bichromomyia flaviscutellata (Mangabeira, 1942) and one Trichophoromyia brachipyga (Mangabeira, 1942) were found naturally infected by flagellates. Only the strain from Th. brachipyga was isolated and characterized as Leishmania (Viannia) lainsoni Silveira, Shaw, Braga and Ishikawa, 1987. Monthly fluctuations of the three most abundant species, Ny. antunesi, Trichophoromyia ubiquitalis (Mangabeira, 1942) and Th. brachypiga, had statistically significant negative correlations with rainfall. The present study provided further information to better understand ACL ecology in the Bel\u00e9m urban area, where the urban parks surveyed appeared to offer potential risk of contracting the disease, thus requiring environmental management. These observations highlighted the need for including Ny. antunesi, Bi. flaviscutellata, Th. ubiquitalis, and Th. brachypiga in the priority list for continuous entomological surveillance.<\/jats:p>","DOI":"10.1093\/jme\/tjz153","type":"journal-article","created":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T14:21:35Z","timestamp":1569334895000},"page":"281-288","source":"Crossref","is-referenced-by-count":21,"title":["Phlebotomines (Diptera: Psychodidae) from a Urban Park of Bel\u00e9m, Par\u00e1 State, Northern Brazil and Potential Implications in the Transmission of American Cutaneous Leishmaniasis"],"prefix":"10.1093","volume":"57","author":[{"given":"Yetsenia D V","family":"S\u00e1nchez Uzc\u00e1tegui","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Biologia de Agentes Infecciosos e Parasit\u00e1rios, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m, Par\u00e1 State, Brazil"},{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Ananindeua, Par\u00e1 State, Brazil"},{"name":"Departamento de Biolog\u00eda, Faculdad de Ciencias, Universidad de Los Andes, M\u00e9rida, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0229-4861","authenticated-orcid":false,"given":"Thiago","family":"Vasconcelos Dos Santos","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Ananindeua, Par\u00e1 State, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0412-6060","authenticated-orcid":false,"given":"Fernando T","family":"Silveira","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Ananindeua, Par\u00e1 State, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia K S","family":"Ramos","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Ananindeua, Par\u00e1 State, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo Jos\u00e9 M","family":"Dos Santos","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Biologia de Agentes Infecciosos e Parasit\u00e1rios, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m, Par\u00e1 State, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marinete M","family":"P\u00f3voa","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Biologia de Agentes Infecciosos e Parasit\u00e1rios, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m, Par\u00e1 State, Brazil"},{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Ananindeua, Par\u00e1 State, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,9,24]]},"reference":[{"key":"2020010907383233000_CIT0001","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1186\/1756-3305-7-360","article-title":"Sand fly fauna (Diptera: Psychodidade: Phlebotominae) in an area of leishmaniasis transmission in the municipality of Rio Branco, sate of Acre","volume":"7","author":"Araujo-Pereira","year":"2014","journal-title":"Brazil. Parasit Vectors"},{"key":"2020010907383233000_CIT0002","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1590\/S0074-02762008000800003","article-title":"Studies on the sandfly fauna (Diptera: Psychodidae: Phlebotominae) from transmission areas of American Cutaneous Leishmaniasis in state of Acre, Brazil","volume":"103","author":"Azevedo","year":"2008","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"2020010907383233000_CIT0003","volume-title":"Minist\u00e9rio da sa\u00fade. Secretaria de vigil\u00e2ncia em sa\u00fade. Departamento de vigil\u00e2ncia das doen\u00e7as transmiss\u00edveis. Manual de vigil\u00e2ncia da leishmaniose tegumentar","author":"Brasil","year":"2017","edition":"2nd ed"},{"key":"2020010907383233000_CIT0004","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1051\/parasite\/2019030","article-title":"First report of Leishmania (Viannia) lindenbergi causing tegumentary leishmaniasis in the Brazilian western Amazon region","volume":"26","author":"Cantanh\u00eade","year":"2019","journal-title":"Parasite"},{"key":"2020010907383233000_CIT0005","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1093\/jmedent\/8.4.415","article-title":"Daily and seasonal man-biting activity of phlebotomine sandflies in Panama","volume":"8","author":"Chaniotis","year":"1971","journal-title":"J. Med. Entomol"},{"key":"2020010907383233000_CIT0006","doi-asserted-by":"crossref","first-page":"29","DOI":"10.5123\/S2176-62232014000200004","article-title":"Phlebotomine sand flies (Diptera: Psychodidae) in forest fragments of Bel\u00e9m metropolitan area, Par\u00e1 State, Brazil, with considerations on vectors of American cutaneous leishmaniasis agents","volume":"5","author":"Ferreira","year":"2014","journal-title":"Rev Pan-Amazonica Saude"},{"key":"2020010907383233000_CIT0007","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1590\/S0074-02762002000100008","article-title":"Species diversity and flagellate infections in the sand fly fauna near Porto Grande, State of Amap\u00e1, Brazil (Diptera: Psychodidae. Kinetoplastida: Trypanosomatidae)","volume":"97","author":"Freitas","year":"2002","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"2020010907383233000_CIT0008","doi-asserted-by":"crossref","first-page":"9819723","DOI":"10.1155\/2016\/9819723","article-title":"Phlebotomines (Diptera: Psychodidae) in a hydroelectric system affected area from Northern Amazonian Brazil: further insights into the effects of environmental changes on vector ecology","volume":"2016","author":"Furtado","year":"2016","journal-title":"J. Trop. Med"},{"key":"2020010907383233000_CIT0009","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/978-3-319-75544-1_2","article-title":"Phlebotominae (Diptera, Psychodidae): classification, morphology, and terminology of adults and identification of American taxa,","volume-title":"Brazilian sand flies: biology, taxonomy, medical importance and control","author":"Galati","year":"2018"},{"key":"2020010907383233000_CIT0010","first-page":"1","article-title":"PAST: paleontological statistics software package for education and data analysis","volume":"4","author":"Hammer","year":"2001","journal-title":"Paleontol Electro"},{"key":"2020010907383233000_CIT0011","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/0035-9203(68)90090-4","article-title":"Leishmaniasis in Brazil: I. Observations on enzootic rodent leishmaniasis\u2013incrimination of Lutzomyia flaviscutellata (Mangabeira) as the vector in the Lower Amazonian Basin","volume":"62","author":"Lainson","year":"1968","journal-title":"Trans. R. Soc. Trop. Med. Hyg"},{"key":"2020010907383233000_CIT0012","first-page":"1","article-title":"Evolution, classification and geographical distribution,","volume-title":"The leishmaniases in biology and medicine","author":"Lainson","year":"1987"},{"key":"2020010907383233000_CIT0013","doi-asserted-by":"crossref","DOI":"10.1002\/9780470688618.taw0182","article-title":"New world Leishmaniasis","volume-title":"Topley & Wilson\u2019s microbiology and microbial infections","author":"Lainson","year":"2010"},{"key":"2020010907383233000_CIT0014","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1590\/S0074-02761992000300016","article-title":"Further observations on Lutzomyia ubiquitalis (Psychodidae: Phlebotominae), the sandfly vector of Leishmania (Viannia) lainsoni","volume":"87","author":"Lainson","year":"1992","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"2020010907383233000_CIT0015","first-page":"359","article-title":"La leishmaniose en Guyane Fran\u00e7aise. I. \u00c9tude de l\u2019\u00e9cologie et du taux d\u2019infection naturelle d\u2019un vecteur Lutzomyia (Nyssomyia) umbratilis Ward et Fraiha, 1977 en saison s\u00e8che. Considerations epid\u00e9miologiques","volume":"18","author":"Le Pont","year":"1980","journal-title":"Cahiers ORSTOM Ser Entomol Med Parasitol"},{"key":"2020010907383233000_CIT0016","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1590\/S0102-311X2007000100010","article-title":"Padroniza\u00e7\u00e3o de condi\u00e7\u00f5es para detec\u00e7\u00e3o de DNA de Leishmania spp. em flebotom\u00edneos (Diptera, Psychodidae) pela rea\u00e7\u00e3o em cadeia da polimerase","volume":"23","author":"Paiva","year":"2007","journal-title":"Cad Saude Publica"},{"key":"2020010907383233000_CIT0017","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1146\/annurev-ento-120811-153557","article-title":"Biology of phlebotomine sand flies as vectors of disease agents","volume":"58","author":"Ready","year":"2013","journal-title":"Annu Rev Entomol"},{"key":"2020010907383233000_CIT0018","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1017\/S0007485300015248","article-title":"The ecology of Lutzomyia umbratilis Ward & Fraiha (Diptera: Psychodidae), the major vector to man of Leishmania braziliensis guyanensis in north-eastern Amazonian Brazil","volume":"76","author":"Ready","year":"1986","journal-title":"Bull. Entomol. Res"},{"key":"2020010907383233000_CIT0019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S0034-89102009005000072","article-title":"Distribui\u00e7\u00e3o de Lutzomyia whitmani em fitorregi\u00f5es do estado do Maranh\u00e3o, Brasil","volume":"43","author":"Reb\u00ealo","year":"2009","journal-title":"Rev Saude Publica"},{"key":"2020010907383233000_CIT0020","volume-title":"Fleb\u00f3tomos do estado do Par\u00e1","author":"Ryan","year":"1986"},{"key":"2020010907383233000_CIT0021","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/0035-9203(87)90133-7","article-title":"Leishmaniasis in Brazil. XXIV. Natural flagellate infections of sandflies (Diptera: Psychodidae) in Par\u00e1 State, with particular reference to the r\u00f4le of Psychodopygus wellcomei as the vector of Leishmania braziliensis braziliensis in the Serra dos Caraj\u00e1s","volume":"81","author":"Ryan","year":"1987","journal-title":"Trans. R. Soc. Trop. Med. Hyg"},{"key":"2020010907383233000_CIT0022","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1590\/S0074-02761991000100021","article-title":"Cutaneous leishmaniasis in the Amazon region: natural infection of the sandfly Lutzomyia ubiquitalis (Psychodidae: Phlebotominae) by Leishmania (Viannia) Lainsoni in Par\u00e1 State, Brazil","volume":"86","author":"Silveira","year":"1991","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"2020010907383233000_CIT0023","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1051\/parasite\/200209143","article-title":"An outbreak of cutaneous leishmaniasis among soldiers in Bel\u00e9m, Par\u00e1 State, Brazil, caused by Leishmania (Viannia) lindenbergi n. sp. A new leishmanial parasite of man in the Amazon region","volume":"9","author":"Silveira","year":"2002","journal-title":"Parasite"},{"key":"2020010907383233000_CIT0024","first-page":"1203","article-title":"Leishmaniose Tegumentar Americana,","volume-title":"Medicina tropical e infectologia na Amaz\u00f4nia","author":"Silveira","year":"2013"},{"key":"2020010907383233000_CIT0025","first-page":"1691","article-title":"Leishmaniose Tegumentar Americana,","volume-title":"Tratado de Dermatologia","author":"Silveira","year":"2018","edition":"3rd ed"},{"key":"2020010907383233000_CIT0026","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.diagmicrobio.2009.08.013","article-title":"Leishmania spp. identification by polymerase chain reaction-restriction fragment length polymorphism analysis and its applications in French Guiana","volume":"66","author":"Simon","year":"2010","journal-title":"Diagn. Microbiol. Infect. Dis"},{"key":"2020010907383233000_CIT0027","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1590\/0074-02760170024","article-title":"Trichophoromyia auraensis is a putative vector","volume":"112","author":"Teles","year":"2017","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"2020010907383233000_CIT0028","doi-asserted-by":"crossref","first-page":"511","DOI":"10.4269\/ajtmh.2012.11-0708","article-title":"Natural Leishmania infection of Lutzomyia auraensis in Madre de Dios, Peru, detected by a fluorescence resonance energy transfer-based real-time polymerase chain reaction","volume":"87","author":"Valdivia","year":"2012","journal-title":"Am. J. Trop. Med. Hyg"},{"key":"2020010907383233000_CIT0029","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1186\/s13071-018-3190-0","article-title":"Ecological aspects of Phlebotomines (Diptera: Psychodidae) and the transmission of American cutaneous leishmaniasis agents in an Amazonian\/ Guianan bordering area","volume":"11","author":"Vasconcelos dos Santos","year":"2018","journal-title":"Parasit. Vectors"},{"key":"2020010907383233000_CIT0030","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1093\/jme\/tjy194","article-title":"Trichophoromyia iorlandobaratai (Diptera: Psychodidae), a new phlebotomine species from the Brazilian Amazonia","volume":"56","author":"Vasconcelos dos Santos","year":"2019","journal-title":"J. Med. Entomol"},{"key":"2020010907383233000_CIT0031","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1590\/S0074-0276108042013011","article-title":"Seasonal variation and natural infection of Lutzomyia antunesi (Diptera: Psychodidae: Phlebotominae), an endemic species in the Orinoquia region of Colombia","volume":"108","author":"V\u00e1squez-Trujillo","year":"2013","journal-title":"Mem Inst Oswaldo Cruz"}],"container-title":["Journal of Medical Entomology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jme\/article-pdf\/57\/1\/281\/31775964\/tjz153.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jme\/article-pdf\/57\/1\/281\/31775964\/tjz153.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T07:39:55Z","timestamp":1578555595000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jme\/article\/57\/1\/281\/5573326"}},"subtitle":[],"editor":[{"given":"Lane","family":"Foil","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,9,24]]},"references-count":31,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,9,24]]},"published-print":{"date-parts":[[2020,1,9]]}},"URL":"https:\/\/doi.org\/10.1093\/jme\/tjz153","relation":{},"ISSN":["0022-2585","1938-2928"],"issn-type":[{"value":"0022-2585","type":"print"},{"value":"1938-2928","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,1]]},"published":{"date-parts":[[2019,9,24]]}}}