{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T19:46:29Z","timestamp":1786304789925,"version":"build-2736575974"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100015304","name":"Research Services Council, University of Nebraska Kearney","doi-asserted-by":"publisher","award":["GM103427"],"award-info":[{"award-number":["GM103427"]}],"id":[{"id":"10.13039\/100015304","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["5P20GM103427"],"award-info":[{"award-number":["5P20GM103427"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Dermacentor variabilis is the predominant tick species in Nebraska and is presumed to be the primary vector of Rickettsia rickettsii associated with cases of Rocky Mountain spotted fever (RMSF). Interestingly, RMSF cases in Nebraska have increased on a year-to-year basis, yet the prevalence of R. rickettsii in D. variabilis ticks has not been established for Nebraska. Here we sought to set a baseline for the prevalence of R. rickettsii and other spotted fever group (SFG) rickettsiae harbored by D. variabilis ticks. Over a 3-yr period, D. variabilis were collected along the Platte River in south central Nebraska. Individual tick DNA was analyzed using endpoint PCR to identify ticks carrying SFG rickettsiae. In total, 927 D. variabilis were analyzed by PCR and 38 (4.1%) ticks tested positive for SFG rickettsiae. Presumptive positives were sequenced to identify the Rickettsia species, of which 29 (76%) were R. montanensis, 5 (13%) were R. amblyommatis, 4 (11%) were R. bellii, and R. rickettsii was not detected. These data indicate that R. rickettsii is likely at a low prevalence in south central Nebraska and spillover of R. amblyommatis into D. variabilis is likely occurring due to the invasive lone star tick (Amblyomma americanum). In addition, our data suggest that R. montanensis and R. amblyommatis could be associated with the increase in SFG rickettsiae infections in Nebraska. This information will be of value to clinicians and the general public for evaluating diagnosis of disease- and risk-associated environmental exposure, respectively.<\/jats:p>","DOI":"10.1093\/jme\/tjz167","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T05:21:57Z","timestamp":1569993717000},"page":"519-523","source":"Crossref","is-referenced-by-count":6,"title":["Molecular Detection of Spotted Fever Group Rickettsiae (Rickettsiales: Rickettsiaceae) in Dermacentor variabilis (Acari: Ixodidae) Collected Along the Platte River in South Central Nebraska"],"prefix":"10.1093","volume":"57","author":[{"given":"Brandon E","family":"Luedtke","sequence":"first","affiliation":[{"name":"Biology Department, University of Nebraska at Kearney, Kearney, NE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie J","family":"Shaffer","sequence":"additional","affiliation":[{"name":"Biology Department, University of Nebraska at Kearney, Kearney, NE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estrella","family":"Monrroy","sequence":"additional","affiliation":[{"name":"Biology Department, University of Nebraska at Kearney, Kearney, NE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey W","family":"Willicott","sequence":"additional","affiliation":[{"name":"Biology Department, University of Nebraska at Kearney, Kearney, NE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4154-929X","authenticated-orcid":false,"given":"Travis J","family":"Bourret","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, NE"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,10,2]]},"reference":[{"key":"2020110419123073800_CIT0001","first-page":"1","article-title":"Summary of notifiable diseases\u2013United States, 2012","volume":"61","author":"Adams","year":"2014","journal-title":"MMWR. 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Rep"},{"key":"2020110419123073800_CIT0005","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1089\/vbz.2007.0271","article-title":"Tick-borne diseases in North Carolina: is \u201cRickettsia amblyommii\u201d a possible cause of rickettsiosis reported as Rocky Mountain spotted fever?","volume":"8","author":"Apperson","year":"2008","journal-title":"Vector Borne Zoonotic Dis"},{"key":"2020110419123073800_CIT0006","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1089\/vbz.2013.1325","article-title":"\u201cRickettsia amblyommii\u201d and R. montanensis infection in dogs following natural exposure to ticks","volume":"14","author":"Barrett","year":"2014","journal-title":"Vector Borne Zoonotic Dis"},{"key":"2020110419123073800_CIT0007","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.rr6502a1","article-title":"Diagnosis and management of tickborne rickettsial diseases: rocky mountain spotted fever and other spotted fever group rickettsioses, ehrlichioses, and anaplasmosis - United States","volume":"65","author":"Biggs","year":"2016","journal-title":"MMWR. Recomm. Rep"},{"key":"2020110419123073800_CIT0008","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1089\/vbz.2007.0121","article-title":"Detection of Rickettsia amblyommii in association with a tick bite rash","volume":"7","author":"Billeter","year":"2007","journal-title":"Vector Borne Zoonotic Dis"},{"key":"2020110419123073800_CIT0009","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.idc.2018.10.010","article-title":"The rickettsioses: a practical update","volume":"33","author":"Blanton","year":"2019","journal-title":"Infect. Dis. Clin. North Am"},{"key":"2020110419123073800_CIT0010","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1093\/jmedent\/12.3.269","article-title":"A review of Rocky Mountain spotted fever (tick-borne typhus), its agent, and its tick vectors in the United States","volume":"12","author":"Burgdorfer","year":"1975","journal-title":"J. Med. 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Entomol"},{"key":"2020110419123073800_CIT0030","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1089\/vbz.2015.1856","article-title":"Prevalence, distribution, and development of an ecological niche model of dermacentor variabilis ticks positive for Rickettsia montanensis","volume":"16","author":"St John","year":"2016","journal-title":"Vector Borne Zoonotic Dis"},{"key":"2020110419123073800_CIT0031","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1089\/vbz.2010.0099","article-title":"Infrequency of Rickettsia rickettsii in Dermacentor variabilis removed from humans, with comments on the role of other human-biting ticks associated with spotted fever group Rickettsiae in the United States","volume":"11","author":"Stromdahl","year":"2011","journal-title":"Vector Borne Zoonotic Dis"},{"key":"2020110419123073800_CIT0032","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1086\/592254","article-title":"Rickettsia parkeri Rickettsiosis and Its Clinical Distinction from Rocky Mountain Spotted Fever","volume":"47","author":"Sumner","year":"2008","journal-title":"Clin. Infect. Dis"},{"key":"2020110419123073800_CIT0033","first-page":"1284","article-title":"Prevalence of rickettsia species (Rickettsiales: Rickettsiaceae) in Dermacentor variabilis Ticks (Acari: Ixodidae) in North Carolina","volume":"55","author":"Sutton","year":"2018","journal-title":"J. Med. Entomol"},{"key":"2020110419123073800_CIT0034","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1603\/ME10130","article-title":"Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) in Amblyomma americanum (Acari: Ixodidae) From Kansas","volume":"48","author":"Telford","year":"2011","journal-title":"J. Med. Entomol"},{"key":"2020110419123073800_CIT0035","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1093\/jme\/tjv086","article-title":"Rickettsia parkeri transmission to Amblyomma americanum by cofeeding with Amblyomma maculatum (Acari: Ixodidae) and potential for spillover","volume":"52","author":"Wright","year":"2015","journal-title":"J. Med. Entomol"}],"container-title":["Journal of Medical Entomology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jme\/article-pdf\/57\/2\/519\/33829104\/tjz167.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jme\/article-pdf\/57\/2\/519\/33829104\/tjz167.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T00:57:57Z","timestamp":1604537877000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jme\/article\/57\/2\/519\/5580100"}},"subtitle":[],"editor":[{"given":"Kevin","family":"Macaluso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,10,2]]},"references-count":35,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,10,2]]},"published-print":{"date-parts":[[2020,2,27]]}},"URL":"https:\/\/doi.org\/10.1093\/jme\/tjz167","relation":{},"ISSN":["0022-2585","1938-2928"],"issn-type":[{"value":"0022-2585","type":"print"},{"value":"1938-2928","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,3]]},"published":{"date-parts":[[2019,10,2]]}}}