{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:14:39Z","timestamp":1789427679817,"version":"build-2803163510"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T00:00:00Z","timestamp":1594339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900747"],"award-info":[{"award-number":["81900747"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chinese Academy of Medical Science Initiative for Innovative Medicine","award":["2016-I2M-3-006"],"award-info":[{"award-number":["2016-I2M-3-006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,12,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pancreatic islet microcirculation, consisting of pancreatic islet microvascular endothelial cells (IMECs) and pericytes (IMPCs), provides crucial support for the physiological function of pancreatic islet. Emerging evidence suggests that pancreatic islet microcirculation is impaired in type 1 diabetes mellitus (T1DM). Here, we investigated the potential ultrastructural protective effects of insulin against streptozotocin (STZ)-induced ultrastructural abnormalities of the pancreatic islet microcirculation in T1DM mouse model. For this purpose, pancreatic tissues were collected from control, STZ-induced T1DM and insulin-treated mice, and a pancreatic IMECs cell line (MS1) was cultured under control, 35\u00a0mM glucose with or without 10\u22128\u00a0M insulin conditions. Transmission and scanning electron microscopies were employed to evaluate the ultrastructure of the pancreatic islet microcirculation. We observed ultrastructural damage to IMECs and IMPCs in the type 1 diabetic group, as demonstrated by destruction of the cytoplasmic membrane and organelles (mainly mitochondria), and this damage was substantially reversed by insulin treatment. Furthermore, insulin inhibited collagenous fiber proliferation and alleviated edema of the widened pancreatic islet exocrine interface in T1DM mice. We conclude that insulin protects against T1DM-induced ultrastructural abnormalities of the pancreatic islet microcirculation.<\/jats:p>","DOI":"10.1093\/jmicro\/dfaa036","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T19:10:54Z","timestamp":1594235454000},"page":"381-390","source":"Crossref","is-referenced-by-count":4,"title":["Insulin protects against type 1 diabetes mellitus-induced ultrastructural abnormalities of pancreatic islet microcirculation"],"prefix":"10.1093","volume":"69","author":[{"given":"Bing","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Electron Microscopy, Pathology Center, Peking University First Hospital, Beijing, 100034, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6750-5068","authenticated-orcid":false,"given":"Mingming","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China"},{"name":"Diabetes Research Center, Chinese Academy of Medical Science, Beijing 100005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China"},{"name":"Diabetes Research Center, Chinese Academy of Medical Science, Beijing 100005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailing","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honggang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijuan","family":"Xiu","sequence":"additional","affiliation":[{"name":"Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,7,10]]},"reference":[{"key":"2020120306470583700_ref1","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1001\/jama.2013.168118","article-title":"Prevalence and control of diabetes in Chinese adults","volume":"310","author":"Xu","year":"2013","journal-title":"JAMA"},{"key":"2020120306470583700_ref2","doi-asserted-by":"crossref","first-page":"j5295","DOI":"10.1136\/bmj.j5295","article-title":"Incidence of type 1 diabetes in China, 2010-13: population based study","volume":"360","author":"Weng","year":"2018","journal-title":"BMJ"},{"key":"2020120306470583700_ref3","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1177\/1479164113484165","article-title":"Assessment of skeletal muscle microcirculation in type 2 diabetes mellitus using dynamic contrast-enhanced ultrasound: a pilot study","volume":"10","author":"Amarteifio","year":"2013","journal-title":"Diab. Vasc. Dis. Res."},{"key":"2020120306470583700_ref4","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/1475-2840-12-115","article-title":"Impaired skin microcirculation in paediatric patients with type 1 diabetes mellitus","volume":"12","author":"Heimhalt-El Hamriti","year":"2013","journal-title":"Cardiovasc. Diabetol."},{"key":"2020120306470583700_ref5","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1080\/10739680701285617","article-title":"Microcirculation and the metabolic syndrome","volume":"14","author":"Wiernsperger","year":"2007","journal-title":"Microcirculation"},{"key":"2020120306470583700_ref6","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1007\/s00268-006-0719-8","article-title":"Islet vasculature as a regulator of endocrine pancreas function","volume":"31","author":"Ballian","year":"2007","journal-title":"World J. Surg."},{"key":"2020120306470583700_ref7","doi-asserted-by":"crossref","first-page":"4154","DOI":"10.2337\/db13-0071","article-title":"Vascular endothelial growth factor-a and islet vascularization are necessary in developing, but not adult, pancreatic islets","volume":"62","author":"Reinert","year":"2013","journal-title":"Diabetes"},{"key":"2020120306470583700_ref8","doi-asserted-by":"crossref","first-page":"3008","DOI":"10.2337\/db16-0365","article-title":"Islet pericytes are required for beta-cell maturity","volume":"65","author":"Sasson","year":"2016","journal-title":"Diabetes"},{"key":"2020120306470583700_ref9","doi-asserted-by":"crossref","first-page":"e43591","DOI":"10.1371\/journal.pone.0043591","article-title":"KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation in vitro and in vivo","volume":"7","author":"Kim","year":"2012","journal-title":"PLoS One"},{"key":"2020120306470583700_ref10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s00441-003-0745-x","article-title":"Endothelial-pericyte interactions in angiogenesis","volume":"314","author":"Gerhardt","year":"2003","journal-title":"Cell Tissue Res."},{"key":"2020120306470583700_ref11","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1111\/1753-0407.12516","article-title":"Insulin treatment restores islet microvascular vasomotion function in diabetic mice","volume":"9","author":"Liu","year":"2017","journal-title":"J. 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Med."},{"key":"2020120306470583700_ref15","doi-asserted-by":"crossref","first-page":"e2894","DOI":"10.1002\/dmrr.2894","article-title":"Ultrastructural alterations of pancreatic beta cells in human diabetes mellitus","volume":"33","author":"Masini","year":"2017","journal-title":"Diabetes Metab. Res."},{"key":"2020120306470583700_ref16","doi-asserted-by":"crossref","first-page":"5200","DOI":"10.1210\/en.2012-1490","article-title":"Prevention of diabetes in db\/db mice by dietary soy is independent of isoflavone levels","volume":"153","author":"Zimmermann","year":"2012","journal-title":"Endocrinology"},{"key":"2020120306470583700_ref17","first-page":"725","article-title":"Ultrastructural islet study of early fibrosis in the Ren2 rat model of hypertension. 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Immunol."},{"key":"2020120306470583700_ref20","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1161\/ATVBAHA.107.161521","article-title":"Endothelial-mural cell signaling in vascular development and angiogenesis","volume":"29","author":"Gaengel","year":"2009","journal-title":"Arterioscler. Thromb. Vasc. 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Redox Signal."},{"key":"2020120306470583700_ref31","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1111\/j.1559-4564.2007.06159.x","article-title":"Islet amyloid and fibrosis in the cardiometabolic syndrome and type 2 diabetes mellitus","volume":"2","author":"Hayden","year":"2007","journal-title":"J. Cardiometab. Syndr."},{"key":"2020120306470583700_ref32","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/s41574-019-0206-6","article-title":"Insulin and the insulin receptor regulate gene expression","volume":"15","author":"Greenhill","year":"2019","journal-title":"Nat. Rev. Endocrinol."},{"key":"2020120306470583700_ref33","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.ajem.2017.07.070","article-title":"Anti-inflammatory and organ protective effect of insulin in scalded MODS rats without controlling hyperglycemia","volume":"36","author":"Zhu","year":"2018","journal-title":"Am. J. Emerg. Med."}],"container-title":["Microscopy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jmicro\/advance-article-pdf\/doi\/10.1093\/jmicro\/dfaa036\/33588647\/dfaa036.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jmicro\/article-pdf\/69\/6\/381\/34674574\/dfaa036.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jmicro\/article-pdf\/69\/6\/381\/34674574\/dfaa036.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T15:17:31Z","timestamp":1723216651000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jmicro\/article\/69\/6\/381\/5869662"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,10]]},"references-count":33,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,7,10]]},"published-print":{"date-parts":[[2020,12,3]]}},"URL":"https:\/\/doi.org\/10.1093\/jmicro\/dfaa036","relation":{},"ISSN":["2050-5698","2050-5701"],"issn-type":[{"value":"2050-5698","type":"print"},{"value":"2050-5701","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,12]]},"published":{"date-parts":[[2020,7,10]]}}}