{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:35:35Z","timestamp":1740170135907,"version":"3.37.3"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T00:00:00Z","timestamp":1596412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Since the majority of a tendon\u2019s dry weight is collagen fibers, tendon healing consists mainly of collagen repair and observing three-dimensional networks of collagen fibers with scanning electron microscopy (SEM) is optimal for investigating this process. In this report, a cell-maceration\/SEM method was used to investigate extrasynovial tendon (unwrapped tendon in synovial tissue such as the tendon sheath) healing of an injured Achilles tendon in a rat model. In addition, since mechanical stimulation is important for tendon healing, a novel, tensionless, rat lower leg tendon injury model was established and verified by visualizing the structural change of collagen fibers under tensionless conditions by SEM. This new model was created by transplanting the leg of a rat with a tendon laceration to the back, removing mechanical stimulation. We then compared the process of tendon healing with and without tension using SEM. Under tension, collagen at the tendon stump shows axial alignment and repair that subsequently demarcates the paratenon (connective tissue on the surface of an extrasynovial tendon) border. In contrast, under tensionless conditions, the collagen remains randomly arranged. Our findings demonstrate that mechanical stimulation contributes to axial arrangement and reinforces the importance of tendon tension in wound healing.<\/jats:p>","DOI":"10.1093\/jmicro\/dfaa043","type":"journal-article","created":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T11:09:42Z","timestamp":1595675382000},"page":"186-191","source":"Crossref","is-referenced-by-count":0,"title":["Visualization of microstructural change affected by mechanical stimulation in tendon healing with a novel tensionless model"],"prefix":"10.1093","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4814-0849","authenticated-orcid":false,"given":"Junya","family":"Oshima","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, University of Tsukuba, 1-1-1Tennodai, Tsukuba, Ibaraki 305-8577, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaoru","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, University of Tsukuba, 1-1-1Tennodai, Tsukuba, Ibaraki 305-8577, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoto","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Jichi Ika University Saitama Medical Center, 1-847, Amanumacho, Omiyaku, Saitama 330-8503, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoharu","family":"Kiyosawa","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuru","family":"Sekido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,8,3]]},"reference":[{"key":"2021032412094648700_R1","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1111\/j.2042-3306.1975.tb03221.x","article-title":"Structural and mechanical properties of tendon related to function","volume":"7","author":"Evans","year":"1975","journal-title":"Equine Vet. 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