{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:12:20Z","timestamp":1787497940257,"version":"build-2736575974"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T00:00:00Z","timestamp":1562198400000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15H05010"],"award-info":[{"award-number":["15H05010"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K20356"],"award-info":[{"award-number":["15K20356"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Promoting International Joint Research (Bilateral Collaborations) of JSPS"},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003769","name":"Eisai","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In this study, we aimed to clarify the role of ascorbic acid in collagen synthesis in periodontal ligaments using osteogenic disorder Shionogi (ODS)\/ShiJcl-od\/od rats lacking L-gulonolactone oxidase. These rats cannot synthesize ascorbic acid in vivo. Eight-week-old ODS\/ShiJcl-od\/od male rats were administered ascorbic acid solution at a concentration of 200\u00a0mg\/dL (control group, n\u00a0=\u00a06) or ascorbic acid solution at concentration of 0.3\u00a0mg\/dL (insufficient group, n\u00a0=\u00a012). Six rats of the insufficient group were then given with ascorbic acid solution at concentration of 200\u00a0mg\/dL for additional 3\u00a0weeks (rescued group, n\u00a0=\u00a06), and then, their mandibles were histochemically examined. Consequently, the insufficient group specimens were seen to possess fewer collagen fibers, and silver impregnation revealed numerous fine, reticular fiber-like fibrils branching off from collagen in the periodontal ligaments. In control group, faint immunoreactivities for matrix metalloproteinase (MMP)2 and cathepsin H were seen in the periphery of blood vessels and throughout the ligament, respectively. In contrast, in the insufficient group, intense MMP2-immunoreactivity was observed to be associated with collagen fibrils in the periodontal ligaments, and cathepsin H-immunopositivity was seen in ligamentous cells. The rescued group showed abundant collagen fibers filling the periodontal ligament space. Under transmission electron microscopy, ligamentous fibroblasts incorporated collagen fibrils into tubular endosomes\/lysosomes while simultaneously synthesizing collagen fibril bundles. Thus, ascorbic acid insufficiency affected the immunolocalization of cathepsin H and MMP2; however, ligamentous fibroblasts appear to possess the potential to synthesize collagen fibers when supplied with ascorbic acid.<\/jats:p>","DOI":"10.1093\/jmicro\/dfz021","type":"journal-article","created":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T07:08:13Z","timestamp":1556694493000},"page":"349-358","source":"Crossref","is-referenced-by-count":15,"title":["Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od\/od rats"],"prefix":"10.1093","volume":"68","author":[{"given":"Tomoka","family":"Hasegawa","sequence":"first","affiliation":[{"name":"Developmental Biology of Hard Tissue, Graduate School of Dental Medicine and Faculty of Dental Medicine, Hokkaido University, Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukina","family":"Miyamoto-Takasaki","sequence":"additional","affiliation":[{"name":"Developmental Biology of Hard Tissue, Graduate School of Dental Medicine and Faculty of Dental Medicine, Hokkaido University, Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Abe","sequence":"additional","affiliation":[{"name":"Developmental Biology of Hard Tissue, Graduate School of Dental Medicine and Faculty of Dental Medicine, Hokkaido University, Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Qiu","sequence":"additional","affiliation":[{"name":"Developmental Biology of Hard Tissue, Graduate School of Dental Medicine and Faculty of Dental Medicine, Hokkaido University, Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomaya","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Section of Dentistry, Japan Ground Self-Defense Forces Camp Asaka, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Yimin","sequence":"additional","affiliation":[{"name":"Department of Advanced Medicine, Graduate School of Medicine, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiji","family":"Yoshida","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, Hokkaido University, Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirona","family":"Yoshino","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, Hokkaido University, Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Hongo","sequence":"additional","affiliation":[{"name":"Developmental Biology of Hard Tissue, Graduate School of Dental Medicine and Faculty of Dental Medicine, Hokkaido University, Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayako","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Developmental Biology of Hard Tissue, Graduate School of Dental Medicine and Faculty of Dental Medicine, Hokkaido University, Sapporo Japan"},{"name":"Gerodontology, Graduate School of Dental Medicine and Faculty of Dental Medicine, Hokkaido University, Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneteru","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Applied Prosthodontics, Medical and Dental Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichiro","family":"Kuroshima","sequence":"additional","affiliation":[{"name":"Department of Applied Prosthodontics, Medical and Dental Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuniko","family":"Hara","sequence":"additional","affiliation":[{"name":"Pharmacological Evaluation Section, Eisai, Co. 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