{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:56:09Z","timestamp":1753876569138,"version":"3.41.2"},"reference-count":46,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,10,15]],"date-time":"2019-10-15T00:00:00Z","timestamp":1571097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>According to our previous reports, the intraperiodontal elastic fiber system comprises oxytalan fibers, whereas all types of elastic system fibers are present in the gingiva. Much remains to be elucidated regarding the topographic development of the elastic fiber system that constitutes the walls of the digestive organs. This study aimed to examine the topographic development of the elastic fiber system in the periodontal tissue, oral cavity and digestive tract of rats at light- and electron microscopic levels. At embryonic day 20, in situ hybridization revealed the mRNA expression of tropoelastin in the putative gingival lamina propria but not in the dental follicle. At the postnatal stage, the masticatory mucous membrane of the gingiva and hard palate comprised three different types of elastic system fibers (oxytalan, elaunin and elastic fibers). Conversely, the elastic fiber system comprised elaunin and elastic fibers in other oral mucosae and the lining mucosae of digestive tract organs (the esophagus, stomach and small intestine).<\/jats:p><jats:p>The findings of our study suggest that the elastic fiber system is mainly related to tissue resistance in the periodontal ligament and tissue elasticity in the oral mucosae without masticatory mucosae and the overlying mucosa of digestive tracts and both functions in the gingiva and hard palate, respectively. The appearance of elaunin fibers in the periodontium of rats aged 14\u00a0weeks suggests the expression of tropoelastin induced by mechanical stressors such as mastication. The intraperiodontal difference in the distribution of elaunin fibers suggests heterogeneity among fibroblasts constituting the periodontium.<\/jats:p>","DOI":"10.1093\/jmicro\/dfz030","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T15:44:04Z","timestamp":1562255044000},"source":"Crossref","is-referenced-by-count":0,"title":["Elastic fiber system evaluated in the digestive organ of rats"],"prefix":"10.1093","author":[{"given":"Kouji","family":"Inoue","sequence":"first","affiliation":[{"name":"Research Center of Electron Microscopy, Tsurumi University, Yokohama 230-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyuki","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Tissue and Cell Biology, School of Dental Medicine, Tsurumi University, Yokohama 230-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuji","family":"Sato","sequence":"additional","affiliation":[{"name":"Research Center of Electron Microscopy, Tsurumi University, Yokohama 230-8501, Japan"},{"name":"Department of Anatomy, Tissue and Cell Biology, School of Dental Medicine, Tsurumi University, Yokohama 230-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,10,15]]},"reference":[{"key":"2019101505284955200_ref1","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.aanat.2012.03.010","article-title":"Development of the oxytalan fiber system in the rat molar periodontal ligament evaluated by light- and electron-microscopic analyses","volume":"194","author":"Inoue","year":"2012","journal-title":"Ann. Anat."},{"key":"2019101505284955200_ref2","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.aanat.2013.03.012","article-title":"Development of the oxytalan fiber system in the periodontal space of rat incisors","volume":"195","author":"Inoue","year":"2013","journal-title":"Ann. Anat."},{"key":"2019101505284955200_ref3","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/j.archoralbio.2012.06.003","article-title":"The oxytalan fibre network in the periodontium and its possible mechanical function","volume":"57","author":"Strydom","year":"2012","journal-title":"Arch. Oral Biol."},{"key":"2019101505284955200_ref4","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/S0065-3233(05)70012-7","article-title":"Fibrillin microfibrils","volume":"70","author":"Kielty","year":"2005","journal-title":"Adv. Protein Chem."},{"key":"2019101505284955200_ref5","first-page":"297","article-title":"Comparative study between the elastic system fibers in human thin and thick skin","volume":"31","author":"Cotta-Pereira","year":"1978","journal-title":"Biol. Cell."},{"key":"2019101505284955200_ref6","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/BF00496583","article-title":"Distribution and organization of the elastic system fibres in healthy human gingiva","volume":"89","author":"Chavrier","year":"1988","journal-title":"Histochemistry."},{"key":"2019101505284955200_ref7","doi-asserted-by":"crossref","first-page":"2817","DOI":"10.1242\/jcs.115.14.2817","article-title":"Elastic fibres","volume":"115","author":"Kielty","year":"2002","journal-title":"J. Cell Sci."},{"key":"2019101505284955200_ref8","first-page":"137A","article-title":"A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy","volume":"27","author":"Karnovsky","year":"1965","journal-title":"J. Cell Biol."},{"key":"2019101505284955200_ref9","first-page":"287","article-title":"An improved electron stain for elastic fibers using tannic acid","volume":"24","author":"Kajikawa","year":"1975","journal-title":"J. Electron Microsc."},{"key":"2019101505284955200_ref10","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1083\/jcb.30.1.59","article-title":"The fine structure of elastic fibers","volume":"30","author":"Greenlee","year":"1966","journal-title":"J. Cell Biol."},{"key":"2019101505284955200_ref11","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1034\/j.1600-0765.2002.00662.x","article-title":"Expression of fibrillins and tropoelastin by human gingival and periodontal ligament fibroblasts in vitro","volume":"37","author":"Tsuruga","year":"2002","journal-title":"J. Periodontal Res."},{"key":"2019101505284955200_ref12","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1177\/0810198","article-title":"Tropoelastin expression by periodontal fibroblasts","volume":"81","author":"Tsuruga","year":"2002","journal-title":"J. Dental Res."},{"key":"2019101505284955200_ref13","first-page":"172","article-title":"Elastic fibre assembly: macromolecular interactions","volume":"192","author":"Mecham","year":"1995","journal-title":"Ciba Found. Symp."},{"key":"2019101505284955200_ref14","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/1523-1747.ep12481882","article-title":"Oxytalan, elaunin, and elastic fibers in the human skin","volume":"66","author":"Cotta-Pereira","year":"1976","journal-title":"J. Invest. Dermatol."},{"key":"2019101505284955200_ref15","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1369\/jhc.5A6905.2006","article-title":"Immunohistochemical characterization of elastic system fibers in rat molar periodontal ligament","volume":"54","author":"Sawada","year":"2006","journal-title":"J. Histochem. Cytochem."},{"key":"2019101505284955200_ref16","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1083\/jcb.28.1.33","article-title":"The fine structure of the oxytalan fiber","volume":"28","author":"Carmichael","year":"1966","journal-title":"J. Cell Biol."},{"key":"2019101505284955200_ref17","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/j.1600-0714.1974.tb01724.x","article-title":"Oxytalan connective tissue fibers: a review","volume":"3","author":"Fullmer","year":"1974","journal-title":"J. Oral Pathol."},{"key":"2019101505284955200_ref18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S146239940600007X","article-title":"Elastic fibres in health and disease","volume":"8","author":"Kielty","year":"2006","journal-title":"Expert. Rev. Mol. Med."},{"key":"2019101505284955200_ref19","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1152\/physiolgenomics.00114.2002","article-title":"Loci of intestinal distress in cystic fibrosis knockout mice","volume":"12","author":"Haston","year":"2003","journal-title":"Physiol. Genomics."},{"key":"2019101505284955200_ref20","doi-asserted-by":"crossref","first-page":"3819","DOI":"10.1242\/jeb.01765","article-title":"The modulus of elasticity of fibrillin-containing elastic fibres in the mesoglea of the hydromedusa Polyorchis penicillatus","volume":"208","author":"Megill","year":"2005","journal-title":"J. Exp. Biol."},{"key":"2019101505284955200_ref21","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/0002-9416(68)90199-1","article-title":"A study of the periodontium during orthodontic rotation of teeth","volume":"54","author":"Edwards","year":"1968","journal-title":"Am. J. Orthod."},{"key":"2019101505284955200_ref22","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/0002-9416(69)90278-4","article-title":"Increased stability of orthodontically rotated teeth following gingivectomy in Macaca nemestrina","volume":"56","author":"Boese","year":"1969","journal-title":"Am. J. Orthod."},{"key":"2019101505284955200_ref23","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/0002-9416(76)90259-1","article-title":"Reconstitution of the human oxytalan system during orthodontic tooth movement","volume":"70","author":"Sims","year":"1976","journal-title":"Am. J. Orthod."},{"key":"2019101505284955200_ref24","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1093\/ejo\/10.1.13","article-title":"A quantitative study of oxytalan fibres in the transseptal region and tension zones of rat molars following orthodontic movement","volume":"10","author":"Bowling","year":"1988","journal-title":"Eur. J. Orthod."},{"key":"2019101505284955200_ref25","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1177\/28.3.7354216","article-title":"Reaction of oxytalan fibers in human periodontium to mechanical stress. A combined histochemical and morphometric analysis","volume":"28","author":"Jonas","year":"1980","journal-title":"J. Histochem. Cytochem."},{"key":"2019101505284955200_ref26","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0167-4889(95)00090-F","article-title":"Rab and rho GTPases are involved in specific response of periodontal ligament fibroblasts to mechanical stretching","volume":"1268","author":"Basdra","year":"1995","journal-title":"Biochim. Biophys. Acta."},{"key":"2019101505284955200_ref27","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/S0889-5406(96)70147-6","article-title":"Intracellular calcium response to hydraulic pressure in human periodontal ligament fibroblasts","volume":"109","author":"Nakago-Matsuo","year":"1996","journal-title":"Am. J. Orthod. Dentofacial. Orthop."},{"key":"2019101505284955200_ref28","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1111\/j.1600-0765.2008.01099.x","article-title":"Stretching modulates oxytalan fibers in human periodontal ligament cells","volume":"44","author":"Tsuruga","year":"2009","journal-title":"J. Periodontal Res."},{"key":"2019101505284955200_ref29","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0003-9969(84)90100-6","article-title":"Histological study, including ultrastructural quantification, of the periodontal ligament in the lathyritic rat mandibular dentition","volume":"29","author":"Shore","year":"1984","journal-title":"Arch. Oral Biol."},{"key":"2019101505284955200_ref30","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1600-0765.2008.01196.x","article-title":"Immunohistochemical localization of elastin, fibrillins and microfibril-associated glycoprotein-1 in the developing periodontal ligament of the rat molar","volume":"45","author":"Sugawara","year":"2010","journal-title":"J. Periodontal Res."},{"key":"2019101505284955200_ref31","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.acthis.2009.09.002","article-title":"Elastic system fibers in rat incisor periodontal ligament\u2014immunohistochemical study using sections of fresh-frozen un-demineralized tissues","volume":"113","author":"Kondo","year":"2011","journal-title":"Acta. Histochem."},{"key":"2019101505284955200_ref32","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1177\/0022034509338914","article-title":"On a path to unfolding the biological mechanisms of orthodontic tooth movement","volume":"88","author":"Krishnan","year":"2009","journal-title":"J. Dent. Res."},{"key":"2019101505284955200_ref33","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.ajodo.2007.10.030","article-title":"Effect of duration of force application on blood vessels in young and adult rats","volume":"133","author":"Ren","year":"2008","journal-title":"Am. J. Orthod. Dentofacial. Orthop."},{"key":"2019101505284955200_ref34","first-page":"545","article-title":"An immunohistochemical, histological, and electron-microscopic study of the human periodontal ligament during orthodontic treatment","volume":"21","author":"Anastasi","year":"2008","journal-title":"Int. J. Mol. Med."},{"key":"2019101505284955200_ref35","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1111\/j.1600-0765.1998.tb02350.x","article-title":"Mechanical forces alter extracellular matrix synthesis by human periodontal ligament fibroblasts","volume":"33","author":"Howard","year":"1998","journal-title":"J. Periodontal Res."},{"key":"2019101505284955200_ref36","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1177\/10454411910020040101","article-title":"Tooth movement","volume":"2","author":"Davidovitch","year":"1991","journal-title":"Crit. Rev. Oral Biol. Med."},{"key":"2019101505284955200_ref37","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1093\/ejo\/19.1.29","article-title":"Collagen synthesis from human PDL cells following orthodontic tooth movement","volume":"19","author":"Bumann","year":"1997","journal-title":"Eur. J. Orthod."},{"key":"2019101505284955200_ref38","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s00011-003-1243-z","article-title":"Neuropeptides stimulate production of interleukin-1\u03b2, interleukin-6, and tumor necrosis factor-\u03b1 in human dental pulp cells","volume":"53","author":"Yamaguchi","year":"2004","journal-title":"Inflamm. Res."},{"key":"2019101505284955200_ref39","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.archoralbio.2003.08.002","article-title":"Expression of tropoelastin in human periodontal ligament fibroblasts after simulation of orthodontic force","volume":"49","author":"Redlich","year":"2004","journal-title":"Arch. Oral Biol."},{"key":"2019101505284955200_ref40","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1177\/00220345880670011301","article-title":"A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro","volume":"67","author":"Somerman","year":"1988","journal-title":"J. Dent. Res."},{"key":"2019101505284955200_ref41","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1177\/00220345890680050201","article-title":"Initial characterization of cells derived from human periodontia","volume":"68","author":"Piche","year":"1989","journal-title":"J. Dent. Res."},{"key":"2019101505284955200_ref42","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1902\/jop.1991.62.8.499","article-title":"Human periodontal cells initiate mineral-like nodules in vitro","volume":"62","author":"Arceo","year":"1991","journal-title":"J. Periodontol."},{"key":"2019101505284955200_ref43","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1111\/j.1600-0765.2008.01148.x","article-title":"The defensive role of lysozyme in human gingiva in inflammatory periodontal disease","volume":"44","author":"Younes","year":"2009","journal-title":"J. Periodontal Res."},{"key":"2019101505284955200_ref44","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s007840050026","article-title":"Morphometric analysis of collagen and elastic fibers in normal skin and gingiva in relation to age","volume":"1","author":"Gogly","year":"1997","journal-title":"Clin. Oral Invest."},{"key":"2019101505284955200_ref45","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-1-4684-9093-0_10","article-title":"Recent observations on the structure and composition of elastin","volume":"79","author":"Gotte","year":"1977","journal-title":"Adv. Exp. Med. Biol."},{"key":"2019101505284955200_ref46","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1177\/21.3.199","article-title":"The elastic fiber. A review","volume":"21","author":"Ross","year":"1973","journal-title":"J. Histochem. Cytochem."}],"container-title":["Microscopy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jmicro\/advance-article-pdf\/doi\/10.1093\/jmicro\/dfz030\/30145940\/dfz030.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jmicro\/advance-article-pdf\/doi\/10.1093\/jmicro\/dfz030\/30145940\/dfz030.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T23:14:53Z","timestamp":1663888493000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jmicro\/advance-article\/doi\/10.1093\/jmicro\/dfz030\/5586241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,15]]},"references-count":46,"URL":"https:\/\/doi.org\/10.1093\/jmicro\/dfz030","relation":{},"ISSN":["2050-5698","2050-5701"],"issn-type":[{"type":"print","value":"2050-5698"},{"type":"electronic","value":"2050-5701"}],"subject":[],"published":{"date-parts":[[2019,10,15]]},"article-number":"dfz030"}}