{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:40:02Z","timestamp":1772138402917,"version":"3.50.1"},"reference-count":35,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T00:00:00Z","timestamp":1573603200000},"content-version":"vor","delay-in-days":2,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Japan Society for the Promotion of Science JSPS","award":["19K16666"],"award-info":[{"award-number":["19K16666"]}]},{"name":"Japan Society for the Promotion of Science JSPS","award":["18J01631"],"award-info":[{"award-number":["18J01631"]}]},{"name":"AMED Research Program on Emerging and Re-emerging Infectious Diseases"},{"name":"AMED Japanese Initiative for Progress of Research on Infectious Disease for Global Epidemic"},{"name":"JSPS Core-to-Core Program A, the Advanced Research Networks"},{"name":"Grant for Joint Research Project of the Institute of Medical Science, University of Tokyo"},{"name":"Joint Usage\/Research Center Program of the Institute for Frontier Life and Medical Sciences Kyoto University"},{"DOI":"10.13039\/501100005927","name":"Daiichi Sankyo Foundation of Life Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005927","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Joint Usage\/Research Center on Tropical Disease"},{"name":"Institute of Tropical Medicine and Nagasaki University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lassa virus (LASV), belonging to the family Arenaviridae, causes severe haemorrhagic manifestations and is associated with a high mortality rate in humans. Thus, it is classified as a biosafety level (BSL)-4 agent. Since countermeasures for LASV diseases are yet to be developed, it is important to elucidate the molecular mechanisms underlying the life cycle of the virus, including its viral and host cellular protein interactions. These underlying molecular mechanisms may serve as the key for developing novel therapeutic options. Lymphocytic choriomeningitis virus (LCMV), a close relative of LASV, is usually asymptomatic and is categorized as a BSL-2 agent. In the present study, we visualized the transport of viral matrix Z protein in LCMV-infected cells using live-cell imaging microscopy. We demonstrated that the transport of Z protein is mediated by polymerized microtubules. Interestingly, the transport of LASV Z protein showed characteristics similar to those of Z protein in LCMV-infected cells. The live-cell imaging system using LCMV provides an attractive surrogate measure for studying arenavirus matrix protein transport in BSL-2 laboratories. In addition, it could be also utilized to analyze the interactions between viral matrix proteins and the cellular cytoskeleton, as well as to evaluate the antiviral compounds that target the transport of viral matrix proteins.<\/jats:p>","DOI":"10.1093\/jmicro\/dfz034","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T07:27:47Z","timestamp":1569482867000},"source":"Crossref","is-referenced-by-count":3,"title":["Microtubule-dependent transport of arenavirus matrix protein demonstrated using live-cell imaging microscopy"],"prefix":"10.1093","author":[{"given":"Yuki","family":"Takamatsu","sequence":"first","affiliation":[{"name":"Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University, Japan"},{"name":"Institut f\u00fcr Virologie, Philipps-Universit\u00e4t Marburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junichi","family":"Kajikawa","sequence":"additional","affiliation":[{"name":"Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Muramoto","sequence":"additional","affiliation":[{"name":"Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Nakano","sequence":"additional","affiliation":[{"name":"Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0658-4663","authenticated-orcid":false,"given":"Takeshi","family":"Noda","sequence":"additional","affiliation":[{"name":"Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,11]]},"reference":[{"key":"2019111315302672100_ref1","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1007\/s00705-018-04127-3","article-title":"Taxonomy of the order Bunyavirales: second update 2018","volume":"164","author":"Maes","year":"2019","journal-title":"Arch. 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Microbiol."},{"key":"2019111315302672100_ref28","doi-asserted-by":"crossref","first-page":"S160","DOI":"10.1093\/infdis\/jiv083","article-title":"Transport of ebolavirus nucleocapsids is dependent on actin polymerization: live-cell imaging analysis of Ebolavirus-infected cells","volume":"212","author":"Schudt","year":"2015","journal-title":"J. Infect. Dis."},{"key":"2019111315302672100_ref29","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/S0966-842X(00)01824-2","article-title":"Mechanisms of viral transport in the cytoplasm","volume":"8","author":"Sodeik","year":"2000","journal-title":"Trends Microbiol."},{"key":"2019111315302672100_ref30","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1146\/annurev.cellbio.18.012502.105920","article-title":"Break ins and break outs: viral interactions with the cytoskeleton of mammalian cells","volume":"18","author":"Smith","year":"2002","journal-title":"Annu. Rev. Cell Dev. 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