{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:47:35Z","timestamp":1760550455834,"version":"3.44.0"},"reference-count":39,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[1986,12,1]],"date-time":"1986-12-01T00:00:00Z","timestamp":533779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[1986,12,1]],"date-time":"1986-12-01T00:00:00Z","timestamp":533779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Nutrition"],"published-print":{"date-parts":[[1986,12]]},"DOI":"10.1093\/jn\/116.12.2424","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T10:51:28Z","timestamp":1515581488000},"page":"2424-2431","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"title":["Effects of Whole Rat Embryos Cultured on Serum from Zinc- and Copper-Deficient Rats"],"prefix":"10.1016","volume":"116","author":[{"given":"Gregory D.","family":"Mieden","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl L.","family":"Keen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucille S.","family":"Hurley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman W.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1093\/jn\/116.12.2424_bib1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1289\/ehp.7618105","article-title":"Techniques for assessment of teratologic effects: embryo culture","volume":"18","author":"New","year":"1976","journal-title":"Environ. Health Perspect"},{"key":"10.1093\/jn\/116.12.2424_bib2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1530\/jrf.0.0480219","article-title":"Improved development of head-fold rat embryos in culture resulting from low oxygen and modification of the culture serum","volume":"48","author":"New","year":"1976","journal-title":"J. Reprod. Fertil"},{"key":"10.1093\/jn\/116.12.2424_bib3","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1002\/tera.1420170306","article-title":"Abnormalities induced in cultured rat embryos by hyperthermia","volume":"17","author":"Cockroft","year":"1978","journal-title":"Teratology"},{"key":"10.1093\/jn\/116.12.2424_bib4","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.2337\/diab.32.11.1070","article-title":"Effects of maternal diabetes on early embryogenesis. The role of insulin and insulin therapy","volume":"32","author":"Sadler","year":"1983","journal-title":"Diabetes"},{"key":"10.1093\/jn\/116.12.2424_bib5","first-page":"543","article-title":"Effect of yolk-sac antibody on rat embryos grown in culture","volume":"27","author":"New","year":"1972","journal-title":"J. Embryol. Exp. Morphol"},{"key":"10.1093\/jn\/116.12.2424_bib6","first-page":"707","article-title":"Serum variants causing the formation of double hearts and other abnormalities in explanted rat embryos in culture","volume":"31","author":"Steele","year":"1974","journal-title":"J. Embryol. Exp. Morphol"},{"key":"10.1093\/jn\/116.12.2424_bib7","first-page":"347","article-title":"Analysis of sera from monkeys with histories of fetal wastage and the identification of teratogenicity in sera in human chronic spontaneous aborters using rat embryo cultures","volume":"1","author":"Carey","year":"1983","journal-title":"Trophoblast Res"},{"key":"10.1093\/jn\/116.12.2424_bib8","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1126\/science.7053560","article-title":"Serum from monkey with histories of fetal wastage causes abnormalities in cultured rat embryos","volume":"215","author":"Klein","year":"1982","journal-title":"Science"},{"key":"10.1093\/jn\/116.12.2424_bib9","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/tera.1420210211","article-title":"The use of cultured rat embryos to evaluate the teratogenic activity of serum: cadmium and cyclophosphamide","volume":"21","author":"Klein","year":"1980","journal-title":"Teratology"},{"key":"10.1093\/jn\/116.12.2424_bib10","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1016\/0024-3205(82)90719-6","article-title":"Interactions of cadmium and zinc in cultured rat embryos","volume":"31","author":"Record","year":"1982","journal-title":"Life Sci"},{"key":"10.1093\/jn\/116.12.2424_bib11","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1002\/tera.1420300107","article-title":"Zinc amelioration of cadmium induced teratogenesis in vitro","volume":"30","author":"Warner","year":"1984","journal-title":"Teratology"},{"key":"10.1093\/jn\/116.12.2424_bib12","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0300-483X(83)90001-X","article-title":"A comparison of the in vivo and in vitro responses of mammalian embryos to a teratogenic insult","volume":"28","author":"Warner","year":"1983","journal-title":"Toxicology"},{"key":"10.1093\/jn\/116.12.2424_bib13","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1126\/science.7361097","article-title":"Successful culture of rat embryos on human serum: use in the detection of teratogens","volume":"207","author":"Chatot","year":"1980","journal-title":"Science"},{"key":"10.1093\/jn\/116.12.2424_bib14","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/j.1528-1157.1984.tb04177.x","article-title":"Human serum teratogenicity studied by rat embryo culture: epilepsy, anticonvulsant drugs, and nutrition","volume":"25","author":"Chatot","year":"1984","journal-title":"Epilepsia"},{"key":"10.1093\/jn\/116.12.2424_bib15","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1002\/tera.1420110307","article-title":"The use of in vitro procedures in teratology","volume":"11","author":"Kochhar","year":"1975","journal-title":"Teratology"},{"key":"10.1093\/jn\/116.12.2424_bib16","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1056\/NEJM198401263100404","article-title":"The honeybee syndrome implications of the teratogenicity of mannose in rat-embryo cultures","volume":"310","author":"Freinkel","year":"1984","journal-title":"N. Engl. J. Med"},{"key":"10.1093\/jn\/116.12.2424_bib17","first-page":"505","article-title":"The effect of excess vitamin A on the development of rat embryos in culture","volume":"32","author":"Morriss","year":"1974","journal-title":"J. Embryol. Exp. Morphol"},{"key":"10.1093\/jn\/116.12.2424_bib18","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1530\/jrf.0.0380115","article-title":"The effect of vitamin E and synthetic antioxidants on the growth in vitro of explanted rat embryos","volume":"38","author":"Steele","year":"1974","journal-title":"J. Reprod. Fertil"},{"key":"10.1093\/jn\/116.12.2424_bib19","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1152\/physrev.1981.61.2.249","article-title":"Teratogenic aspects of manganese, zinc and copper nutrition","volume":"61","author":"Hurley","year":"1981","journal-title":"Physiol. Rev"},{"key":"10.1093\/jn\/116.12.2424_bib20","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/S0368-1742(60)80010-0","article-title":"Reproductive failure in rats due to copper deficiency","volume":"70","author":"Dutt","year":"1960","journal-title":"J. Comp. Pathol"},{"key":"10.1093\/jn\/116.12.2424_bib21","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1079\/BJN19690007","article-title":"The effect of copper on reproduction in the female rat","volume":"23","author":"Hall","year":"1969","journal-title":"Br. J. Nutr"},{"key":"10.1093\/jn\/116.12.2424_bib22","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1093\/jn\/97.1.42","article-title":"Neural lesions in the offspring of female rats fed a copper-deficient diet","volume":"7","author":"Carlton","year":"1969","journal-title":"J. Nutr"},{"key":"10.1093\/jn\/116.12.2424_bib23","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1093\/jn\/73.2.151","article-title":"Mineral deficiencies of milk and \u221egenital malformations in rats","volume":"73","author":"O\u2019Dell","year":"1961","journal-title":"J. Nutr"},{"key":"10.1093\/jn\/116.12.2424_bib24","doi-asserted-by":"crossref","unstructured":"Keen, C. L., L\u00f6nnerdal,B. & Hurley, L. S. (1982) Teratogenic effects of copper deficiency and excess. In: Inflammatory Diseases of Copper (Sorenson, J. R. J., ed.), pp. 109-121, Humana Press, Clifton, NJ.","DOI":"10.1007\/978-1-4612-5829-2_11"},{"key":"10.1093\/jn\/116.12.2424_bib25","first-page":"17","article-title":"Teratogenic effects of D-penicillamine in rats: relation to copper deficiency","volume":"2","author":"Keen","year":"1983","journal-title":"Drug-Nutr. Interact"},{"key":"10.1093\/jn\/116.12.2424_bib26","doi-asserted-by":"crossref","first-page":"598","DOI":"10.3181\/00379727-173-41693","article-title":"Teratogenesis and low copper status resulting from triethylenetetramine in rats","volume":"173","author":"Keen","year":"1983","journal-title":"Proc. Soc. Exp. Biol. Med"},{"key":"10.1093\/jn\/116.12.2424_bib27","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1093\/jn\/113.3.501","article-title":"Reduction by copper supplementation of teratogenic effects of D-penicillamine","volume":"113","author":"Mark-Savage","year":"1983","journal-title":"J. Nutr"},{"key":"10.1093\/jn\/116.12.2424_bib28","first-page":"203","article-title":"The effect of copper supplementation on the teratogenic effects of triethylenetetramine","volume":"2","author":"Cohen","year":"1983","journal-title":"Drug-Nutr. Interact"},{"key":"10.1093\/jn\/116.12.2424_bib29","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1093\/jn\/113.7.1448","article-title":"Comparative aspects of dietary copper and zinc deficiencies in pregnant rats","volume":"113","author":"Masters","year":"1983","journal-title":"J. Nutr"},{"key":"10.1093\/jn\/116.12.2424_bib30","first-page":"686","article-title":"Age and sex alter the effects of zinc deficiency of food intake in rats","volume":"43","author":"Kawamoto","year":"1984","journal-title":"Fed. Proc"},{"key":"10.1093\/jn\/116.12.2424_bib31","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1093\/jn\/115.6.710","article-title":"Determinants of copper deficiency anemia in rats","volume":"115","author":"Cohen","year":"1985","journal-title":"J. Nutr"},{"key":"10.1093\/jn\/116.12.2424_bib32","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1530\/jrf.0.0350135","article-title":"Culture of explanted rat embryos in rotating tubes","volume":"35","author":"New","year":"1973","journal-title":"J. Reprod. Fertil"},{"key":"10.1093\/jn\/116.12.2424_bib33","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin-phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem"},{"key":"10.1093\/jn\/116.12.2424_bib34","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/BF02990451","article-title":"Influence of ashing techniques on the analysis of trace elements. I. Wet ashing","volume":"3","author":"Clegg","year":"1981","journal-title":"Biol. Trace Element Res"},{"key":"10.1093\/jn\/116.12.2424_bib35","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1126\/science.7022654","article-title":"The essential trace elements","volume":"213","author":"Mertz","year":"1981","journal-title":"Science"},{"key":"10.1093\/jn\/116.12.2424_bib36","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1093\/jn\/95.1.8","article-title":"Severe zinc deficiency in male and female rats","volume":"95","author":"Swenerton","year":"1968","journal-title":"J. Nutr"},{"key":"10.1093\/jn\/116.12.2424_bib37","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1093\/jn\/102.3.343","article-title":"Effect of zinc deprivation from day 12,15 or 18 of gestation on parturition in the rat","volume":"102","author":"Apgar","year":"1972","journal-title":"J. Nutr"},{"key":"10.1093\/jn\/116.12.2424_bib38","first-page":"645","article-title":"Cogenital malformations of the brain produced by short term deficiencies in rats","volume":"77","author":"Warkany","year":"1973","journal-title":"Am. J. Ment. Defic"},{"key":"10.1093\/jn\/116.12.2424_bib39","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/tera.1420040211","article-title":"Teratognic effects of short-term and transitory zinc deficiency in rats","volume":"4","author":"Hurley","year":"1971","journal-title":"Teratology"}],"container-title":["The Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022316623080185?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022316623080185?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/academic.oup.com\/jn\/article-pdf\/116\/12\/2424\/23997099\/jn1160122424.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T05:49:42Z","timestamp":1758347382000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022316623080185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,12]]},"references-count":39,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1986,12]]}},"alternative-id":["S0022316623080185"],"URL":"https:\/\/doi.org\/10.1093\/jn\/116.12.2424","relation":{},"ISSN":["0022-3166"],"issn-type":[{"type":"print","value":"0022-3166"}],"subject":[],"published":{"date-parts":[[1986,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effects of Whole Rat Embryos Cultured on Serum from Zinc- and Copper-Deficient Rats","name":"articletitle","label":"Article Title"},{"value":"The Journal of Nutrition","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1093\/jn\/116.12.2424","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 1986 American Society for Nutrition. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}