{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T01:02:59Z","timestamp":1768438979648,"version":"3.49.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Institutes of Health\/National Institute on Aging with awards","award":["1RF1AG059789"],"award-info":[{"award-number":["1RF1AG059789"]}]},{"name":"National Institutes of Health\/National Institute on Aging with awards","award":["1RF1AG058674"],"award-info":[{"award-number":["1RF1AG058674"]}]},{"name":"National Institutes of Health\/National Institute on Aging with awards","award":["1P30AG062421"],"award-info":[{"award-number":["1P30AG062421"]}]},{"DOI":"10.13039\/100000957","name":"Alzheimer\u2019s Association","doi-asserted-by":"crossref","award":["2018-AARF-591935"],"award-info":[{"award-number":["2018-AARF-591935"]}],"id":[{"id":"10.13039\/100000957","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The protein tau, when misfolded in neurodegenerative diseases, has several prion-like properties including being able to spread by cell-to-cell transfer, induce templated seeding, and exist in distinct conformational strains. These properties of transmission may present health hazards when lesion-containing biospecimens are used in research and neuropathology laboratories. We evaluated the impact standard sterilization and cleaning methods have on the capacity of tau seeds to induce aggregation. We employed a previously developed, highly sensitive FRET-based biosensor assay to assess remnant tau seeding after exposure to these procedures. For tau species derived from human Alzheimer disease tissue (brain homogenate and sarkosyl-insoluble fibrils), both autoclaving and incubation in 90.6% formic acid were sufficient to reduce tau bioactivity. By contrast, boiling was not always effective in completely blocking bioactivity in the seeding assay. Notably, only formic acid incubation was able to produce a similar reduction in tissue from a P301L mutant tau mouse model of tauopathy. Our study highlights nuances in methods for inactivation of tau seeding which may support adapted tissue processing procedures, especially in research settings. These findings also highlight the importance of universal precautions when handling human neuropathological and research laboratory materials.<\/jats:p>","DOI":"10.1093\/jnen\/nlab087","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T07:57:57Z","timestamp":1631174277000},"page":"912-921","source":"Crossref","is-referenced-by-count":3,"title":["Impact of Sterilization Methods on the Seeding Ability of Human Tau Proteopathic Seeds"],"prefix":"10.1093","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3906-7806","authenticated-orcid":false,"given":"Analiese R","family":"Fernandes","sequence":"first","affiliation":[{"name":"From the Department of Neurology, Massachusetts General Hospital , Charlestown, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5614-341X","authenticated-orcid":false,"given":"Simon","family":"Dujardin","sequence":"additional","affiliation":[{"name":"From the Department of Neurology, Massachusetts General Hospital , Charlestown, Massachusetts, USA"},{"name":"Harvard Medical School , Boston, Massachusetts, USA (SD, AMdG, BTH, MPF)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasie","family":"Mat\u00e9 de G\u00e9rando","sequence":"additional","affiliation":[{"name":"From the Department of Neurology, Massachusetts General Hospital , Charlestown, Massachusetts, USA"},{"name":"Harvard Medical School , Boston, Massachusetts, USA (SD, AMdG, BTH, MPF)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley T","family":"Hyman","sequence":"additional","affiliation":[{"name":"From the Department of Neurology, Massachusetts General Hospital , Charlestown, Massachusetts, USA"},{"name":"Harvard Medical School , Boston, Massachusetts, USA (SD, AMdG, BTH, MPF)"},{"name":"C.S. Kubik Laboratory for Neuropathology, Department of Pathology , Massachusetts General Hospital, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew P","family":"Frosch","sequence":"additional","affiliation":[{"name":"From the Department of Neurology, Massachusetts General Hospital , Charlestown, Massachusetts, USA"},{"name":"Harvard Medical School , Boston, Massachusetts, USA (SD, AMdG, BTH, MPF)"},{"name":"C.S. Kubik Laboratory for Neuropathology, Department of Pathology , Massachusetts General Hospital, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,9,8]]},"reference":[{"key":"2024032523420366700_nlab087-B1","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1126\/science.6801762","article-title":"Novel proteinaceous infectious particles cause scrapie","volume":"216","author":"Prusiner","year":"1982","journal-title":"Science"},{"key":"2024032523420366700_nlab087-B2","doi-asserted-by":"crossref","first-page":"13363","DOI":"10.1073\/pnas.95.23.13363","article-title":"Prions","volume":"95","author":"Prusiner","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2024032523420366700_nlab087-B3","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1146\/annurev-genet-110711-155524","article-title":"Biology and genetics of prions causing neurodegeneration","volume":"47","author":"Prusiner","year":"2013","journal-title":"Annu Rev Genet"},{"key":"2024032523420366700_nlab087-B4","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1152\/physrev.00006.2009","article-title":"Prions: Protein aggregation and infectious diseases","volume":"89","author":"Aguzzi","year":"2009","journal-title":"Physiol Rev"},{"key":"2024032523420366700_nlab087-B5","doi-asserted-by":"crossref","first-page":"223","DOI":"10.4161\/pri.19912","article-title":"Is tau ready for admission to the prion club?","volume":"6","author":"Hall","year":"2012","journal-title":"Prion"},{"key":"2024032523420366700_nlab087-B6","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/nrn2786","article-title":"Prion-like mechanisms in neurodegenerative diseases","volume":"11","author":"Frost","year":"2010","journal-title":"Nat Rev Neurosci"},{"key":"2024032523420366700_nlab087-B7","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1016\/j.cell.2012.02.022","article-title":"The amyloid state of proteins in human diseases","volume":"148","author":"Eisenberg","year":"2012","journal-title":"Cell"},{"key":"2024032523420366700_nlab087-B8","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1146\/annurev-biochem-090908-120656","article-title":"Amyloid structure: Conformational diversity and consequences","volume":"80","author":"Toyama","year":"2011","journal-title":"Annu Rev Biochem"},{"key":"2024032523420366700_nlab087-B9","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1126\/science.1105850","article-title":"Self-propagating, molecular-level polymorphism in Alzheimer\u2019s \u00df-amyloid fibrils","volume":"307","author":"Petkova","year":"2005","journal-title":"Science"},{"key":"2024032523420366700_nlab087-B10","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/nature12481","article-title":"Self-propagation of pathogenic protein aggregates in neurodegenerative diseases","volume":"501","author":"Jucker","year":"2013","journal-title":"Nature"},{"key":"2024032523420366700_nlab087-B11","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1126\/science.1131864","article-title":"Exogenous induction of cerebral \u00df-amyloidogenesis is governed by agent and host","volume":"313","author":"Meyer-Luehmann","year":"2006","journal-title":"Science"},{"key":"2024032523420366700_nlab087-B12","doi-asserted-by":"crossref","first-page":"2225","DOI":"10.1016\/j.neurobiolaging.2011.06.022","article-title":"Prion-like acceleration of a synucleinopathy in a transgenic mouse model","volume":"33","author":"Mougenot","year":"2012","journal-title":"Neurobiol Aging"},{"key":"2024032523420366700_nlab087-B13","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1084\/jem.20112457","article-title":"Intracerebral inoculation of pathological \u03b1-synuclein initiates a rapidly progressive neurodegenerative \u03b1-synucleinopathy in mice","volume":"209","author":"Luk","year":"2012","journal-title":"J Exp Med"},{"key":"2024032523420366700_nlab087-B14","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/S1474-4422(20)30238-6","article-title":"Potential human transmission of amyloid \u03b2 pathology: Surveillance and risks","volume":"19","author":"Lauwers","year":"2020","journal-title":"Lancet Neurol"},{"key":"2024032523420366700_nlab087-B15","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/s41586-018-0104-4","article-title":"Cellular milieu imparts distinct pathological \u03b1-synuclein strains in \u03b1-synucleinopathies","volume":"557","author":"Peng","year":"2018","journal-title":"Nature"},{"key":"2024032523420366700_nlab087-B16","doi-asserted-by":"crossref","first-page":"fcaa010","DOI":"10.1093\/braincomms\/fcaa010","article-title":"Analysis of \u03b1-synuclein species enriched from cerebral cortex of humans with sporadic dementia with Lewy bodies","volume":"2","author":"Sanderson","year":"2020","journal-title":"Brain Commun"},{"key":"2024032523420366700_nlab087-B17","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/s41593-019-0541-x","article-title":"\u03b1-Synuclein strains target distinct brain regions and cell types","volume":"23","author":"Lau","year":"2020","journal-title":"Nat Neurosci"},{"key":"2024032523420366700_nlab087-B18","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.neuron.2011.11.033","article-title":"Propagation of tau pathology in a model of early Alzheimer\u2019s disease","volume":"73","author":"De Calignon","year":"2012","journal-title":"Neuron"},{"key":"2024032523420366700_nlab087-B19","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/978-981-32-9358-8_23","article-title":"Tau prion-like propagation: State of the art and current challenges","volume":"1184","author":"Dujardin","year":"2019","journal-title":"Adv Exp Med Biol"},{"key":"2024032523420366700_nlab087-B20","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/2051-5960-2-14","article-title":"Acta Neuropathologica Communications","volume":"2","author":"Dujardin","year":"2014","journal-title":"Acta Neuropathol Commun"},{"key":"2024032523420366700_nlab087-B21","doi-asserted-by":"crossref","first-page":"E4376","DOI":"10.1073\/pnas.1411649111","article-title":"Proteopathic tau seeding predicts tauopathy in vivo","volume":"111","author":"Holmes","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2024032523420366700_nlab087-B22","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1016\/j.neuron.2014.04.047","article-title":"Distinct tau prion strains propagate in cells and mice and define different tauopathies","volume":"82","author":"Sanders","year":"2014","journal-title":"Neuron"},{"key":"2024032523420366700_nlab087-B23","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1038\/s41591-020-0938-9","article-title":"Tau molecular diversity contributes to clinical heterogeneity in Alzheimer\u2019s disease","volume":"26","author":"Dujardin","year":"2020","journal-title":"Nat Med"},{"key":"2024032523420366700_nlab087-B24","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/s41586-019-1026-5","article-title":"Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules","volume":"568","author":"Falcon","year":"2019","journal-title":"Nature"},{"key":"2024032523420366700_nlab087-B25","first-page":"163","article-title":"Distinct conformers of assembled tau in Alzheimer\u2019s and Pick\u2019s diseases","volume":"2018","author":"Goedert","journal-title":"Cold Spring Harb Symp Quant Biol"},{"key":"2024032523420366700_nlab087-B26","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/j.neuron.2016.09.055","article-title":"Tau prion strains dictate patterns of cell pathology, progression rate, and regional vulnerability in vivo","volume":"92","author":"Kaufman","year":"2016","journal-title":"Neuron"},{"key":"2024032523420366700_nlab087-B27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40478-018-0637-7","article-title":"Different tau species lead to heterogeneous tau pathology propagation and misfolding","volume":"6","author":"Dujardin","year":"2018","journal-title":"Acta Neuropathol Comm"},{"key":"2024032523420366700_nlab087-B28","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1038\/ncb1901","article-title":"Transmission and spreading of tauopathy in transgenic mouse brain","volume":"11","author":"Clavaguera","year":"2009","journal-title":"Nat Cell Biol"},{"key":"2024032523420366700_nlab087-B29","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s00401-013-1231-5","article-title":"Peripheral administration of tau aggregates triggers intracerebral tauopathy in transgenic mice","volume":"127","author":"Clavaguera","year":"2014","journal-title":"Acta Neuropathol"},{"key":"2024032523420366700_nlab087-B30","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.1084\/jem.20160833","article-title":"Unique pathological tau conformers from Alzheimer\u2019s brains transmit tau pathology in nontransgenic mice","volume":"213","author":"Guo","year":"2016","journal-title":"J Exp Med"},{"key":"2024032523420366700_nlab087-B31","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s00401-015-1458-4","article-title":"Tau pathology spread in PS19 tau transgenic mice following locus coeruleus (LC) injections of synthetic tau fibrils is determined by the LC\u2019s afferent and efferent connections","volume":"130","author":"Iba","year":"2015","journal-title":"Acta Neuropathol"},{"key":"2024032523420366700_nlab087-B32","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s00401-014-1373-0","article-title":"Differential induction and spread of tau pathology in young PS19 tau transgenic mice following intracerebral injections of pathological tau from Alzheimer\u2019s disease or corticobasal degeneration brains","volume":"129","author":"Boluda","year":"2015","journal-title":"Acta Neuropathol"},{"key":"2024032523420366700_nlab087-B33","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1093\/jnen\/nlaa109","article-title":"Risk of transmissibility from neurodegenerative disease-associated proteins: Experimental knowns and unknowns","volume":"79","author":"Asher","year":"2020","journal-title":"J Neuropathol Exp Neurol"},{"key":"2024032523420366700_nlab087-B34","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1056\/NEJMc2000687","article-title":"Variant Creutzfeldt\u2013Jakob disease diagnosed 7.5 years after occupational exposure","volume":"383","author":"Brandel","year":"2020","journal-title":"N Engl J Med"},{"key":"2024032523420366700_nlab087-B35","doi-asserted-by":"crossref","first-page":"10788","DOI":"10.1038\/s41598-018-28856-2","article-title":"Assessment of the efficacy of different procedures that remove and disassemble alpha-synuclein, tau and A-beta fibrils from laboratory material and surfaces","volume":"8","author":"Fenyi","year":"2018","journal-title":"Sci Rep"},{"key":"2024032523420366700_nlab087-B36","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1186\/s40478-014-0151-5","article-title":"Decontamination of medical devices from pathological amyloid-\u03b2-, tau-and \u03b1-synuclein aggregates","volume":"2","author":"Thomzig","year":"2014","journal-title":"Acta Neuropathol Commun"},{"key":"2024032523420366700_nlab087-B37","first-page":"e53205","article-title":"Sensitive detection of proteopathic seeding activity with FRET flow cytometry","author":"Furman","year":"2015","journal-title":"J Vis Exp"},{"key":"2024032523420366700_nlab087-B38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jalz.2011.10.007","article-title":"National Institute on Aging\u2013Alzheimer\u2019s Association guidelines for the neuropathologic assessment of Alzheimer\u2019s disease","volume":"8","author":"Hyman","year":"2012","journal-title":"Alzheimers Dement"},{"key":"2024032523420366700_nlab087-B39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00401-011-0910-3","article-title":"National Institute on Aging\u2013Alzheimer\u2019s Association guidelines for the neuropathologic assessment of Alzheimer\u2019s disease: A practical approach","volume":"123","author":"Montine","year":"2012","journal-title":"Acta Neuropathol"},{"key":"2024032523420366700_nlab087-B40","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1093\/brain\/awy117","article-title":"Tau reduction in the presence of amyloid-\u03b2 prevents tau pathology and neuronal death in vivo","volume":"141","author":"DeVos","year":"2018","journal-title":"Brain"},{"key":"2024032523420366700_nlab087-B41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ncomms9490","article-title":"Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer\u2019s disease brain","volume":"6","author":"Takeda","year":"2015","journal-title":"Nature Commun"},{"key":"2024032523420366700_nlab087-B42","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/bs.mcb.2017.06.009","article-title":"The use of mouse models to study cell-to-cell transmission of pathological tau","volume":"141","author":"Narasimhan","year":"2017","journal-title":"Meth Cell Biol"},{"key":"2024032523420366700_nlab087-B43","doi-asserted-by":"crossref","first-page":"267","DOI":"10.3389\/fnins.2018.00267","article-title":"Synaptic tau seeding precedes tau pathology in human Alzheimer\u2019s disease brain","volume":"12","author":"DeVos","year":"2018","journal-title":"Front Neurosci"},{"key":"2024032523420366700_nlab087-B44","doi-asserted-by":"crossref","DOI":"10.1212\/WNL.40.6.887","article-title":"A simple and effective method for inactivating virus infectivity in formalin-fixed tissue samples from patients with Creutzfeldt-Jakob disease","author":"Brown","year":"1990","journal-title":"Neurology"},{"key":"2024032523420366700_nlab087-B45","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/978-1-4939-7244-9_22","article-title":"Methods for molecular diagnosis of human Prion disease","author":"Wadsworth","year":"2017","journal-title":"Prions"},{"key":"2024032523420366700_nlab087-B46","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1038\/s41586-020-2043-0","article-title":"Novel tau filament fold in corticobasal degeneration","volume":"580","author":"Zhang","year":"2020","journal-title":"Nature"},{"key":"2024032523420366700_nlab087-B47","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1126\/science.1113694","article-title":"Tau suppression in a neurodegenerative mouse model improves memory function","volume":"309","author":"Santacruz","year":"2005","journal-title":"Science"},{"key":"2024032523420366700_nlab087-B48","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1074\/jbc.M114.589309","article-title":"Conformation determines the seeding potencies of native and recombinant Tau aggregates","volume":"290","author":"Falcon","year":"2015","journal-title":"J Biol Chem"},{"key":"2024032523420366700_nlab087-B49","doi-asserted-by":"crossref","first-page":"3546","DOI":"10.1074\/jbc.M805627200","article-title":"Conformational diversity of wild-type Tau fibrils specified by templated conformation change","volume":"284","author":"Frost","year":"2009","journal-title":"J Biol Chem"},{"key":"2024032523420366700_nlab087-B50","doi-asserted-by":"crossref","first-page":"fcab096","DOI":"10.1093\/braincomms\/fcab096","article-title":"Kinetics of tau aggregation reveals patient specific tau characteristics among Alzheimer\u2019s cases","volume":"3","author":"Kamath","year":"2021","journal-title":"Brain Commun"},{"key":"2024032523420366700_nlab087-B51","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1016\/j.ajpath.2017.03.011","article-title":"Enhanced tau aggregation in the presence of amyloid \u03b2","volume":"187","author":"Bennett","year":"2017","journal-title":"Am J Pathol"},{"key":"2024032523420366700_nlab087-B52","doi-asserted-by":"crossref","first-page":"5827","DOI":"10.1073\/pnas.87.15.5827","article-title":"A preparation of Alzheimer paired helical filaments that displays distinct tau proteins by polyacrylamide gel electrophoresis","volume":"87","author":"Greenberg","year":"1990","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2024032523420366700_nlab087-B53","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1007\/978-1-61779-551-0_32","article-title":"Biochemical isolation of insoluble tau in transgenic mouse models of tauopathies","volume":"849","author":"Julien","year":"2012","journal-title":"Methods Mol Biol"},{"key":"2024032523420366700_nlab087-B54","doi-asserted-by":"crossref","first-page":"2709","DOI":"10.1016\/j.ajpath.2016.06.008","article-title":"High\u2013molecular-weight paired helical filaments from Alzheimer brain induces seeding of wild-type mouse tau into an Argyrophilic 4R tau pathology in vivo","volume":"186","author":"Audouard","year":"2016","journal-title":"Am J Pathol"},{"key":"2024032523420366700_nlab087-B55","doi-asserted-by":"crossref","first-page":"11485","DOI":"10.1523\/JNEUROSCI.2393-17.2017","article-title":"GFP-mutant human tau transgenic mice develop Tauopathy following CNS injections of Alzheimer\u2019s brain-derived pathological tau or synthetic mutant human tau fibrils","volume":"37","author":"Gibbons","year":"2017","journal-title":"J Neurosci"},{"key":"2024032523420366700_nlab087-B56","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40478-017-0442-8","article-title":"Characterization of tau prion seeding activity and strains from formaldehyde-fixed tissue","volume":"5","author":"Kaufman","year":"2017","journal-title":"Acta Neuropathol Comm"},{"key":"2024032523420366700_nlab087-B57","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/s00401-014-1339-2","article-title":"A\u03b2 seeds resist inactivation by formaldehyde","volume":"128","author":"Fritschi","year":"2014","journal-title":"Acta Neuropathol"},{"key":"2024032523420366700_nlab087-B58","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s00401-014-1360-5","article-title":"Formaldehyde-fixed brain tissue from spontaneously ill \u03b1-synuclein transgenic mice induces fatal \u03b1-synucleinopathy in transgenic hosts","volume":"129","author":"Schweighauser","year":"2015","journal-title":"Acta Neuropathol"},{"key":"2024032523420366700_nlab087-B59","doi-asserted-by":"crossref","first-page":"20309","DOI":"10.1074\/jbc.M611876200","article-title":"Fibrillogenic nuclei composed of P301L mutant tau induce elongation of P301L tau but not wild-type tau","volume":"282","author":"Aoyagi","year":"2007","journal-title":"J Biol Chem"},{"key":"2024032523420366700_nlab087-B60","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.sbi.2020.05.011","article-title":"Cryo-EM structures of tau filaments","volume":"64","author":"Scheres","year":"2020","journal-title":"Curr Opin Struct Biol"}],"container-title":["Journal of Neuropathology &amp; Experimental Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jnen\/article-pdf\/80\/10\/912\/40874846\/nlab087.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jnen\/article-pdf\/80\/10\/912\/40874846\/nlab087.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T00:13:31Z","timestamp":1711412011000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jnen\/article\/80\/10\/912\/6366482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,8]]},"references-count":60,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,9,8]]},"published-print":{"date-parts":[[2021,10,26]]}},"URL":"https:\/\/doi.org\/10.1093\/jnen\/nlab087","relation":{},"ISSN":["0022-3069","1554-6578"],"issn-type":[{"value":"0022-3069","type":"print"},{"value":"1554-6578","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,10,1]]},"published":{"date-parts":[[2021,9,8]]}}}