{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:44:22Z","timestamp":1761677062296,"version":"3.37.3"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Key Research Project","award":["2017YFA0104200"],"award-info":[{"award-number":["2017YFA0104200"]}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"publisher","award":["82001593","81971433","81971428"],"award-info":[{"award-number":["82001593","81971433","81971428"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Bureau of Sichuan Province","award":["2021YJ0017","2020YFS0041"],"award-info":[{"award-number":["2021YJ0017","2020YFS0041"]}]},{"name":"National Key Project of Neonatal Children","award":["1311200003303"],"award-info":[{"award-number":["1311200003303"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>miR-466b-5p is aberrantly upregulated in oligodendrocyte precursor cells (OPCs) after white matter injury (WMI). However, its roles in neonatal WMI pathogenesis are unknown. In this study, P3 rats were subjected to hypoxia-ischemia to establish a neonatal WMI model. A bioinformatic analysis was conducted to predict the possible target of miR-466b-5p as Lpar1. RT-PCR was performed to validate the expression of miR-466b-5p and Lpar1 mRNA. The miR-466b-5p antagomir was intracerebroventricularly administrated to inhibit miR-466b-5p; OPC differentiation, apoptosis, proliferation, and myelination were analyzed using immunofluorescence staining, western blotting, and electron microscopy. In addition, the behavioral performance of the rats was measured with the Morris water maze test. Sox10 expression and PLP trafficking were examined to elucidate the mechanism by which miR-466b-5p regulates WMI pathogenesis. We found that after inhibiting miR-466b-5p, the Edg2 protein was increased, OPC differentiation and myelinated axon formation were enhanced, and the rats\u2019 behavioral performance was improved, whereas OPC proliferation and apoptosis were not affected. Furthermore, the expression of Sox10 was promoted while PLP trafficking was attenuated after miR-466b-5p inhibition. We conclude that miR-466b-5p is involved in the regulation of WMI pathogenesis, partly through the Lpar1\/Edg2\/Sox10 and Lpar1\/Edg2\/PLP signaling pathways.<\/jats:p>","DOI":"10.1093\/jnen\/nlac012","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T13:48:32Z","timestamp":1646315312000},"page":"260-270","source":"Crossref","is-referenced-by-count":2,"title":["Inhibiting miR-466b-5p Attenuates Neonatal White Matter Injury by Targeting Lpar1"],"prefix":"10.1093","volume":"81","author":[{"given":"Dongqiong","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University From the , Chengdu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojuan","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University From the , Chengdu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyun","family":"Gou","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University From the , Chengdu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyi","family":"Huang","sequence":"additional","affiliation":[{"name":"West China College of Stomatology, Sichuan University , Chengdu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjie","family":"Ying","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University From the , Chengdu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University From the , Chengdu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University From the , Chengdu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dezhi","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and 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