{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:45:27Z","timestamp":1788371127936,"version":"build-2803163510"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Cerebral infarction (CI) is one of the leading causes of disability and death. LncRNAs are key factors in CI progression. Herein, we studied the function of long noncoding RNA KCNQ1OT1 in CI patient plasma samples and in CI models. Quantitative real-time PCR and Western blotting tested gene and protein expressions. The interactions of KCNQ1OT1\/PTBP1 and miR-16-5p were analyzed using dual-luciferase reporter and RNA immunoprecipitation assays; MTT assays measured cell viability. Cell migration and angiogenesis were tested by wound healing and tube formation assays. Pathological changes were analyzed by triphenyltetrazolium chloride and routine staining. We found that KCNQ1OT1 and PTBP1 were overexpressed and miR-16-5p was downregulated in CI patient plasma and in oxygen-glucose deprived (OGD) induced mouse brain microvascular endothelial (bEnd.3) cells. KCNQ1OT1 knockdown suppressed pro-inflammatory cytokine production and stimulated angiogenic responses in OGD-bEnd.3 cells. KCNQ1OT1 upregulated PTBP1 by sponging miR-16-5p. PTBP1 overexpression or miR-16-5p inhibition attenuated the effects of KCNQ1OT1 knockdown. PTBP1 silencing protected against OGD-bEnd.3 cell injury by enhancing SIRT1. KCNQ1OT1 silencing or miR-16-5p overexpression also alleviated ischemic injury in a mice middle cerebral artery occlusion model. Thus, KCNQ1OT1 silencing alleviates CI by regulating the miR-16-5p\/PTBP1\/SIRT1 pathway, providing a theoretical basis for novel therapeutic strategies targeting CI.<\/jats:p>","DOI":"10.1093\/jnen\/nlae005","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T20:37:21Z","timestamp":1707338241000},"page":"276-288","source":"Crossref","is-referenced-by-count":3,"title":["Long noncoding RNA KCNQ1OT1 aggravates cerebral infarction by regulating PTBT1\/SIRT1 via miR-16-5p"],"prefix":"10.1093","volume":"83","author":[{"given":"Yuanming","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiu","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fifth Affiliated Hospital of Harbin Medical University , Daqing, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanfei","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihang","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuobo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"key":"2024032009310673500_nlae005-B1","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1016\/j.jstrokecerebrovasdis.2017.06.023","article-title":"Isosteviol sodium protects against permanent cerebral ischemia injury in mice via inhibition of NF-kappaB-mediated inflammatory and apoptotic 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