{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T08:18:58Z","timestamp":1771834738304,"version":"3.50.1"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R28AA012725"],"award-info":[{"award-number":["R28AA012725"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alcohol use disorder (AUD) is characterized by an inability to stop consuming alcohol. Neuroimaging studies of patients with AUD show mild grey and white matter atrophy, while pathological studies suggest that atrophy is restricted to the white matter. The effects on individual brain cells are largely unknown. Mild neuronal loss has been described in the prefrontal cortex but this has not been consistent. Studies quantifying oligodendrocytes, astrocytes, and microglia are rare. These knowledge gaps impede therapeutic advancements. Here, we piloted the use of tissue microarrays, immunohistochemistry, and automated image analysis to systematically quantify cell profiles in human postmortem tissue. We sampled 173 grey and white matter cores across 5 cerebral regions from 4 male AUD cases and 4 age-matched controls. We found no obvious differences in the regional profiles of neurons, astrocytes, oligodendrocytes, or microglia. However, mean microglial densities across all regions were higher in AUD (P=.0024). There were visual signs of reactive astrocytosis in AUD cases but mean cell body sizes were unchanged. Our findings suggest that alcohol-related brain damage is not due to a loss of any of major cell classes. Larger studies focusing on subtype-specific markers and advanced image analysis tools are required.<\/jats:p>","DOI":"10.1093\/jnen\/nlaf147","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T13:04:45Z","timestamp":1764162285000},"page":"267-279","source":"Crossref","is-referenced-by-count":0,"title":["Quantification of the neuropathology of alcohol use disorder using tissue microarrays"],"prefix":"10.1093","volume":"85","author":[{"given":"Jie","family":"Liu","sequence":"first","affiliation":[{"name":"Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney , Camperdown, NSW,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew J","family":"Catanzariti","sequence":"additional","affiliation":[{"name":"Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney , Camperdown, NSW,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huang-Tuong","family":"Nguyen-Hao","sequence":"additional","affiliation":[{"name":"Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney , Camperdown, NSW,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Novelli","sequence":"additional","affiliation":[{"name":"Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney , Camperdown, NSW,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9448-7934","authenticated-orcid":false,"given":"Dhiraj","family":"Maskey","sequence":"additional","affiliation":[{"name":"Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney , Camperdown, NSW,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4476-390X","authenticated-orcid":false,"given":"Caine C","family":"Smith","sequence":"additional","affiliation":[{"name":"Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney , Camperdown, NSW,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9613-6188","authenticated-orcid":false,"given":"Julia C","family":"Stevens","sequence":"additional","affiliation":[{"name":"Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney , Camperdown, NSW,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5111-3978","authenticated-orcid":false,"given":"Markus J","family":"Hofer","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences and Charles Perkins Centre, Faculty of Science, The University of Sydney , Sydney, NSW,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5886-2306","authenticated-orcid":false,"given":"Suzanne M","family":"de la Monte","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Laboratory Medicine, Neurology, Neurosurgery, and Medicine, Rhode Island Hospital, Brown University Health, Alpert Medical School of Brown University, Providence, RI,","place":["United States"]},{"name":"Department of Medicine, Rhode Island Hospital, Lifespan Academic Institutions , and the Warren Alpert Medical School of Brown University, Providence, RI,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2493-9736","authenticated-orcid":false,"given":"Greg Trevor","family":"Sutherland","sequence":"additional","affiliation":[{"name":"Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney , Camperdown, NSW,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,12,23]]},"reference":[{"key":"2026022302520422900_nlaf147-B1","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/BF00294379","article-title":"Neuronal counts from four cortical regions of alcoholic brains","volume":"79","author":"Kril","year":"1989","journal-title":"Acta Neuropathol"},{"key":"2026022302520422900_nlaf147-B2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1111\/j.1530-0277.1996.tb01045.x","article-title":"Chronic alcoholics without Wernicke-Korsakoff syndrome or cirrhosis do not lose serotonergic neurons in the dorsal raphe nucleus","volume":"20","author":"Baker","year":"1996","journal-title":"Alcohol Clin Exp 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