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In this study, we performed a comparative evaluation of a recombinant rabbit monoclonal anti-pT217 antibody (mAb) using immunohistochemical and biochemical analyzes in human and transgenic mouse tauopathies and compared results to those of a commercially available anti-pT217 antibody and the AT8 antibody. In rTg4510 mice, the anti-pT217 mAb detected pathological tau aggregates with high sensitivity, including hyperphosphorylated tau species identified by biochemical fraction. In human postmortem brain tissues (AD, Pick disease, progressive supranuclear palsy, corticobasal degeneration), quantitative image analysis demonstrated labeling of a broad range of tau lesions, including neurofibrillary tangles, neuropil threads, tufted astrocytes, astrocytic plaques, and Pick bodies. The overall detectability was comparable to that of AT8 and similar to or modestly\u2009&amp;gt;\u2009that of a representative commercial anti-pT217 antibody. These findings indicate that pT217 is widely present across tauopathies and suggest that the recombinant anti-pT217 mAb provides a sensitive and reproducible tool for neuropathological assessment. Together, these results provide a framework for evaluating anti-pT217 antibodies in neuropathological studies.<\/jats:p>","DOI":"10.1093\/jnen\/nlag103","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:50:08Z","timestamp":1792626608000},"source":"Crossref","is-referenced-by-count":0,"title":["Comparative neuropathological evaluation of pT217 immunoreactivity in human and transgenic mouse tauopathies"],"prefix":"10.1093","author":[{"given":"Emi","family":"Higashiizumi","sequence":"first","affiliation":[{"name":"Department of Innovative Neurodiagnostic Technologies, Brain Research Institute, Niigata University , Niigata,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brain Research Institute, Niigata University , Niigata,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Kishida Chemical Co., Ltd ., Osaka,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Koyama","sequence":"additional","affiliation":[{"name":"Kishida Chemical Co., Ltd ., Osaka,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruki","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Cell Engineering Corporation , Osaka,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Nakatani","sequence":"additional","affiliation":[{"name":"Cell Engineering Corporation , Osaka,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Tachibana","sequence":"additional","affiliation":[{"name":"Cell Engineering Corporation , Osaka,","place":["Japan"]},{"name":"Department of Chemistry and Bioengineering, School of Engineering, Osaka Metropolitan University , Osaka,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa E","family":"Murray","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Mayo Clinic , Jacksonville, FL,","place":["United States"]},{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic , Jacksonville, FL,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis W","family":"Dickson","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Mayo Clinic , Jacksonville, FL,","place":["United States"]},{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic , Jacksonville, FL,","place":["United 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