{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T22:57:59Z","timestamp":1783637879174,"version":"3.55.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T00:00:00Z","timestamp":1566777600000},"content-version":"vor","delay-in-days":34,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Grant-in-Aid for Scientific Research","award":["17K07097"],"award-info":[{"award-number":["17K07097"]}]},{"name":"Grant-in-Aid for Scientific Research","award":["26290017"],"award-info":[{"award-number":["26290017"]}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Sciences","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Human prion diseases including sporadic Creutzfeldt-Jakob disease (sCJD), inherited prion diseases, and acquired human prion diseases are lethal neurodegenerative diseases. One of the major sources of iatrogenic Creutzfeldt-Jakob disease was human growth hormone (hGH-iCJD) derived from contaminated cadaveric pituitaries. The incidence of hGH-iCJD has decreased since changing from growth hormone extracted from human cadaveric pituitaries to recombinant pituitary hormones. However, extensive analysis on the localization and detecting of abnormal prion protein in the pituitary gland are limited. In this study, we examined 9 autopsied brains and pituitary glands from 6 patients with prion disease (3 Gerstmann-Str\u00e4ussler-Scheinker disease, 2 sCJD, and 1 dura mater graft-associated CJD) and 3 individuals with nonprion diseases. Western blot analysis of pituitary samples demonstrated unique glycoforms of normal cellular prion protein with molecular weights of 30\u201340\u2009kDa, which was higher than the typical 25\u201335\u2009kDa prion protein in brains. Proteomic analysis also revealed prion protein approximately the molecular weight of 40\u2009kDa in pituitary samples. Moreover, proteinase K-resistant Prion protein was frequently detected in pituitary samples of the prion diseases. Immunohistochemistry for Prion protein revealed mosaic cellular distribution preferentially in growth hormone- or prolactin-producing cells.<\/jats:p>","DOI":"10.1093\/jnen\/nlz075","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T11:45:52Z","timestamp":1566819952000},"page":"922-929","source":"Crossref","is-referenced-by-count":6,"title":["Frequent Detection of Pituitary-Derived PrPres in Human Prion Diseases"],"prefix":"10.1093","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4225-7248","authenticated-orcid":false,"given":"Hiroyuki","family":"Honda","sequence":"first","affiliation":[{"name":"Department of Neuropathology, Graduate School of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Division of Proteomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Shijo","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Graduate School of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideomi","family":"Hamasaki","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Graduate School of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoko","family":"Sadashima","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Graduate School of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi O","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Graduate School of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Aishima","sequence":"additional","affiliation":[{"name":"Department of Pathology, Saga University Hospital, Saga, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Kai","sequence":"additional","affiliation":[{"name":"Department of Pathology, Saga University Hospital, Saga, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiichi I","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology and Division of Proteomics, Medical Institute of Bioregulation, Kyushu University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naokazu","family":"Sasagasako","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neuro-Muscular Center, National Omuta Hospital, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Iwaki","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Graduate School of Medical 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