{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T04:42:15Z","timestamp":1788410535535,"version":"build-2803163510"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T00:00:00Z","timestamp":1641772800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The FilmArray Meningitis\/Encephalitis panel (MEP) has an 11% false-positive and 2.2% false-negative rate compared with conventional testing. We describe clinical characteristics, treatment decisions, and outcomes in children with discordant results between MEP and conventional testing.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We conducted a multisite review of patients \u2264 18 years with suspected central nervous system infection and positive results by MEP or conventional testing (cerebrospinal fluid [CSF] culture, herpes simplex virus [HSV] polymerase chain reaction (PCR), and enterovirus [EV] PCR). Descriptive results are provided for patients with discordant results. Comparison between group 1 (MEP and CSF culture positive) and group 2 (MEP positive, CSF culture negative, or showing a different pathogen) was made by Mann-Whitney test for continuous and Fisher\u2019s test for categorical variables.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A total of 355 patients had at least one pathogen identified. More than half of patients with bacterial pathogens identified that are included in the MEP had discordant results (30\/52; 58%). There were 28 samples with bacterial pathogen identified on MEP only, 1 with different bacterial pathogens on MEP and culture, and 1 with Escherichia coli identified on CSF culture only. Patients in group 1 were more likely to have CSF pleocytosis, elevated CSF protein, and decreased CSF glucose than group 2 (P &amp;lt; .05). Two patients were HSV positive by MEP while HSV negative by PCR. Ten patients had discordant results between MEP and EV PCR.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Discordant results between MEP and conventional testing are common. Treatment decisions based on a positive MEP should be made in the appropriate clinical context.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpids\/piab126","type":"journal-article","created":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T12:14:04Z","timestamp":1637842444000},"page":"134-141","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of Discordant Results Between FilmArray Meningitis\/Encephalitis Panel and Conventional Testing in Pediatric Patients: A Multisite Retrospective Cohort Study"],"prefix":"10.1093","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7973-9953","authenticated-orcid":false,"given":"Maheswari","family":"Ekambaram","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Baylor Scott and White Medical Center, Round Rock, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleisha","family":"Nabower","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Nebraska Medical Center, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prabi","family":"Rajbhandari","sequence":"additional","affiliation":[{"name":"Division of Hospital Medicine, Department of Pediatrics, Akron Children\u2019s Hospital, Akron, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaclyn","family":"Eisenberg","sequence":"additional","affiliation":[{"name":"Section of Pediatric Hospital Medicine, Department of Pediatrics, University of Chicago Medical Center, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel","family":"Goodrich","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Nebraska Medical Center, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krow","family":"Ampofo","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan S","family":"Gollehon","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Nebraska Medical Center, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly C","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Oklahoma School of Community Medicine, Tulsa, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Lyden","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, University of Nebraska College of Public Health, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0715-151X","authenticated-orcid":false,"given":"Jessica","family":"Snowden","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Arkansas Medical Center, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,1,10]]},"reference":[{"key":"2022043012125586000_CIT0001","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/S1473-3099(14)70805-9","article-title":"Epidemiology of bacterial meningitis in the USA from 1997 to 2010: a population-based observational study.","volume":"14","author":"Castelblanco","year":"2014","journal-title":"Lancet Infect Dis"},{"key":"2022043012125586000_CIT0002","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1086\/425368","article-title":"Practice guidelines for the management of bacterial meningitis.","volume":"39","author":"Tunkel","year":"2004","journal-title":"Clin Infect Dis"},{"key":"2022043012125586000_CIT0003","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.clp.2014.10.004","article-title":"Bacterial meningitis in infants.","volume":"42","author":"Ku","year":"2015","journal-title":"Clin Perinatol"},{"key":"2022043012125586000_CIT0004","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1542\/peds.2005-1132","article-title":"Neonatal meningitis: what is the correlation among cerebrospinal fluid cultures, blood cultures, and cerebrospinal fluid parameters?","volume":"117","author":"Garges","year":"2006","journal-title":"Pediatrics"},{"key":"2022043012125586000_CIT0005","volume-title":"FDA Allows Marketing of the First Nucleic Acid-Based Test to Detect Multiple Pathogens from CSF. 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