{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T10:45:43Z","timestamp":1785321943147,"version":"3.55.0"},"reference-count":88,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"European Union\u2019s Horizon 2020"},{"name":"Marie Sk\u0142odowska-Curie","award":["872370"],"award-info":[{"award-number":["872370"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The main objective of the present study was to develop a nanoparticulate drug delivery system that can protect insulin against harsh conditions in the gastrointestinal (GI) tract. The effects of the following employed techniques, including lyophilisation, cross-linking and nanoencapsulation, on the physicochemical properties of the formulation were investigated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We herein developed a nanocarrier via ionotropic gelation by using positively charged chitosan and negatively charged Dz13Scr. The lyophilised nanoparticles with optimal concentrations of tripolyphosphate (cross-linking agent) and \u03b2-cyclodextrin (stabilising agent) were characterised by using physical and cellular assays.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>The addition of cryoprotectants (1% sucrose) in lyophilisation improved the stability of nanoparticles, enhanced the encapsulation efficiency, and ameliorated the pre-mature release of insulin at acidic pH. The developed lyophilised nanoparticles did not display any cytotoxic effects in C2C12 and HT-29 cells. Glucose consumption assays showed that the bioactivity of entrapped insulin was maintained post-incubation in the enzymatic medium.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Freeze-drying with appropriate cryoprotectant could conserve the physiochemical properties of the nanoparticles. The bioactivity of the entrapped insulin was maintained. The prepared nanoparticles could facilitate the permeation of insulin across the GI cell line.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgaa052","type":"journal-article","created":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T04:13:53Z","timestamp":1609474433000},"page":"726-739","source":"Crossref","is-referenced-by-count":13,"title":["\u03b2-Cyclodextrin-containing chitosan-oligonucleotide nanoparticles improve insulin bioactivity, gut cellular permeation and glucose consumption"],"prefix":"10.1093","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6132-6432","authenticated-orcid":false,"given":"Chun Yuen Jerry","family":"Wong","sequence":"first","affiliation":[{"name":"School of Pharmacy and Biomedical Sciences, Curtin University , Bentley,","place":["Australia"]},{"name":"Curtin Health Innovation Research Institute , Bentley,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0049-6969","authenticated-orcid":false,"given":"Hani","family":"Al-Salami","sequence":"additional","affiliation":[{"name":"Biotechnology and Drug Development Research Laboratory, School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute , Bentley,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crispin R","family":"Dass","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Biomedical Sciences, Curtin University , Bentley,","place":["Australia"]},{"name":"Curtin Health Innovation Research Institute , Bentley,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,6,1]]},"reference":[{"key":"2024103012060675200_CIT0001","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.ijbiomac.2013.03.064","article-title":"Oral delivery of insulin from alginate\/chitosan crosslinked by glutaraldehyde","volume":"58","author":"Tahtat","year":"2013","journal-title":"Int J Biol Macromol"},{"key":"2024103012060675200_CIT0002","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.ijpharm.2015.10.078","article-title":"Goblet cell targeting nanoparticle containing drug-loaded micelle cores for oral delivery of insulin","volume":"496","author":"Zhang","year":"2015","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0003","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1208\/s12249-016-0594-z","article-title":"Eudragit S100-coated chitosan nanoparticles co-loading tat for enhanced oral colon absorption of insulin","volume":"18","author":"Chen","year":"2017","journal-title":"AAPS Pharmscitech"},{"key":"2024103012060675200_CIT0004","first-page":"1","article-title":"Fabrication techniques for the preparation of orally administered insulin nanoparticles","author":"Wong","year":"2021","journal-title":"J Drug Target"},{"key":"2024103012060675200_CIT0005","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1080\/03639045.2020.1788061","article-title":"Development of orally administered insulin-loaded polymeric-oligonucleotide nanoparticles: statistical optimization and physicochemical characterization","volume":"46","author":"Wong","year":"2020","journal-title":"Drug Dev Ind Pharm"},{"key":"2024103012060675200_CIT0006","doi-asserted-by":"crossref","first-page":"5127","DOI":"10.2147\/IJN.S161240","article-title":"Synthesis and characterization of a novel peptide-grafted Cs and evaluation of its nanoparticles for the oral delivery of insulin, in vitro, and in vivo study","volume":"13","author":"Barbari","year":"2018","journal-title":"Int J Nanomed"},{"key":"2024103012060675200_CIT0007","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1080\/1061186X.2020.1759078","article-title":"Current status and applications of animal models in pre-clinical development of orally administered insulin-loaded nanoparticles","volume":"28","author":"Wong","year":"2020","journal-title":"J Drug Target"},{"key":"2024103012060675200_CIT0008","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1080\/1061186X.2020.1726356","article-title":"Cellular assays and applied technologies for characterisation of orally administered protein nanoparticles: a systematic review","volume":"28","author":"Wong","year":"2020","journal-title":"J Drug Target"},{"key":"2024103012060675200_CIT0009","doi-asserted-by":"crossref","first-page":"118720","DOI":"10.1016\/j.ijpharm.2019.118720","article-title":"Bio-nanotechnological advancement of orally administered insulin nanoparticles: Comprehensive review of experimental design for physicochemical characterization","volume":"572","author":"Wong","year":"2019","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0010","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.ijpharm.2015.05.065","article-title":"Design, characterisation, and bioefficiency of insulin-chitosan nanoparticles after stabilisation by freeze-drying or cross-linking","volume":"491","author":"Diop","year":"2015","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0011","doi-asserted-by":"crossref","first-page":"9678","DOI":"10.1016\/j.biomaterials.2013.08.048","article-title":"Intestinal mucosa permeability following oral insulin delivery using core shell corona nanolipoparticles","volume":"34","author":"Li","year":"2013","journal-title":"Biomaterials"},{"key":"2024103012060675200_CIT0012","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.nano.2009.04.007","article-title":"Preparation and characterization of insulin nanoparticles using chitosan and Arabic gum with ionic gelation method","volume":"6","author":"Avadi","year":"2010","journal-title":"Nanomedicine"},{"key":"2024103012060675200_CIT0013","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1021\/acsnano.5b00028","article-title":"Overcoming the diffusion barrier of mucus and absorption barrier of epithelium by self-assembled nanoparticles for oral delivery of insulin","volume":"9","author":"Shan","year":"2015","journal-title":"ACS Nano"},{"key":"2024103012060675200_CIT0014","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1039\/C7TB02450A","article-title":"Iron-mimic peptide converts transferrin from foe to friend for orally targeting insulin delivery","volume":"6","author":"Liu","year":"2018","journal-title":"J Mater Chem B"},{"key":"2024103012060675200_CIT0015","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.ijpharm.2007.05.015","article-title":"Peroral delivery of insulin using chitosan derivatives: a comparative study of polyelectrolyte nanocomplexes and nanoparticles","volume":"342","author":"Jintapattanakit","year":"2007","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0016","doi-asserted-by":"crossref","first-page":"155","DOI":"10.18433\/J3J88Q","article-title":"Efficient peroral delivery of insulin via vitamin B12 modified trimethyl chitosan nanoparticles","volume":"18","author":"Ke","year":"2015","journal-title":"J Pharm Pharm Sci"},{"key":"2024103012060675200_CIT0017","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.ejps.2010.08.009","article-title":"Pharmacological effect of orally delivered insulin facilitated by multilayered stable nanoparticles","volume":"41","author":"Woitiski","year":"2010","journal-title":"Eur J Pharm Sci"},{"key":"2024103012060675200_CIT0018","doi-asserted-by":"crossref","first-page":"2902","DOI":"10.1039\/C8FO00238J","article-title":"Fabrication and characterization of complex nanoparticles based on carboxymethyl short chain amylose and chitosan by ionic gelation","volume":"9","author":"Ji","year":"2018","journal-title":"Food Funct"},{"key":"2024103012060675200_CIT0019","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.ijpharm.2006.06.019","article-title":"Cyclodextrin-insulin complex encapsulated polymethacrylic acid based nanoparticles for oral insulin delivery","volume":"325","author":"Sajeesh","year":"2006","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0020","first-page":"860109","article-title":"Ex vivo evaluation of insulin nanoparticles using chitosan and arabic gum","volume":"2011","author":"Avadi","year":"2011","journal-title":"ISRN Pharm"},{"key":"2024103012060675200_CIT0021","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.ijpharm.2018.07.041","article-title":"Recent advancements in oral administration of insulin-loaded liposomal drug delivery systems for diabetes mellitus","volume":"549","author":"Wong","year":"2018","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0022","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.ijpharm.2017.12.036","article-title":"Microparticles, microcapsules and microspheres: a review of recent developments and prospects for oral delivery of insulin","volume":"537","author":"Wong","year":"2018","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0023","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1111\/jphp.12607","article-title":"Oral delivery of insulin for treatment of diabetes: status quo, challenges and opportunities","volume":"68","author":"Wong","year":"2016","journal-title":"J Pharm Pharmacol"},{"key":"2024103012060675200_CIT0024","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.jconrel.2017.09.003","article-title":"Potential of insulin nanoparticle formulations for oral delivery and diabetes treatment","volume":"264","author":"Wong","year":"2017","journal-title":"J Control Release"},{"key":"2024103012060675200_CIT0025","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/jphp.12694","article-title":"In-vitro evaluation of enteric coated insulin tablets containing absorption enhancer and enzyme inhibitor","volume":"69","author":"Wong","year":"2017","journal-title":"J Pharm Pharmacol"},{"key":"2024103012060675200_CIT0026","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1080\/02652040802033673","article-title":"Biocompatible chitosan-DNAzyme nanoparticle exhibits enhanced biological activity","volume":"25","author":"Dass","year":"2008","journal-title":"J Microencapsul"},{"key":"2024103012060675200_CIT0027","doi-asserted-by":"crossref","first-page":"6991","DOI":"10.1007\/s00216-018-1319-9","article-title":"Quantification of BSA-loaded chitosan\/oligonucleotide nanoparticles using reverse-phase high-performance liquid chromatography","volume":"410","author":"Wong","year":"2018","journal-title":"Anal Bioanal Chem"},{"key":"2024103012060675200_CIT0028","doi-asserted-by":"crossref","first-page":"2058","DOI":"10.3390\/molecules23082058","article-title":"Aqueous-methanol extracts of orange-fleshed sweet potato (Ipomoeabatatas) ameliorate oxidative stress and modulate type 2 diabetes associated genes in insulin resistant C2C12 cells","volume":"23","author":"Ayeleso","year":"2018","journal-title":"Molecules"},{"key":"2024103012060675200_CIT0029","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1002\/cbin.10004","article-title":"High glucose-mediated alterations of mechanisms important in myogenesis of mouse C2C12 myoblasts","volume":"37","author":"Grzelkowska-Kowalczyk","year":"2013","journal-title":"Cell Biol Int"},{"key":"2024103012060675200_CIT0030","doi-asserted-by":"crossref","first-page":"2329","DOI":"10.1016\/j.biomaterials.2008.12.066","article-title":"In vivo evaluation of safety and efficacy of self-assembled nanoparticles for oral insulin delivery","volume":"30","author":"Sonaje","year":"2009","journal-title":"Biomaterials"},{"key":"2024103012060675200_CIT0031","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.carbpol.2014.04.048","article-title":"Decanoic acid grafted oligochitosan nanoparticles as a carrier for insulin transport in the gastrointestinal tract","volume":"111","author":"Du","year":"2014","journal-title":"Carbohydr Polym"},{"key":"2024103012060675200_CIT0032","doi-asserted-by":"crossref","first-page":"2801","DOI":"10.1016\/j.biomaterials.2011.12.038","article-title":"Protease inhibition and absorption enhancement by functional nanoparticles for effective oral insulin delivery","volume":"33","author":"Su","year":"2012","journal-title":"Biomaterials"},{"key":"2024103012060675200_CIT0033","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1021\/mp400493b","article-title":"Penetratin derivative-based nanocomplexes for enhanced intestinal insulin delivery","volume":"11","author":"Zhu","year":"2014","journal-title":"Mol Pharm"},{"key":"2024103012060675200_CIT0034","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.ijbiomac.2017.03.181","article-title":"Systematic investigation of fabrication conditions of nanocarrier based on carboxymethyl chitosan for sustained release of insulin","volume":"102","author":"Bai","year":"2017","journal-title":"Int J Biol Macromol"},{"key":"2024103012060675200_CIT0035","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.ijpharm.2016.01.022","article-title":"Insulin loaded mucus permeating nanoparticles: Addressing the surface characteristics as feature to improve mucus permeation","volume":"500","author":"de Sousa","year":"2016","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0036","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jconrel.2015.12.008","article-title":"Efficient mucus permeation and tight junction opening by dissociable \u201cmucus-inert\u201d agent coated trimethyl chitosan nanoparticles for oral insulin delivery","volume":"222","author":"Liu","year":"2016","journal-title":"J Control Release"},{"key":"2024103012060675200_CIT0037","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.jconrel.2012.11.011","article-title":"Calcium depletion-mediated protease inhibition and apical-junctional-complex disassembly via an EGTA-conjugated carrier for oral insulin delivery","volume":"169","author":"Chuang","year":"2013","journal-title":"J Control Release"},{"key":"2024103012060675200_CIT0038","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.jconrel.2013.05.006","article-title":"Noninvasive imaging oral absorption of insulin delivered by nanoparticles and its stimulated glucose utilization in controlling postprandial hyperglycemia during OGTT in diabetic rats","volume":"172","author":"Chuang","year":"2013","journal-title":"J Control Release"},{"key":"2024103012060675200_CIT0039","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.ijpharm.2010.04.006","article-title":"Effective protection and controlled release of insulin by cationic beta-cyclodextrin polymers from alginate\/chitosan nanoparticles","volume":"393","author":"Zhang","year":"2010","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0040","doi-asserted-by":"crossref","first-page":"3384","DOI":"10.1016\/j.biomaterials.2010.01.042","article-title":"Enteric-coated capsules filled with freeze-dried chitosan\/poly(gamma-glutamic acid) nanoparticles for oral insulin delivery","volume":"31","author":"Sonaje","year":"2010","journal-title":"Biomaterials"},{"key":"2024103012060675200_CIT0041","doi-asserted-by":"crossref","first-page":"6849","DOI":"10.1016\/j.biomaterials.2010.05.042","article-title":"Biodistribution, pharmacodynamics and pharmacokinetics of insulin analogues in a rat model: Oral delivery using pH-responsive nanoparticles vs. subcutaneous injection","volume":"31","author":"Sonaje","year":"2010","journal-title":"Biomaterials"},{"key":"2024103012060675200_CIT0042","doi-asserted-by":"crossref","first-page":"3695","DOI":"10.1002\/adfm.201001014","article-title":"Self-assembled pH-sensitive nanoparticles: a platform for oral delivery of protein drugs","volume":"20","author":"Sonaje","year":"2010","journal-title":"Adv Funct Mater"},{"key":"2024103012060675200_CIT0043","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ejpb.2009.06.002","article-title":"Design for optimization of nanoparticles integrating biomaterials for orally dosed insulin","volume":"73","author":"Woitiski","year":"2009","journal-title":"Eur J Pharm Biopharm"},{"key":"2024103012060675200_CIT0044","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1016\/j.actbio.2009.03.007","article-title":"Colloidal carrier integrating biomaterials for oral insulin delivery: Influence of component formulation on physicochemical and biological parameters","volume":"5","author":"Woitiski","year":"2009","journal-title":"Acta Biomater"},{"key":"2024103012060675200_CIT0045","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1021\/bm0607776","article-title":"Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery","volume":"8","author":"Lin","year":"2007","journal-title":"Biomacromolecules"},{"key":"2024103012060675200_CIT0046","doi-asserted-by":"crossref","first-page":"105102","DOI":"10.1088\/0957-4484\/18\/10\/105102","article-title":"Novel nanoparticles for oral insulin delivery via the paracellular pathway","volume":"18","author":"Lin","year":"2007","journal-title":"Nanotechnology"},{"key":"2024103012060675200_CIT0047","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1021\/bc800076n","article-title":"Oral delivery of peptide drugs using nanoparticles self-assembled by poly(gamma-glutamic acid) and a chitosan derivative functionalized by trimethylation","volume":"19","author":"Mi","year":"2008","journal-title":"Bioconjug Chem"},{"key":"2024103012060675200_CIT0048","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1166\/jnn.2007.609","article-title":"Insulin-loaded nanoparticles are prepared by alginate ionotropic pre-gelation followed by chitosan polyelectrolyte complexation","volume":"7","author":"Sarmento","year":"2007","journal-title":"J Nanosci Nanotechnol"},{"key":"2024103012060675200_CIT0049","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.ejpb.2006.09.005","article-title":"Probing insulin\u2019s secondary structure after entrapment into alginate\/chitosan nanoparticles","volume":"65","author":"Sarmento","year":"2007","journal-title":"Eur J Pharm Biopharm"},{"key":"2024103012060675200_CIT0050","doi-asserted-by":"crossref","first-page":"2198","DOI":"10.1007\/s11095-007-9367-4","article-title":"Alginate\/chitosan nanoparticles are effective for oral insulin delivery","volume":"24","author":"Sarmento","year":"2007","journal-title":"Pharm Res"},{"key":"2024103012060675200_CIT0051","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.carbpol.2006.02.008","article-title":"Characterization of insulin-loaded alginate nanoparticles produced by ionotropic pre-gelation through DSC and FTIR studies","volume":"66","author":"Sarmento","year":"2006","journal-title":"Carbohydr Polym"},{"key":"2024103012060675200_CIT0052","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.ijpharm.2004.12.025","article-title":"Pharmacological activity of peroral chitosan-insulin nanoparticles in diabetic rats","volume":"293","author":"Ma","year":"2005","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0053","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1002\/jps.10149","article-title":"Formulation pH modulates the interaction of insulin with chitosan nanoparticles","volume":"91","author":"Ma","year":"2002","journal-title":"J Pharm Sci"},{"key":"2024103012060675200_CIT0054","first-page":"1051","article-title":"Relationship between drug effects and particle size of insulin-loaded bioadhesive microspheres","volume":"23","author":"Pan","year":"2002","journal-title":"Acta Pharmacol Sin"},{"key":"2024103012060675200_CIT0055","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0378-5173(02)00486-6","article-title":"Bioadhesive polysaccharide in protein delivery system: chitosan nanoparticles improve the intestinal absorption of insulin in vivo","volume":"249","author":"Pan","year":"2002","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0056","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijpharm.2011.12.055","article-title":"HP55-coated capsule containing PLGA\/RS nanoparticles for oral delivery of insulin","volume":"425","author":"Wu","year":"2012","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0057","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.jconrel.2018.07.045","article-title":"Physicochemical, pharmacokinetic and pharmacodynamic analyses of amphiphilic cyclodextrin-based nanoparticles designed to enhance intestinal delivery of insulin","volume":"286","author":"Presas","year":"2018","journal-title":"J Control Release"},{"key":"2024103012060675200_CIT0058","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.ijbiomac.2018.01.077","article-title":"A cell-penetrating peptide conjugated carboxymethyl-\u03b2-cyclodextrin to improve intestinal absorption of insulin","volume":"111","author":"Yang","year":"2018","journal-title":"Int J Biol Macromol"},{"key":"2024103012060675200_CIT0059","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1161\/ATVBAHA.109.189753","article-title":"c-Jun DNAzymes inhibit myocardial inflammation, ROS generation, infarct size, and improve cardiac function after ischemia-reperfusion injury","volume":"29","author":"Luo","year":"2009","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"2024103012060675200_CIT0060","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.ejps.2010.12.007","article-title":"Design and evaluation of novel pH-sensitive chitosan nanoparticles for oral insulin delivery","volume":"42","author":"Makhlof","year":"2011","journal-title":"Eur J Pharm Sci"},{"key":"2024103012060675200_CIT0061","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1080\/09205063.2017.1301774","article-title":"Multiboronic acid-conjugated chitosan scaffolds with glucose selectivity to insulin release","volume":"28","author":"Siddiqui","year":"2017","journal-title":"J Biomater Sci Polym Ed"},{"key":"2024103012060675200_CIT0062","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1208\/s12249-020-01648-6","article-title":"Lyophilisation improves bioactivity and stability of insulin-loaded polymeric-oligonucleotide nanoparticles for diabetes treatment","volume":"21","author":"Wong","year":"2020","journal-title":"AAPS Pharmscitech"},{"key":"2024103012060675200_CIT0063","doi-asserted-by":"crossref","first-page":"101738","DOI":"10.1016\/j.jddst.2020.101738","article-title":"Formulation and characterisation of insulin-loaded chitosan nanoparticles capable of inducing glucose uptake in skeletal muscle cells in vitro","volume":"57","author":"Wong","year":"2020","journal-title":"J Drug Deliv Sci Technol"},{"key":"2024103012060675200_CIT0064","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1002\/jps.20520","article-title":"Self-assembled polyelectrolyte nanocomplexes between chitosan derivatives and insulin","volume":"95","author":"Mao","year":"2006","journal-title":"J Pharm Sci"},{"key":"2024103012060675200_CIT0065","first-page":"5865","article-title":"Probing insulin bioactivity in oral nanoparticles produced by ultrasonication-assisted emulsification\/internal gelation","volume":"10","author":"Lopes","year":"2015","journal-title":"Int J Nanomed"},{"key":"2024103012060675200_CIT0066","doi-asserted-by":"crossref","first-page":"3489","DOI":"10.2147\/IJN.S81715","article-title":"pH-sensitive poly(lactide-co-glycolide) nanoparticle composite microcapsules for oral delivery of insulin","volume":"10","author":"Sun","year":"2015","journal-title":"Int J Nanomed"},{"key":"2024103012060675200_CIT0067","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1002\/mabi.201300515","article-title":"Thiolated eudragit-based nanoparticles for oral insulin delivery: Preparation, characterization, and evaluation using intestinal epithelial cells in vitro","volume":"14","author":"Zhang","year":"2014","journal-title":"Macromol Biosci"},{"key":"2024103012060675200_CIT0068","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1007\/s11051-012-1219-4","article-title":"Concanavalin A conjugated biodegradable nanoparticles for oral insulin delivery","volume":"14","author":"Hurkat","year":"2012","journal-title":"J Nanopart Res"},{"key":"2024103012060675200_CIT0069","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1111\/jphp.13359","article-title":"C2C12 cell model: its role in understanding of insulin resistance at the molecular level and pharmaceutical development at the preclinical stage","volume":"72","author":"Wong","year":"2020","journal-title":"J Pharm Pharmacol"},{"key":"2024103012060675200_CIT0070","doi-asserted-by":"crossref","first-page":"3369","DOI":"10.1021\/acs.molpharmaceut.8b00391","article-title":"Alginate-C18 conjugate nanoparticles loaded in tripolyphosphate-cross-linked chitosan-oleic acid conjugate-coated calcium alginate beads as oral insulin carrier","volume":"15","author":"Alfatama","year":"2018","journal-title":"Mol Pharm"},{"key":"2024103012060675200_CIT0071","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1021\/mp400685v","article-title":"Mechanism study of cellular uptake and tight junction opening mediated by goblet cell-specific trimethyl chitosan nanoparticles","volume":"11","author":"Zhang","year":"2014","journal-title":"Mol Pharm"},{"key":"2024103012060675200_CIT0072","doi-asserted-by":"crossref","first-page":"e1801010","DOI":"10.1002\/adhm.201801010","article-title":"Scalable production of therapeutic protein nanoparticles using flash nanoprecipitation","volume":"8","author":"Zeng","year":"2019","journal-title":"Adv Healthc Mater"},{"key":"2024103012060675200_CIT0073","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/17425247.2018.1420050","article-title":"Development and characterization of lipid-polymeric nanoparticles for oral insulin delivery","volume":"15","author":"Sgorla","year":"2018","journal-title":"Expert Opin Drug Deliv"},{"key":"2024103012060675200_CIT0074","doi-asserted-by":"crossref","first-page":"34039","DOI":"10.1021\/acsami.8b13707","article-title":"Multifunctional nanoparticles enable efficient oral delivery of biomacromolecules via improving payload stability and regulating the transcytosis pathway","volume":"10","author":"Zheng","year":"2018","journal-title":"ACS Appl Mater Interfaces"},{"key":"2024103012060675200_CIT0075","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.ijpharm.2016.03.011","article-title":"Annealing as a tool for the optimization of lyophilization and ensuring of the stability of protein-loaded PLGA nanoparticles","volume":"503","author":"Fonte","year":"2016","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0076","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.ijpharm.2014.07.049","article-title":"Preparation and characterization of PEG-coated silica nanoparticles for oral insulin delivery","volume":"473","author":"Andreani","year":"2014","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0077","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1208\/s12249-011-9716-9","article-title":"Preparation, statistical optimization, and in vitro characterization of insulin nanoparticles composed of quaternized aromatic derivatives of chitosan","volume":"12","author":"Mahjub","year":"2011","journal-title":"AAPS Pharmscitech"},{"key":"2024103012060675200_CIT0078","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1016\/j.ijbiomac.2018.11.147","article-title":"New glucose oxidase-immobilized stimuli-responsive dextran nanoparticles for insulin delivery","volume":"123","author":"Jamwal","year":"2019","journal-title":"Int J Biol Macromol"},{"key":"2024103012060675200_CIT0079","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1002\/(SICI)1097-4628(20000228)75:9<1089::AID-APP1>3.0.CO;2-5","article-title":"Beta cyclodextrin-insulin-encapsulated chitosan\/alginate matrix: oral delivery system","volume":"75","author":"Rowsen Moses","year":"2000","journal-title":"J Appl Polym Sci"},{"key":"2024103012060675200_CIT0080","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.3109\/03639045.2013.841187","article-title":"Lyophilized insulin nanoparticles prepared from quaternized N-aryl derivatives of chitosan as a new strategy for oral delivery of insulin: in vitro, ex vivo and in vivo characterizations","volume":"40","author":"Mahjub","year":"2014","journal-title":"Drug Dev Ind Pharm"},{"key":"2024103012060675200_CIT0081","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.2217\/nnm.12.31","article-title":"Folate-decorated PLGA nanoparticles as a rationally designed vehicle for the oral delivery of insulin","volume":"7","author":"Jain","year":"2012","journal-title":"Nanomedicine (Lond)"},{"key":"2024103012060675200_CIT0082","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.ijpharm.2009.09.018","article-title":"The influence of polymer architecture on the protective effect of novel comb shaped amphiphilic poly(allylamine) against in vitro enzymatic degradation of insulin\u2013towards oral insulin delivery","volume":"383","author":"Thompson","year":"2010","journal-title":"Int J Pharm"},{"key":"2024103012060675200_CIT0083","doi-asserted-by":"crossref","first-page":"3552","DOI":"10.1080\/10717544.2016.1209796","article-title":"pH responsive cylindrical MSN for oral delivery of insulin-design, fabrication and evaluation","volume":"23","author":"Guha","year":"2016","journal-title":"Drug Deliv"},{"key":"2024103012060675200_CIT0084","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1021\/acsbiomaterials.8b00646","article-title":"Dual stimuli-responsive nanoparticle-incorporated hydrogels as an oral insulin carrier for intestine-targeted delivery and enhanced paracellular permeation","volume":"4","author":"Liu","year":"2018","journal-title":"ACS Biomater Sci Eng"},{"key":"2024103012060675200_CIT0085","doi-asserted-by":"crossref","first-page":"9916","DOI":"10.1021\/acsami.7b16524","article-title":"Biomimetic viruslike and charge reversible nanoparticles to sequentially overcome mucus and epithelial barriers for oral insulin delivery","volume":"10","author":"Wu","year":"2018","journal-title":"ACS Appl Mater Interfaces"},{"key":"2024103012060675200_CIT0086","doi-asserted-by":"crossref","first-page":"e1800285","DOI":"10.1002\/adhm.201800285","article-title":"Uniform core-shell nanoparticles with thiolated hyaluronic acid coating to enhance oral delivery of insulin","volume":"7","author":"Tian","year":"2018","journal-title":"Adv Healthc Mater"},{"key":"2024103012060675200_CIT0087","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1080\/1061186X.2017.1400552","article-title":"The role of chitosan on oral delivery of peptide-loaded nanoparticle formulation","volume":"26","author":"Wong","year":"2018","journal-title":"J Drug Target"},{"key":"2024103012060675200_CIT0088","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.jconrel.2006.12.003","article-title":"A novel vitamin B12-nanosphere conjugate carrier system for peroral delivery of insulin","volume":"117","author":"Chalasani","year":"2007","journal-title":"J Control Release"}],"container-title":["Journal of Pharmacy and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/73\/6\/726\/60217569\/rgaa052.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/73\/6\/726\/60217569\/rgaa052.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T16:48:30Z","timestamp":1730306910000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jpp\/article\/73\/6\/726\/6188957"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,1]]},"references-count":88,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6,1]]},"published-print":{"date-parts":[[2021,6,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jpp\/rgaa052","relation":{},"ISSN":["0022-3573","2042-7158"],"issn-type":[{"value":"0022-3573","type":"print"},{"value":"2042-7158","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,6,1]]},"published":{"date-parts":[[2021,6,1]]}}}