{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T19:30:27Z","timestamp":1788550227610,"version":"build-2803163510"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Immunotherapy using recombinant monoclonal antibodies specifically Anti-amyloid-beta (Anti-A\u03b2) scFv is envisaged as an appropriate therapeutic for Alzheimer through reduction of amyloid-beta aggregation. The solubilization of therapeutics using polymeric micelles facilitates an improved bioavailability and extended blood half-life. In this study, the optimum production condition for Anti-amyloid-beta (Anti-A\u03b2) scFv was obtained. To increase the stability of plasma, Anti-A\u03b2-loaded polymeric micelles were synthesized.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Escherichia coli SHuffle expression strain was used and purified by Ni-NTA. Pluronics P85 and F127 micelles were used for the Anti-A\u03b2 delivery and were characterized in terms of morphology, drug loading and drug release in phosphate buffer and artificial cerebrospinal fluid. The stability profile was quantified at 4\u00b0C over a 30 days storage period. The stability in human plasma was also evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>Proteins expressed in SHuffle resulted in increased levels of protein expression and solubility. Low critical micelle concentration value and high micelle encapsulation efficiency (&amp;lt;200 nm) achieved via direct dissolution method. Anti-A\u03b2-loaded micelles were around 2.2-fold more stable than Anti-A\u03b2 in plasma solution. A sustained in-vitro release of Anti-A\u03b2 from micelles was observed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Results confirmed that Pluronic-micelles pose benefits as a nano-carrier to increase the stability of Anti-A\u03b2 scFvin in the plasma.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgaa068","type":"journal-article","created":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T04:13:53Z","timestamp":1609474433000},"page":"460-472","source":"Crossref","is-referenced-by-count":9,"title":["Anti-A\u03b2-scFv-loaded polymeric nano-micelles with enhanced plasma stability"],"prefix":"10.1093","volume":"73","author":[{"given":"Farnaz","family":"Sotoudegan","sequence":"first","affiliation":[{"name":"Biotechnology Research Center, Pasteur Institute of Iran , Tehran,","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzaneh","family":"Sotoudegan","sequence":"additional","affiliation":[{"name":"Pharmaceutical Quality Assurance Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences , Tehran,","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeganeh","family":"Talebkhan Garoosi","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Pasteur Institute of Iran , Tehran,","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar H","family":"Afshar","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy International Campus, Tehran University of Medical Sciences , Tehran,","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzaneh","family":"Barkhordari","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Pasteur Institute of Iran , Tehran,","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7693-2926","authenticated-orcid":false,"given":"Fatemeh","family":"Davami","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Pasteur Institute of Iran , Tehran,","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,4,1]]},"reference":[{"key":"2024103012055343800_CIT0001","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1126\/science.1072994","article-title":"The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics","volume":"297","author":"Hardy","year":"2002","journal-title":"Science"},{"issue":"3 Suppl","key":"2024103012055343800_CIT0002","doi-asserted-by":"crossref","first-page":"151","DOI":"10.3233\/JAD-2006-9S317","article-title":"Alzheimer's disease: the amyloid cascade hypothesis: an update and reappraisal","volume":"9","author":"Hardy","year":"2006","journal-title":"J Alzheimers Dis"},{"key":"2024103012055343800_CIT0003","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1111\/j.1471-4159.2006.04426.x","article-title":"A beta oligomers - a decade of discovery","volume":"101","author":"Walsh","year":"2007","journal-title":"J Neurochem"},{"key":"2024103012055343800_CIT0004","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/nm0303-269","article-title":"Immunotherapy: past, present and future","volume":"9","author":"Waldmann","year":"2003","journal-title":"Nat Med"},{"key":"2024103012055343800_CIT0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fnagi.2014.00114","article-title":"Immunotherapy for Alzheimer's disease: past, present and future","volume":"6","author":"Spencer","year":"2014","journal-title":"Front Aging Neurosci"},{"key":"2024103012055343800_CIT0006","doi-asserted-by":"crossref","first-page":"6093","DOI":"10.1093\/hmg\/ddv321","article-title":"Anti-A\u03b2 single-chain variable fragment antibodies exert synergistic neuroprotective activities in Drosophila models of Alzheimer's disease","volume":"24","author":"Fernandez-Funez","year":"2015","journal-title":"Hum Mol Genet"},{"key":"2024103012055343800_CIT0007","doi-asserted-by":"crossref","first-page":"114","DOI":"10.3389\/fnagi.2014.00114","article-title":"Immunotherapy for Alzheimer's disease: past, present and future","volume":"6","author":"Spencer","year":"2014","journal-title":"Front Aging Neurosci"},{"key":"2024103012055343800_CIT0008","first-page":"7","volume-title":"The blood-brain barrier. 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