{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:11:17Z","timestamp":1762272677060,"version":"3.37.3"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Science and Technology Planning Project of Guangdong Province of China","award":["2015B020211006"],"award-info":[{"award-number":["2015B020211006"]}]},{"name":"Shenzhen Foundation of Science and Technology","award":["JCYJ20190814112205770"],"award-info":[{"award-number":["JCYJ20190814112205770"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81603373"],"award-info":[{"award-number":["81603373"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The aim of the study was to explore the effect of total glucosides of paeony (TGP) and Tripterygium wilfordii polyglycosides (TWP) on erythrocyte methotrexate polyglutamates (MTXPGs), the metabolites of methotrexate (MTX).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>An ultra-high-performance liquid chromatography (UPLC)-tandem mass spectrometry (MS\/MS) method was developed to determine MTXPGs. The effects of MTXPGs were analysed using 24 male Sprague-Dawley rats that were randomly divided into the MTX alone, MTX-TGP combined, and MTX-TWP combined groups. Rats were administered MTX at a dose of 0.9 mg\/kg once a week, TGP at 0.054 g\/kg and TWP at 1.8 mg\/kg three times a day. Venous blood (1.0 ml) was collected at weeks 2, 4, 6, 9, 12 and 15 and then analysed using the developed UPLC-MS\/MS method.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>Specificity, linear range, inter-and intra-day precision, recovery, matrix effect and stability of MTXPGs met the standard regulations. This method was successfully used for the detection of MTXPGs. After administration of MTX alone, erythrocyte MTXPGs increased and accumulated in a time- and dose-dependent manner. Compared to MTX alone, the combination with TGP significantly decreased the content of total MTXPGs and short-chain MTXPGs (Methotrexate [MTX\/MTXPG1] and 4-amino-10-methylpteroyldiglutamic acid [MTXPG2], P &amp;lt; 0.05), but had no significant effect on long-chain MTXPGs (4-amino-10-methylpteroyltriglutamic acid [MTXPG3], P &amp;gt; 0.05) and very long-chain MTXPGs (4-amino-10-methylpteroyltetraglutamic acid [MTXPG4] and 4-amino-10-methylpteroylpentaglutamic acid [MTXPG5], P &amp;gt; 0.05) at week 15. The combination of MTX with TWP had no significant effect on the content of total MTXPGs, short-chain MTXPGs and long-chain MTXPGs (P &amp;gt; 0.05), but it significantly decreased the content of very long-chain MTXPGs (P &amp;lt; 0.05) at week 15.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The UPLC-MS\/MS method was successfully used to determine MTXPGs in rat erythrocytes. TGP and TWP in combination with MTX affected the production of MTXPGs of different chain lengths in erythrocytes.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgab025","type":"journal-article","created":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T15:46:45Z","timestamp":1612540005000},"page":"1039-1048","source":"Crossref","is-referenced-by-count":3,"title":["Effect of total glucosides of paeony and <i>Tripterygium wilfordii<\/i> polyglycosides on erythrocyte methotrexate polyglutamates in rats, analysed using ultra-high-performance liquid chromatography-tandem mass spectrometry"],"prefix":"10.1093","volume":"73","author":[{"given":"Shulong","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Shenzhen Hospital, Southern Medical University , Shenzhen, Guangdong ,","place":["China"]},{"name":"School of Traditional Chinese Medicine, Southern Medical University , Guangzhou, Guangdong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqian","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Nanfang Hospital, Southern Medical University , Guangzhou, Guangdong,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunxiao","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shenzhen Hospital, Southern Medical University , Shenzhen, Guangdong ,","place":["China"]},{"name":"School of Traditional Chinese Medicine, Southern Medical University , Guangzhou, Guangdong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianrong","family":"Xun","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shenzhen Hospital, Southern Medical University , Shenzhen, Guangdong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaokang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shenzhen Hospital, Southern Medical University , Shenzhen, Guangdong ,","place":["China"]},{"name":"School of Traditional Chinese Medicine, Southern Medical University , Guangzhou, Guangdong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Nanfang Hospital, Southern Medical University , Guangzhou, Guangdong,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shenzhen Hospital, Southern Medical University , Shenzhen, Guangdong ,","place":["China"]},{"name":"School of Traditional Chinese Medicine, Southern Medical University , Guangzhou, Guangdong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Southern Medical University , Guangzhou, Guangdong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shenzhen Hospital, Southern Medical University , Shenzhen, Guangdong ,","place":["China"]},{"name":"School of Traditional Chinese Medicine, Southern Medical University , Guangzhou, Guangdong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Peng","sequence":"additional","affiliation":[{"name":"Hangzhou Biozon Medical Lab Co Ltd. , Hangzhou, Zhejiang ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Hangzhou Biozon 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