{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T18:33:21Z","timestamp":1791657201059,"version":"4.3.4"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81773934"],"award-info":[{"award-number":["81773934"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>This study aimed to clarify the superior beneficial effects of icariin on atherosclerosis, as well as to explore the possible underlying mechanisms for its effect via the modulation of protein kinase C \u03b2I.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Lipid profiles were determined while dissected aortas were prepared of ApoE\u2212\/\u2212 mice. The expression of protein kinase C \u03b2I and phosphorylation of protein kinase C \u03b2I were determined by immunohistochemistry analysis. Human vascular smooth muscle cells were subjected to ox-LDL stimulation. MTS assay was conducted to detect cell proliferation. A transwell migration assay was performed to evaluate migration capacity. Flow cytometric analysis was used to determine cell cycle progression. Quantitative real-time PCR and western blot were performed to assess gene expression.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Icariin significantly alleviated atherogenesis, as well as protein levels of protein kinase C \u03b2I and phosphorylated protein kinase C \u03b2I in the aorta. Icariin effectively suppressed cell proliferation and migration. protein kinase C \u03b2I, cyclin D1 and matrix metalloproteinase-9 were modulated in response to treatment with icariin. Protein kinase C activator reversed the protective effect of icariin on human vascular smooth muscle cells against ox- low-density lipoprotein, protein kinase C \u03b2 inhibitor augmented the inhibitory effect of icariin.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our findings highlight the probable application of icariin in atherosclerotic therapy and reveal that protein kinase C \u03b2I acts as a crucial regulator in the anti-atherosclerotic action of icariin.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgab040","type":"journal-article","created":{"date-parts":[[2021,2,16]],"date-time":"2021-02-16T21:29:50Z","timestamp":1613510990000},"page":"1169-1179","source":"Crossref","is-referenced-by-count":8,"title":["Essential role of protein kinase C \u03b2I in icariin-mediated protection against atherosclerosis"],"prefix":"10.1093","volume":"73","author":[{"given":"Yibing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, The First Hospital of Jilin University, Jilin University , Changchun ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongsheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Cancer Center, The First Hospital of Jilin University, Jilin University , Changchun ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University , Changchun ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University , Changchun ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University , Changchun ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery, The First Hospital of Jilin University, Jilin University , Changchun ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6939-3266","authenticated-orcid":false,"given":"Liqun","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University , Changchun 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