{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T10:04:58Z","timestamp":1787652298450,"version":"build-2736575974"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Central Council for Research in Unani Medicine","award":["F.No.3-145\/2017-CCRUM\/Tech"],"award-info":[{"award-number":["F.No.3-145\/2017-CCRUM\/Tech"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Limited treatment options are available for advanced stages of chronic myeloid leukaemia (CML). Moreover, patients\u2019 relapse after a short remission period, which prompts them to identify a potent drug with the least toxicity. An Unani herbal formulation, Itrifal-e-Aftimoon (IEA) is used for certain neurological disorders, however, its antitumor potential has not been reported yet in any malignancy, including CML.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The aqueous extract of IEA was characterized by HPLC\/LC-MS and used alone or in combination with standard drug, imatinib in CML cell lines (K562, KU812) in vitro to assess its effect on cancer-associated parameters such as cytotoxicity, cell cycle, apoptosis, oxidative stress, inflammation, angiogenesis, and certain signalling pathways.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>LC\u2013MS characterization of IEA showed the presence of antitumor compounds including catechin and caffeic acid. Treatment with IEA caused cytotoxicity and arrested cells in the sub-G0\/G1 phase. Subsequent assays confirmed apoptosis-mediated cell death with mitochondrial membrane depolarization and alleviation of oxidative stress. IEA abrogates IL-6, VEGF, angiopoietin-2, and alters Th1\/Th2 cytokines. IEA potentiated the effect of imatinib even at lower doses by affecting FAK\/STAT\/Akt\/ERK pathways.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>IEA possesses antitumor potential against CML and increases the efficacy of imatinib when used in combination, suggesting utilization of IEA as an adjuvant therapy for better management of CML in the future.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgac045","type":"journal-article","created":{"date-parts":[[2022,7,9]],"date-time":"2022-07-09T10:07:17Z","timestamp":1657361237000},"page":"1330-1341","source":"Crossref","is-referenced-by-count":4,"title":["Itrifal-e-Aftimoon potentiates imatinib-induced anti-leukemic effect by influencing FAK\/STAT\/Akt\/ERK signalling pathways against chronic myeloid leukaemia <i>in vitro<\/i>"],"prefix":"10.1093","volume":"74","author":[{"given":"Nidhi","family":"Gupta","sequence":"first","affiliation":[{"name":"Department of Biochemistry, All India Institute of Medical Sciences (AIIMS) , New Delhi ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Nafees","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, All India Institute of Medical Sciences (AIIMS) , New Delhi ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aziz Ur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Ilmul Saidla, Aligarh Muslim University , Aligarh ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamal","family":"Akhtar","sequence":"additional","affiliation":[{"name":"Central Council for Research in Unani Medicine , New Delhi ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asim 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