{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T13:44:00Z","timestamp":1771854240598,"version":"3.50.1"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>This study aimed to explore whether the liver-targeting enhancing effect of vinegar-baked Radix Bupleuri (VBRB) on rhein was achieved by affecting transporters, metabolism enzymes as well as hepatocyte nuclear factor 1\u03b1\/4\u03b1 (HNF1\u03b1\/HNF4\u03b1) in liver injury.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The effect of VBRB on the efficacy of rhein was performed with the LPS-induced acute liver injury rat model. Aspartate aminotransferase (AST), alanine transaminase (ALT) and superoxide dismutase (SOD) levels were determined and histopathological examination was taken. Drug concentrations in tissues were determined by high performance liquid chromatography (HPLC). The protein expressions of drug transporters, metabolic enzymes and hepatic nuclear factors were determined by Western blotting and ELISA assays.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key finding<\/jats:title>\n                  <jats:p>VBRB improved the liver protecting effect of rhein, which was consistent with its promoting effect on targeted enrichment of rhein in the liver. VBRB or in combination with rhein inhibited P-glycoprotein (Pgp) and multi-resistance related protein 2 (MRP2), while increased organic anion transporting polypeptide 2 (OATP2), which might be the reason why VBRB promoted liver-targeting effect of rhein.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>VBRB enhances the liver-protecting effect of rhein by down-regulating Pgp, MRP2, and up-regulating OATP2.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgac062","type":"journal-article","created":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T22:49:18Z","timestamp":1664664558000},"page":"1588-1597","source":"Crossref","is-referenced-by-count":13,"title":["Vinegar-baked Radix Bupleuri enhances the liver-targeting effect of rhein on liver injury rats by regulating transporters"],"prefix":"10.1093","volume":"74","author":[{"given":"Ya","family":"Zhao","sequence":"first","affiliation":[{"name":"The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Neihuan Xilu, Guangzhou Higher Education Mega Center , Guangzhou 510006 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinqiu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Guangdong Pharmaceutical University, Waihuan Donglu, Guangzhou Higher Education Mega Center , Guangzhou 510006 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Liu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Neihuan Xilu, Guangzhou Higher Education Mega Center , Guangzhou 510006 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yayun","family":"Wu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Neihuan Xilu, Guangzhou Higher Education Mega Center , Guangzhou 510006 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaohong","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Guangdong Pharmaceutical University, Waihuan Donglu, Guangzhou Higher Education Mega Center , Guangzhou 510006 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9554-3897","authenticated-orcid":false,"given":"Ruizhi","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Neihuan Xilu, Guangzhou Higher Education Mega Center , Guangzhou 510006 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