{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:48:17Z","timestamp":1792453697357,"version":"4.5.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2022,10,7]],"date-time":"2022-10-07T00:00:00Z","timestamp":1665100800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ulster University Vice-Chancellor Research Studentship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The antimalarial drug artemether is suggested to effect pancreatic islet cell transdifferentiation, presumably through activation \u03b3-aminobutyric acid receptors, but this biological action is contested.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We have investigated changes in \u03b1-cell lineage in response to 10-days treatment with artemether (100 mg\/kg oral, once daily) on a background of \u03b2-cell stress induced by multiple low-dose streptozotocin (STZ) injection in GluCreERT2; ROSA26-eYFP transgenic mice.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>Artemether intervention did not affect the actions of STZ on body weight, food and fluid intake or blood glucose. Circulating insulin and glucagon were reduced by STZ treatment, with a corresponding decline in pancreatic insulin content, which were not altered by artemether. The detrimental changes to pancreatic islet morphology induced by STZ were also evident in artemether-treated mice. Tracing of \u03b1-cell lineage, through co-staining for glucagon and yellow fluorescent protein (YFP), revealed a significant decrease of the proportion of glucagon+YFP\u2212 cells in STZ-diabetic mice, which was reversed by artemether. However, artemether had no effect on transdifferentiation of \u03b1-cells into \u03b2-cells and failed to augment the number of bi-hormonal, insulin+glucagon+, islet cells.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our observations confirm that artemisinin derivatives do not impart meaningful benefits on islet cell lineage transition events or pancreatic islet morphology.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgac075","type":"journal-article","created":{"date-parts":[[2022,10,7]],"date-time":"2022-10-07T16:55:11Z","timestamp":1665161711000},"page":"1758-1764","source":"Crossref","is-referenced-by-count":10,"title":["Effects of artemether on pancreatic islet morphology, islet cell turnover and \u03b1-cell transdifferentiation in insulin-deficient <i>Glu<\/i>\n                  <i>CreERT2<\/i>;<i>ROSA26-eYFP<\/i> diabetic mice"],"prefix":"10.1093","volume":"74","author":[{"given":"Dipak","family":"Sarnobat","sequence":"first","affiliation":[{"name":"Biomedical Sciences Research Institute, Centre for Diabetes, Ulster University , Coleraine ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan A","family":"Lafferty","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Research Institute, Centre for Diabetes, Ulster University , Coleraine ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Charlotte Moffett","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Research Institute, Centre for Diabetes, Ulster University , Coleraine ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei I","family":"Tarasov","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Research Institute, Centre for Diabetes, Ulster University , Coleraine ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter R","family":"Flatt","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Research Institute, Centre for Diabetes, Ulster University , Coleraine ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4855-964X","authenticated-orcid":false,"given":"Nigel","family":"Irwin","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Research Institute, Centre for Diabetes, Ulster University , Coleraine ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,10,7]]},"reference":[{"key":"2024103012193507500_CIT0001","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S2213-8587(20)30022-X","article-title":"Inadequate \u03b2-cell mass is essential for the pathogenesis of type 2 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