{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:56:49Z","timestamp":1792173409716,"version":"4.4.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:00:00Z","timestamp":1672963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To develop a sustained release 5-fluorouracil (5-FU) implant by three-dimensional (3D) printing to effectively prevent conjunctival fibrosis after glaucoma surgery.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>3D-printed implants composed of polycaprolactone (PCL) and chitosan (CS) were fabricated by heat extrusion technology and loaded with 1% 5-FU. Light microscopy and scanning electron microscopy were used to study the surface morphology. The 5-FU concentration released over 8 weeks was measured by ultraviolet visible spectroscopy. The effects on cell viability, fibroblast contractility and the expression of key fibrotic genes were assessed in human conjunctival fibroblasts.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>The PCL\u2013CS-5-FU implant sustainably released 5-FU over 8 weeks and the peak concentration was over 6.1 \u03bcg\/ml during weeks 1 and 2. The implant had a smooth surface and its total weight decreased by 3.5% after 8 weeks. The PCL\u2013CS\u20135-FU implant did not affect cell viability in conjunctival fibroblasts and sustainably suppressed fibroblast contractility and key fibrotic genes for 8 weeks.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The PCL\u2013CS\u20135-FU implant was biocompatible and degradable with a significant effect in suppressing fibroblast contractility. The PCL\u2013CS\u20135-FU implant could be used as a sustained release drug implant, replacing the need for repeated 5-FU injections in clinic, to prevent conjunctival fibrosis after glaucoma surgery.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgac100","type":"journal-article","created":{"date-parts":[[2023,1,8]],"date-time":"2023-01-08T06:14:08Z","timestamp":1673158448000},"page":"276-286","source":"Crossref","is-referenced-by-count":18,"title":["3D-printed long-acting 5-fluorouracil implant to prevent conjunctival fibrosis in glaucoma"],"prefix":"10.1093","volume":"75","author":[{"given":"Nicole","family":"Ioannou","sequence":"first","affiliation":[{"name":"Faculty of Life Sciences & Medicine, King\u2019s College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyuan","family":"Luo","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences & Medicine, King\u2019s College London , London ,","place":["UK"]},{"name":"Department of Ophthalmology, Renmin Hospital of Wuhan University , Wuhan ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqi","family":"Qin","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences & Medicine, King\u2019s College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Di Luca","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Queen\u2019s University Belfast , Belfast ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Essyrose","family":"Mathew","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Queen\u2019s University Belfast , Belfast ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aristides D","family":"Tagalakis","sequence":"additional","affiliation":[{"name":"Department of Biology, Edge Hill University , Ormskirk ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8740-1661","authenticated-orcid":false,"given":"Dimitrios A","family":"Lamprou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Queen\u2019s University Belfast , Belfast ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4868-5187","authenticated-orcid":false,"given":"Cynthia","family":"Yu-Wai-Man","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences & Medicine, King\u2019s College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,2,1]]},"reference":[{"key":"2024103012212794800_CIT0001","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1016\/j.ophtha.2014.05.013","article-title":"Global prevalence of glaucoma and projections of glaucoma burden through 2040: a systematic review and meta-analysis","volume":"121","author":"Tham","year":"2014","journal-title":"Ophthalmology"},{"key":"2024103012212794800_CIT0002","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1001\/archopht.1995.01100070092031","article-title":"Risk factors for open-angle glaucoma. 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