{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:19:30Z","timestamp":1758403170467,"version":"3.37.3"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673564","81102382"],"award-info":[{"award-number":["81673564","81102382"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shannxi Provincial Natural Science","award":["2019JM-121"],"award-info":[{"award-number":["2019JM-121"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>This study focuses on investigating the potential impact of DSPE-PEG polymers on intestinal absorption and related mechanism of berberine in rats.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Effect of DSPE-PEG polymer on intestinal absorption of berberine was investigated with an in situ closed-loop method in rats. To confirm the safety of DSPE-PEG polymer, morphological observation of rat intestine and measurement of biological markers in the intestinal perfusion of rats was performed. Underling mechanism behind promoting action of DSPE-PEG polymer was explored from its impact on the P-gp function and tight junction using in vitro diffusion chamber system, Caco-2 monolayer cells and western blot.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>DSPE-PEG polymer demonstrated significant enhancement action on the berberine absorption in rats without any obvious membrane toxicity. DSPE-PEG polymer (1.0%, w\/v) induced the most significant promoting effect on berberine absorption specifically in the small intestine of rats. Results of mechanistic studies revealed that DSPE-PEG polymer might not regulate intestinal P-gp function, but significantly down-regulated the expression of tight junction-related proteins, which accordingly led to loosening the tight junctions of intestinal epithelium cells, and consequently increased paracellular absorption of berberine in rats.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>DSPE-PEG polymer, as an excellent absorption enhancer, seems very promising in increasing oral bioavailability of berberine.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgad028","type":"journal-article","created":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T09:48:41Z","timestamp":1683971321000},"page":"931-939","source":"Crossref","is-referenced-by-count":5,"title":["DSPE-PEG polymer enhanced berberine absorption specifically in the small intestine of rats through paracellular passway"],"prefix":"10.1093","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4005-8085","authenticated-orcid":false,"given":"Yang","family":"Gao","sequence":"first","affiliation":[{"name":"School of Pharmacy, Xi\u2019an Jiaotong University , Xi\u2019an ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianmei","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xi\u2019an Jiaotong University , Xi\u2019an ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shucong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xi\u2019an Jiaotong University , Xi\u2019an ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisong","family":"Huxiao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xi\u2019an Jiaotong University , Xi\u2019an 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