{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:46:04Z","timestamp":1782859564442,"version":"3.54.5"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81891012"],"award-info":[{"award-number":["81891012"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U19A2010"],"award-info":[{"award-number":["U19A2010"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Interdisciplinary Innovation Team of Traditional Chinese Medicine","award":["ZYYCXTD-D-202209"],"award-info":[{"award-number":["ZYYCXTD-D-202209"]}]},{"name":"Sichuan TCM Science and Technology Industry Innovation","award":["2022C001"],"award-info":[{"award-number":["2022C001"]}]},{"name":"Xinglin Scholar Research Promotion Project of Chengdu University","award":["MPRC2022024"],"award-info":[{"award-number":["MPRC2022024"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students","award":["S202210633015"],"award-info":[{"award-number":["S202210633015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Safflower is a traditional Chinese medicine for the treatment of gynecological diseases and its flavonoids have potential anti-inflammatory effects. The purpose is to explore the possible effects of safflower total flavonoids (STF) on lipopolysaccharide (LPS)-induced inflammatory damage of Ishikawa cells.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Method<\/jats:title>\n                  <jats:p>In this study, LPS-induced endometrial carcinoma Ishikawa cells were used to establish an inflammatory injury model. The effective concentration of STF was screened by CCK-8 and enzyme-linked immunosorbent assay. The apoptosis of damaged Ishikawa cells was detected by flow cytometry. The contents of caspase11 and caspase 3 in Ishikawa cells were observed by fluorescence imaging. Western blot and RT-qPCR were used to detect the expression of related proteins and mRNA in damaged Ishikawa cells, and the possible mechanism of safflower flavonoids against LPS-induced endometrial carcinoma Ishikawa cells was analyzed by cell transcriptomics.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>The STF-reduced tumor necrosis factor \u03b1, interleukin-1\u03b2, and interleukin-6 expression level; the expression level of the proteins ASK1, Caspase3, and Caspase11 was also significantly decreased, and the proteins ER\u03b1, p-PI3K, and p-AKT were significantly increased. The transcriptome results showed that the PI3K-Akt signal pathway may be the main signal pathway for the STF.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The STF could regulate the PI3K\/AKT signal pathway to treat the inflammatory injury of Ishikawa cells.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgad082","type":"journal-article","created":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T17:48:48Z","timestamp":1695404928000},"page":"1478-1491","source":"Crossref","is-referenced-by-count":7,"title":["Explore the anti-inflammation mechanism of safflower total flavonoids in the treatment of endometritis based on celluar transcriptomics"],"prefix":"10.1093","volume":"75","author":[{"given":"Yan","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Southwestern Chinese Medicine Resources , Chengdu, Sichuan ,","place":["China"]},{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , Chengdu, Sichuan ,","place":["China"]},{"name":"College of Pharmacy, Chengdu University of Traditional Chinese Medicine , Chengdu, Sichuan ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwen","family":"Xiang","sequence":"additional","affiliation":[{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , Chengdu, Sichuan ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Southwestern Chinese Medicine Resources , Chengdu, Sichuan ,","place":["China"]},{"name":"School of Pharmacy, West China School of Pharmacy, Sichuan University , Chengdu ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , Chengdu, Sichuan ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , Chengdu, Sichuan ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyao","family":"Zeng","sequence":"additional","affiliation":[{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , Chengdu, Sichuan ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9892-1518","authenticated-orcid":false,"given":"Xiaofang","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Southwestern Chinese Medicine Resources , Chengdu, Sichuan ,","place":["China"]},{"name":"College of Pharmacy, Chengdu University of Traditional Chinese Medicine , Chengdu, Sichuan ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Southwestern Chinese Medicine Resources , Chengdu, Sichuan ,","place":["China"]},{"name":"College of Pharmacy, Chengdu University of Traditional Chinese Medicine , Chengdu, Sichuan ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,11,1]]},"reference":[{"key":"2024103012282055500_CIT0001","first-page":"167","article-title":"Raloxifene HCl is not stimulatory in the endometrium as assessed by the blaustein criteria and an estrogenicity scoring system","volume-title":"Prim Care Update Ob Gyns","author":"Shah","year":"1998"},{"key":"2024103012282055500_CIT0002","first-page":"1311","article-title":"Developmental biology of uterine glands","volume-title":"Biol Reprod","author":"Gray","year":"2001"},{"key":"2024103012282055500_CIT0003","first-page":"e8868","article-title":"Activation of the epithelial sodium channel (ENaC) leads to cytokine profile shift to pro-inflammatory in labor","volume-title":"EMBO Mol Med","author":"Sun","year":"2018"},{"key":"2024103012282055500_CIT0004","first-page":"1508","article-title":"Saikosaponin A inhibits LPS-induced endometritis in mice through activating Nrf2 signaling pathway","volume-title":"Inflammation","author":"Wang","year":"2018"},{"key":"2024103012282055500_CIT0005","first-page":"2111","article-title":"Anti-inflammatory cytokines in endometriosis","volume-title":"Cell Mol Life Sci","author":"Zhou","year":"2019"},{"key":"2024103012282055500_CIT0006","first-page":"6522","article-title":"Baicalin alleviates endometrial inflammatory injury through regulation of tight junction proteins","volume-title":"Food Funct","author":"Yang","year":"2022"},{"key":"2024103012282055500_CIT0007","first-page":"6672","article-title":"Medical uses of Carthamus tinctorius L. 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