{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T10:20:54Z","timestamp":1778494854320,"version":"3.51.4"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21375209"],"award-info":[{"award-number":["21375209"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Coptisine (Cop), an alkaloid isolated from Rhizoma Coptidis, has a protective effect against central nervous system diseases such as cerebral ischaemia-reperfusion (IR). Dysregulations in fatty acids metabolism are associated with neuroprotection and neuroinflammation. However, the effect of Cop on fatty acids metabolomics during anti-IR remains unclear.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Cerebral IR rats were established by middle cerebral artery occlusion, and the therapeutic effect of Cop was evaluated by 2, 3, 5-triphenytetrazolium chloride staining and neurological deficits scores. By liquid chromatography-tandem mass spectrometry (LC\u2013MS\/MS), fatty acids metabolomics analysis in ischaemic hemisphere and serum were investigated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We observed Cop (2 mg\/kg\/qd) was able to reduce cerebral infarct size and ameliorate the neurological function score. Meanwhile decrease in tumour necrosis factor-\u03b1 (TNF-\u03b1), and interleukin-1\u03b2 (IL-1\u03b2) after Cop treatment. Compared with control, down-regulation of cyclopentenone PGs (e.g., PGA2, PGJ2, and 15-deoxy- delta-12,14-PGJ2) was observed in cerebral IR, but upregulation of them when followed by Cop treatment. Similarly, we found the ratios of 14,15-dihydroxyeicosatrienoic acid(14,15-DHET)\/arachidonic acid and 11,12-DHET\/arachidonic acid was lower in cerebral IR injury relative to control, while their ratios were increased after Cop treatment.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Our results indicated that Cop protect against cerebral IR injury, and its mechanism might be closely associated with antiinflammation and the regulation of arachidonic acid metabolism<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jpp\/rgae026","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T17:17:44Z","timestamp":1711127864000},"page":"724-735","source":"Crossref","is-referenced-by-count":3,"title":["Coptisine protects against transient focal cerebral ischaemic injury by regulation of arachidonic acid metabolism"],"prefix":"10.1093","volume":"76","author":[{"given":"Junjie","family":"Zhang","sequence":"first","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory , Zhanjiang, Guangdong 524023 ,","place":["China"]},{"name":"School of Pharmacy, Guangdong Medical University , Zhanjiang, Guangdong 524023 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ao","family":"Qi","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory , Zhanjiang, Guangdong 524023 ,","place":["China"]},{"name":"School of Pharmacy, Guangdong Medical University , Zhanjiang, Guangdong 524023 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Shen","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory , Zhanjiang, Guangdong 524023 ,","place":["China"]},{"name":"School of Pharmacy, Guangdong Medical University , Zhanjiang, Guangdong 524023 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lulu","family":"Liu","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory , Zhanjiang, Guangdong 524023 ,","place":["China"]},{"name":"School of Pharmacy, Guangdong Medical University , Zhanjiang, Guangdong 524023 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0629-3337","authenticated-orcid":false,"given":"Chun","family":"Cai","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory , Zhanjiang, Guangdong 524023 ,","place":["China"]},{"name":"School of Pharmacy, Guangdong Medical University , Zhanjiang, Guangdong 524023 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan (Guangzhou University of Chinese Medicine), Ministry of Education, Research Center of Chinese Herbal Resource Science and Engineering, School of Traditional Chinese Meteria Medica, Guangzhou University of Chinese Medicine , Guangzhou, Guangdong 51006 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,6,1]]},"reference":[{"key":"2024103012043984500_CIT0001","first-page":"e56","article-title":"Heart Disease and Stroke Statistics-2019 Update: a report from the American Heart Association","volume-title":"Circulation","author":"Benjamin","year":"2019"},{"key":"2024103012043984500_CIT0002","first-page":"1240","article-title":"Trends of r-tPA (recombinant tissue-type plasminogen activator) treatment and treatment-influencing factors in acute ischemic 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