{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:08:59Z","timestamp":1790201339319,"version":"4.1.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100005297","name":"Medical University of Bialystok","doi-asserted-by":"publisher","award":["SUB\/1\/DN\/22\/001\/1166"],"award-info":[{"award-number":["SUB\/1\/DN\/22\/001\/1166"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Preclinical studies have demonstrated that the edible arboreal medicinal mushroom Hericium erinaceus (HE) mitigates high corticosterone levels and enhances stress-coping strategies in rodents, with emerging evidence suggesting similar neuroprotective potential for Fomitopsis officinalis (FO) and Pleurotus djamor (PDJ). However, comparative dose-dependent effects, including comprehensive anxiety and depression assessments, remain underexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>This study investigates the dose-dependent effects of dietary supplementation with HE, FO, or PDJ on anxiety-like behaviors and stress-coping strategies in rats, building on prior findings of their anxiolytic and antidepressant-like properties, and compares these effects to fluoxetine (FLX; 20\u00a0mg\/kg), a standard treatment for stress-related psychiatric disorders.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Male Wistar rats received 0, 100, 250, or 500\u00a0mg\/kg (per os) of HE, FO, or PDJ lyophilizate from in vitro cultures for 3\u00a0weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Behavioral outcomes were assessed using the open field test (OFT), elevated plus maze (EPM), and forced swim test (FST). Pearson\u2019s correlations explored relationships between FST parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A middle dose of FO (250\u00a0mg\/kg) increased rearing in the OFT, indicating reduced anxiety-like behavior. In the EPM, FO (100\u2013250\u00a0mg\/kg) and PDJ (100\u00a0mg\/kg) increased open arm entries and time while reducing closed arm time, indicating anxiolytic-like effects consistent with prior studies on HE and extending novel insights to PDJ. In the FST, FO (250\u00a0mg\/kg) reduced immobility, increased swimming, and decreased climbing, suggesting serotonergic-mediated antidepressant-like effects comparable or superior to FLX. HE and PDJ (250\u2013500\u00a0mg\/kg) reduced immobility and increased climbing or swimming, indicating dose-specific antidepressant effects. Immobility was strongly negatively correlated with swimming (r\u2009=\u2009\u22120.75, P\u2009&amp;lt;\u2009.0001) and moderately with climbing (r\u2009=\u2009\u22120.43, P\u2009&amp;lt;\u2009.001), with no significant correlation between swimming and climbing (r\u2009=\u2009\u22120.18, P =\u00a0.06).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study advances prior research by demonstrating that dietary supplementation with HE, FO, and PDJ exerts dose-dependent anxiolytic and antidepressant-like effects in rats, likely via serotonergic, noradrenergic, GABAergic, and anti-inflammatory pathways. The FST correlations highlight distinct coping mechanisms, supporting their potential as dietary interventions for stress-related disorders, comparable to FLX, warranting further investigation into underlying mechanisms and clinical applicability.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jpp\/rgaf082","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T11:27:44Z","timestamp":1756726064000},"page":"1787-1796","source":"Crossref","is-referenced-by-count":1,"title":["Effects of dietary supplementation with arboreal medicinal mushrooms on stress coping strategies, depressive and anxiety-like behavior of rats"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5510-8620","authenticated-orcid":false,"given":"Trofimiuk","family":"Emil","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology, Medical University of Bialystok ; Waszyngtona 15A, 15-274 Bialystok ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fija\u0142kowska","family":"Agata","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College , Medyczna 9, PL 30-688 Krak\u00f3w ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oniszczuk","family":"Hubert","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Medical University of Bialystok ; Waszyngtona 15A, 15-274 Bialystok ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Car","family":"Halina","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Medical University of Bialystok ; Waszyngtona 15A, 15-274 Bialystok ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muszy\u0144ska","family":"Bo\u017cena","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College , Medyczna 9, PL 30-688 Krak\u00f3w ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,9,17]]},"reference":[{"key":"2025120200421667700_ref1","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1523\/JNEUROSCI.0733-19.2019","article-title":"Revisiting the stress concept: implications for affective disorders","volume":"40","author":"McEwen","year":"2020","journal-title":"J Neurosci"},{"key":"2025120200421667700_ref2","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1016\/j.euroneuro.2019.10.011","article-title":"Nutritional psychiatry: towards improving mental health by what you eat","volume":"29","author":"Adan","year":"2019","journal-title":"Eur Neuropsychopharmacol"},{"key":"2025120200421667700_ref3","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1017\/S0029665117002026","article-title":"Nutritional psychiatry: the present state of the evidence","volume":"76","author":"Marx","year":"2017","journal-title":"Proc Nutr Soc"},{"key":"2025120200421667700_ref4","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1038\/s41380-018-0237-8","article-title":"Healthy dietary indices and risk of depressive outcomes: a systematic review and meta-analysis of observational studies","volume":"24","author":"Lassale","year":"2019","journal-title":"Mol Psychiatry"},{"key":"2025120200421667700_ref5","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.jad.2017.09.022","article-title":"Diet quality and depression risk: a systematic review and dose-response meta-analysis of prospective studies","volume":"226","author":"Molendijk","year":"2018","journal-title":"J Affect Disord"},{"key":"2025120200421667700_ref6","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1097\/PSY.0000000000000673","article-title":"The effects of dietary improvement on symptoms of depression and anxiety: a meta-analysis of randomized controlled trials","volume":"81","author":"Firth","year":"2019","journal-title":"Psychosom Med"},{"key":"2025120200421667700_ref7","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1186\/s12906-020-03132-x","article-title":"Neuroprotective effects of Hericium erinaceus (bull.: Fr.) 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