{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:27:46Z","timestamp":1790767666077,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Heilongjiang Touyan Innovation Team Program","award":["2019539"],"award-info":[{"award-number":["2019539"]}]},{"name":"Discipline Collaborative Innovation Achievement Project of Heilongjiang Province","award":["LJGXCG2023017"],"award-info":[{"award-number":["LJGXCG2023017"]}]},{"name":"Young Qihuang Scholar Project of Heilongjiang Province","award":["202336"],"award-info":[{"award-number":["202336"]}]},{"name":"National Key Research and Development Project of China","award":["2019YFC1708801"],"award-info":[{"award-number":["2019YFC1708801"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the mechanism and pharmacological activity of (Xiaoyaosan decoction) XYSD in the treatment of NAFL (nonalcoholic fatty liver) by proteomic techniques.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>C57BL\/6 mice NAFL model was induced by HFD (high fat diet), and observed biochemical indicators and histopathological changes. We employed TMT (tandem mass tag) quantitative proteomics and conducted bioinformatics analysis. Additionally, an in vitro model using unsaturated FFA (free fatty acid)-induced lipid accumulation was established to discover the effect of XYSD on lipid accumulation in HepG2 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results showed that XYSD improved liver index and biochemical indexes, and reversed the characteristics of liver steatosis in NAFL mice. Based on the results of in vivo proteomic enrichment analysis, we hypothesize that the activation of the PPAR\u03b1 signaling pathway may be a potential mechanism underlying the therapeutic effect of XYSD in treating NAFL. XYSD remarkably improved protein and gene expression of Fads2 (fatty acid desaturase 2), Hmgcs2 (3-hydroxymethylglutaryl-CoA synthase 2), Fabp1 (fatty acid-binding protein 1) and PPAR\u03b1. This hypothesis has been further confirmed in our in vitro experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study shows that XYSD improves lipid accumulation by activating PPAR\u03b1 signaling pathway, and finally achieves the effect of NAFL.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jpp\/rgaf109","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T11:15:43Z","timestamp":1763982943000},"source":"Crossref","is-referenced-by-count":1,"title":["Revealing action mechanisms of Xiaoyaosan decoction for the treatment of nonalcoholic fatty liver in mice employing tandem mass tag quantitative proteomics"],"prefix":"10.1093","volume":"78","author":[{"given":"Hong-Xiang","family":"Jiang","sequence":"first","affiliation":[{"name":"Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of 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,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education , 24 Heping Road, Harbin, Heilongjiang 150040 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Fei","family":"Wang","sequence":"additional","affiliation":[{"name":"Enzyme Engineering and Green Biomanufacturing Laboratory, College of Life Science, Northeast Agricultural University , 59 Wood Street, Xiangfang District, Harbin, Heilongjiang 150030 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0848-5658","authenticated-orcid":false,"given":"Jun","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese 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