{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:04:32Z","timestamp":1777500272792,"version":"3.51.4"},"reference-count":93,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Coordination of Superior Level Staff Improvement","award":["001"],"award-info":[{"award-number":["001"]}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["310467\/2023-3"],"award-info":[{"award-number":["310467\/2023-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study evaluated the analgesic efficacy of intranasally administered donepezil-loaded nanostructured lipid carriers (DPZ-NLCs) for neuropathic pain (NP), focusing on the involvement of cholinergic receptors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Male C57BL\/6 mice subjected to chronic sciatic nerve constriction were assessed for mechanical withdrawal threshold using the von Frey test. To identify receptor-specific mechanisms, selective antagonists of M2 muscarinic (M2 mAChRs) and \u03b17 nicotinic (\u03b17 nAChRs) receptors were administered before DPZ-NLCs. mRNA expression of these receptors, microglial markers, and cytokine levels were quantified in the spinal cord and thalamus by RT-PCR and ELISA. DPZ-NLCs effects on LPS-activated microglia were evaluated by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key findings<\/jats:title>\n                    <jats:p>Pharmacological data revealed that M2 mAChRs and \u03b17 nAChRs mediate the antinociceptive effects of DPZ-NLCs. Treatment increased \u03b17 nAChR mRNA expression in the thalamus, and this effect was blocked by intra-thalamic \u03b17 nAChR antagonism. DPZ-NLCs also reduced TNF-\u03b1 levels in the thalamus and in LPS-stimulated microglial cultures, indicating inhibition of microglial activation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Intranasal DPZ-NLCs show promise as a therapeutic strategy for NP, likely involving central M2 mAChR- and \u03b17 nAChR-dependent mechanisms. Limitations include the use of male mice only and the absence of pharmacokinetic assessments.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jpp\/rgag003","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T12:24:35Z","timestamp":1767702275000},"source":"Crossref","is-referenced-by-count":1,"title":["Nanostructured lipid carrier-mediated delivery of donepezil attenuates neuropathic pain via central cholinergic receptor modulation"],"prefix":"10.1093","volume":"78","author":[{"given":"Fernanda Santos","family":"Rocha","sequence":"first","affiliation":[{"name":"Laboratory of Neuroimmunobiology of Pain, Center for Experimental Biology, Federal University of Alfenas , 37131-001 Alfenas, Minas Gerais ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rayner Ribeiro","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Laboratory of Neuroimmunobiology of Pain, Center for Experimental Biology, 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