{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T16:54:21Z","timestamp":1774284861530,"version":"3.50.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The present study sought to elucidate the gastroprotective mechanism of the Akebiae Fructus\u2013 Hoveniae Semen combination by integrating chemical fingerprinting, network pharmacology and in vivo validation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>High-performance liquid chromatography (HPLC) fingerprints were established for 13 pairs of drug combinations. Core targets and pathways were predicted using network pharmacology and subsequently validated. in a mouse model of ethanol-induced acute gastric mucosal injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key findings<\/jats:title>\n                    <jats:p>HPLC fingerprinting revealed eight characteristic components. Network pharmacology analysis identified 29 hub targets (e.g. interleukin-6 (IL-6), matrix metalloproteinase-9 (MMP9) and B-cell lymphoma 2 (BCL2)) implicated in the interleukin-17 (IL-17), mitogen-activated protein kinase (MAPK) and tumor necrosis factor (TNF) signalling pathways. In vivo, the treatment combination demonstrated a dose-dependent reduction in gastric damage. It significantly decreased the elevated levels of interleukin-6 (IL-6), interleukin-1\u03b2 (IL-1\u03b2), tumor necrosis factor-\u03b1 (TNF-\u03b1), nitric oxide (NO), and malondialdehyde (MDA), while restoring interleukin-10 (IL-10) levels and superoxide dismutase (SOD) activity. The high-dose treatment exerted the most potent effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The combination exerts a potent protective effect against ethanol-induced gastric mucosal injury in mice, likely mediated through the modulation of inflammatory cytokines, oxidative stress markers and key protein targets in a dose-dependent manner. These findings provide a basis for its traditional use in gastrointestinal disorders.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jpp\/rgag023","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T15:57:58Z","timestamp":1774281478000},"source":"Crossref","is-referenced-by-count":0,"title":["From plant pair to gastroprotection: chemical standardization and in vivo validation of Akebiae Fructus-Hoveniae semen against ethanol-mediated mucosal damage"],"prefix":"10.1093","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3934-9195","authenticated-orcid":false,"given":"Yali","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Anhui University of Chinese Medicine , No. 350 Longzihu Road, Yaohai District, Hefei 230012, Anhui Province ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuqin","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Anhui University of Chinese Medicine , No. 350 Longzihu Road, Yaohai District, Hefei 230012, Anhui Province ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Anhui University of Chinese Medicine , No. 350 Longzihu Road, Yaohai District, Hefei 230012, Anhui Province ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyun","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Anhui University of Chinese Medicine , No. 350 Longzihu Road, Yaohai District, Hefei 230012, Anhui Province ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Anhui University of Chinese Medicine , No. 350 Longzihu Road, Yaohai District, Hefei 230012, Anhui Province ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinmei","family":"Ou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Anhui University of Chinese Medicine , No. 350 Longzihu Road, Yaohai District, Hefei 230012, Anhui Province ,","place":["China"]},{"name":"Anhui Key Laboratory of New Manufacturing Technology of Chinese Medicine Pieces, Anhui University of Chinese Medicine , No. 350 Longzihu Road, Yaohai District, Hefei 230012, Anhui Province ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"key":"2026032311575068600_ref1","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.ijbiomac.2017.08.175","article-title":"Protective effects of polysaccharide from Dendrobium nobile against ethanol-induced gastric damage in rats","volume":"107","author":"Zhang","year":"2018","journal-title":"Int J Biol Macromol"},{"key":"2026032311575068600_ref2","doi-asserted-by":"publisher","first-page":"112473","DOI":"10.1016\/j.jep.2019.112473","article-title":"Gastroprotective activity of hydroalcoholic extract of the leaves of Urera baccifera in rodents","volume":"250","author":"Benvenutti","year":"2020","journal-title":"J Ethnopharmacol"},{"key":"2026032311575068600_ref3","doi-asserted-by":"publisher","first-page":"4866","DOI":"10.3390\/nu15234866","article-title":"The Gastroprotective effect of walnut peptides: mechanisms and impact on ethanol-induced acute gastric mucosal injury in mice","volume":"15","author":"Yuan","year":"2023","journal-title":"Nutrients."},{"key":"2026032311575068600_ref4","doi-asserted-by":"publisher","first-page":"1989","DOI":"10.19540\/j.cnki.cjcmm.20220105.401","article-title":"Compatibility for toxicity attenuation of toxic traditional Chinese medicine: a review and strategies","volume":"47","author":"Gao","year":"2022","journal-title":"Zhongguo Zhong Yao Za Zhi"},{"key":"2026032311575068600_ref5","first-page":"433","article-title":"Clinical effect of the extract of TCM Fructus akebiae combined with ursodeoxycholic acid on nonalcoholic fatty liver disease","volume":"32","author":"Hongguang","year":"2019","journal-title":"Pak J Pharm Sci"},{"key":"2026032311575068600_ref6","doi-asserted-by":"publisher","first-page":"1337633","DOI":"10.3389\/fphar.2024.1337633","article-title":"Hovenia dulcis: a Chinese medicine that plays an essential role in alcohol-associated liver disease","volume":"15","author":"He","year":"2024","journal-title":"Front Pharmacol"},{"key":"2026032311575068600_ref7","doi-asserted-by":"publisher","DOI":"10.3390\/nu9010041"},{"key":"2026032311575068600_ref8","doi-asserted-by":"publisher","first-page":"116042","DOI":"10.1016\/j.jep.2022.116042","article-title":"Protective effect of Hovenia dulcis Thunb. 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