{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T14:50:03Z","timestamp":1776178203605,"version":"3.50.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Cerebral ischemia\u2013reperfusion injury (CIRI) presents a major therapeutic challenge in stroke management, where oxidative stress and neuroinflammation synergistically exacerbate neuronal damage. This study examined whether exogenous hydrogen sulphide (H\u2082S) protects against CIRI by simultaneously modulating the nuclear erythroid 2-related factor 2 (Nrf2)\/heme oxygenase 1 (HO-1) antioxidant response and inhibiting nuclear factor kappa-B (NF-\u03baB) and Nod-like receptor (NLR) family, pyrin domain-containing 3 (NLRP3) inflammasome engagement in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Animal models were subjected to middle cerebral artery occlusion and reperfusion (MCAO\/R) in mice, and neurological deficits were subsequently assessed using a rating scale and 2,3,5-triphenyltetrazolium chloride (TTC) staining to evaluate MCAO\/R damage. The expression level of reactive oxygen species (ROS) was detected using an assay kit. The levels of Nrf2\/HO-1 pathway and NLRP3 inflammasome were examined by Western blot and immunohistochemical staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key findings<\/jats:title>\n                    <jats:p>The experimental data showed that exogenous administration of H\u2082S was effective in improving behavioral disorders and reducing infarct volume. Treatment with exogenous H\u2082S regulated the expression of the Nrf2\/HO-1 signalling pathway, while H\u2082S effectively inhibited the expression of NF-\u03baB and the formation of the NLRP3 inflammasome, as evidenced by reduced levels of phosphorylated NF-\u03baB subunit p65 (p-p65), NLRP3, apoptosis-associated speck-like protein (ASC), and activated Caspase-1, and led to decreased maturation of the proinflammatory cytokines interleukin (IL)-1\u03b2 and IL-18.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study demonstrated that exogenous administration of H\u2082S exerted neuroprotective effects by regulating the Nrf2\/HO-1 antioxidant pathway and inhibiting NLRP3 inflammasome activation.<\/jats:p>\n                  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