{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T17:55:39Z","timestamp":1781546139600,"version":"3.54.5"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22H02781"],"award-info":[{"award-number":["JP22H02781"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K09956"],"award-info":[{"award-number":["JP24K09956"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008731","name":"Nakatomi Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Intrathecal administration of methotrexate (MTX) is used to treat cancer in the central nervous system; however, it sometimes causes neurotoxicity. Despite their clinical importance, the pharmacokinetics and the mechanism underlying the neurotoxicity of intrathecally administered MTX are largely unknown. This study aimed to clarify the involvement of breast cancer resistance protein (BCRP) in the pharmacokinetics and neurotoxicity of intrathecally administered MTX.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Plasma, brain, and cerebrospinal fluid (CSF) concentrations of MTX were compared between wild-type (WT) and Bcrp gene knockout mice. Choroid plexus (CP)-specific BCRP knockdown mice were constructed using adeno-associated virus encoding short hairpin RNA, and pharmacokinetic analysis and uptake studies in isolated CP were performed. Hippocampal neurogenesis was also evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key findings<\/jats:title>\n                    <jats:p>Plasma and brain concentrations of intrathecally, but not intravenously administered MTX, were significantly higher in Bcrp gene knockout mice than in WT mice. Plasma MTX concentration was higher in CP-specific BCRP knockdown mice than in the control. BCRP inhibition enhanced MTX accumulation in isolated CP. MTX-induced decline in hippocampal cellular proliferation was exacerbated in Bcrp gene knockout mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>BCRP may play an important role in MTX pharmacokinetics after intrathecal, but not intravenous, administration by regulating the CSF-to-blood transition in the CP and protecting against MTX-induced neurotoxicity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jpp\/rgag062","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T11:50:12Z","timestamp":1779450612000},"source":"Crossref","is-referenced-by-count":0,"title":["Role of breast cancer resistance protein in pharmacokinetics of intrathecally administered methotrexate in mice"],"prefix":"10.1093","volume":"78","author":[{"given":"Keiko","family":"Shintani","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192 ,","place":["Japan"]},{"name":"Department of Pharmacy, Kanazawa Municipal Hospital , Kanazawa 921-8105 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyet Kim","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Ishimoto","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayuka","family":"Itaya","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuya","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiya","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Masuo","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8135-5679","authenticated-orcid":false,"given":"Yukio","family":"Kato","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"2026061513274212400_ref1","doi-asserted-by":"publisher","first-page":"1094","DOI":"10.1056\/NEJM198311033091805","article-title":"The pharmacology and clinical use of methotrexate","volume":"309","author":"Jolivet","year":"1983","journal-title":"N Engl J Med"},{"key":"2026061513274212400_ref2","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1200\/JCO.2013.53.0808","article-title":"Methotrexate-induced neurotoxicity and leukoencephalopathy in childhood acute lymphoblastic leukemia","volume":"32","author":"Bhojwani","year":"2014","journal-title":"J Clin Oncol"},{"key":"2026061513274212400_ref3","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1097\/00043426-198921000-00019","article-title":"Neurotoxicity due to central nervous system therapy for childhood leukemia","volume":"11","author":"Ochs","year":"1989","journal-title":"J Pediatr Hematol Oncol"},{"key":"2026061513274212400_ref4","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1177\/08830738050200020901","article-title":"Cognitive changes in children treated for acute lymphoblastic leukemia with chemotherapy only according to the Pediatric Oncology Group 9605 protocol","volume":"20","author":"Montour-Proulx","year":"2005","journal-title":"J Child Neurol"},{"key":"2026061513274212400_ref5","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.neuropharm.2018.07.007","article-title":"Methotrexate causes persistent deficits in memory and executive function in a juvenile animal model","volume":"139","author":"Wen","year":"2018","journal-title":"Neuropharmacology"},{"key":"2026061513274212400_ref6","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13112837","article-title":"Systematic review of pharmacogenetic factors that influence high-dose methotrexate pharmacokinetics in pediatric malignancies","volume":"13","author":"Taylor","year":"2021","journal-title":"Cancers (Basel)"},{"key":"2026061513274212400_ref7","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.mam.2016.09.002","article-title":"The proton-coupled folate transporter (PCFT-SLC46A1) and the syndrome of systemic and cerebral folate deficiency of infancy: hereditary folate malabsorption","volume":"53","author":"Zhao","year":"2017","journal-title":"Mol Asp Med"},{"key":"2026061513274212400_ref8","doi-asserted-by":"publisher","first-page":"2059","DOI":"10.1097\/00001756-200211150-00014","article-title":"Localisation of breast cancer resistance protein in microvessel endothelium of human brain","volume":"13","author":"Cooray","year":"2002","journal-title":"Neuroreport"},{"key":"2026061513274212400_ref9","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/0006-8993(95)00511-n","article-title":"Isolation and culture of human brain microvessel endothelial cells for the study of blood-brain barrier properties in vitro","volume":"692","author":"Biegel","year":"1995","journal-title":"Brain Res"},{"key":"2026061513274212400_ref10","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/J.NEUROSCIENCE.2004.07.051","article-title":"Expression and immunolocalization of the multidrug resistance proteins, MRP1-MRP6 (ABCC1-ABCC6), in human brain","volume":"129","author":"Nies","year":"2004","journal-title":"Neuroscience"},{"key":"2026061513274212400_ref11","doi-asserted-by":"publisher","first-page":"3900","DOI":"10.1073\/pnas.96.7.3900","article-title":"Choroid plexus epithelial expression of MDR1 P glycoprotein and multidrug resistance-associated protein contribute to the blood-cerebrospinal-fluid drug-permeability barrier","volume":"96","author":"Rao","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026061513274212400_ref12","doi-asserted-by":"publisher","first-page":"2123","DOI":"10.1038\/ncomms3123","article-title":"Choroid plexus transcytosis and exosome shuttling deliver folate into brain parenchyma","volume":"4","author":"Grapp","year":"2013","journal-title":"Nat Commun"},{"key":"2026061513274212400_ref13","doi-asserted-by":"publisher","first-page":"7612","DOI":"10.1128\/mcb.24.17.7612-7621.2004","article-title":"Mrp4 confers resistance to topotecan and protects the brain from chemotherapy","volume":"24","author":"Leggas","year":"2004","journal-title":"Mol Cell Biol"},{"key":"2026061513274212400_ref14","doi-asserted-by":"publisher","first-page":"2667","DOI":"10.1007\/S11095-023-03598-7","article-title":"Functional evaluation of P-gp and Bcrp at the murine blood-cerebrospinal fluid barrier","volume":"40","author":"Sun","year":"2023","journal-title":"Pharm Res"},{"key":"2026061513274212400_ref15","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1111\/j.1471-4159.2005.03369.x","article-title":"Distinct spatio-temporal expression of ABCA and ABCG transporters in the developing and adult mouse brain","volume":"95","author":"Tachikawa","year":"2005","journal-title":"J Neurochem"},{"key":"2026061513274212400_ref16","doi-asserted-by":"publisher","first-page":"982","DOI":"10.1016\/S0026-895X(24)12193-1","article-title":"Expression and functional characterization of rat organic anion transporter 3 (rOat3) in the choroid plexus","volume":"61","author":"Nagata","year":"2002","journal-title":"Mol Pharmacol"},{"key":"2026061513274212400_ref17","doi-asserted-by":"publisher","first-page":"1638","DOI":"10.1248\/bpb.b16-00319","article-title":"Screening to identify multidrug resistance-associated protein inhibitors with neuroblastoma-selective cytotoxicity","volume":"39","author":"Nakamichi","year":"2016","journal-title":"Biol Pharm Bull"},{"key":"2026061513274212400_ref18","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1111\/j.1528-1167.2005.66604.x","article-title":"Localization of breast cancer resistance protein (BCRP) in microvessel endothelium of human control and epileptic brain","volume":"46","author":"Aronica","year":"2005","journal-title":"Epilepsia"},{"key":"2026061513274212400_ref19","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.neures.2005.01.013","article-title":"Functional expression of ABCG2 transporter in human neural stem\/progenitor cells","volume":"52","author":"Islam","year":"2005","journal-title":"Neurosci Res"},{"key":"2026061513274212400_ref20","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1124\/jpet.112.196543","article-title":"Inflammatory regulation of ATP binding cassette efflux transporter expression and function in microglia","volume":"343","author":"Gibson","year":"2012","journal-title":"J Pharmacol Exp Ther"},{"key":"2026061513274212400_ref21","doi-asserted-by":"publisher","first-page":"1003812","DOI":"10.3389\/fphar.2022.1003812","article-title":"Association between high-dose methotrexate-induced toxicity and polymorphisms within methotrexate pathway genes in acute lymphoblastic leukemia","volume":"13","author":"Xu","year":"2022","journal-title":"Front Pharmacol"},{"key":"2026061513274212400_ref22","doi-asserted-by":"publisher","first-page":"800","DOI":"10.22038\/IJBMS.2020.41754.9858","article-title":"Polymorphisms within methotrexate pathway genes: relationship between plasma methotrexate levels, toxicity experienced and outcome in pediatric acute lymphoblastic leukemia","volume":"23","author":"Esmaili","year":"2020","journal-title":"Iran J Basic Med Sci"},{"key":"2026061513274212400_ref23","doi-asserted-by":"publisher","first-page":"9388","DOI":"10.18632\/oncotarget.11543","article-title":"Pharmacogenetic analysis of high-dose methotrexate treatment in children with osteosarcoma","volume":"8","author":"Hegyi","year":"2017","journal-title":"Oncotarget"},{"key":"2026061513274212400_ref24","doi-asserted-by":"publisher","first-page":"20359","DOI":"10.1038\/s41598-019-56900-2","article-title":"Concomitant febuxostat enhances methotrexate-induced hepatotoxicity by inhibiting breast cancer resistance protein","volume":"9","author":"Ikemura","year":"2019","journal-title":"Sci Rep"},{"key":"2026061513274212400_ref25","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/s41576-019-0205-4","article-title":"Engineering adeno-associated virus vectors for gene therapy","volume":"21","author":"Li","year":"2020","journal-title":"Nat Rev Genet"},{"key":"2026061513274212400_ref26","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1089\/hum.2019.300","article-title":"Different serotypes of adeno-associated virus vector- and lentivirus-mediated tropism in choroid plexus by intracerebroventricular delivery","volume":"31","author":"Chen","year":"2020","journal-title":"Hum Gene Ther"},{"key":"2026061513274212400_ref27","doi-asserted-by":"publisher","first-page":"100426","DOI":"10.1016\/j.dmpk.2021.100426","article-title":"Species difference in brain penetration of P-gp and BCRP substrates among monkey, dog and mouse","volume":"42","author":"Kido","year":"2022","journal-title":"Drug Metab Pharmacokinet"},{"key":"2026061513274212400_ref28","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/0014-2999(80)90515-4","article-title":"Intrathecal morphine in mice: a new technique","volume":"67","author":"Hylden","year":"1980","journal-title":"Eur J Pharmacol"},{"key":"2026061513274212400_ref29","doi-asserted-by":"publisher","first-page":"1412","DOI":"10.1038\/nprot.2006.207","article-title":"Production and characterization of adeno-associated viral vectors","volume":"1","author":"Grieger","year":"2006","journal-title":"Nat Protoc"},{"key":"2026061513274212400_ref30","first-page":"162","article-title":"Method validation of methotrexate in human plasma by LC-MS technique in patients with brain tumour","volume":"3","author":"Danafar","year":"2016","journal-title":"Int J Epidemiol Res"},{"key":"2026061513274212400_ref31","doi-asserted-by":"publisher","first-page":"1479","DOI":"10.2116\/analsci.19P481","article-title":"Simultaneous determination of urine methotrexate, 7-hydroxy methotrexate, deoxyaminopteroic acid, and 7-hydroxy deoxyaminopteroic acid by UHPLC-MS\/MS in patients receiving high-dose methotrexate therapy","volume":"36","author":"Mei","year":"2020","journal-title":"Anal Sci"},{"key":"2026061513274212400_ref32","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1038\/s41538-024-00250-5","article-title":"TrkB phosphorylation in serum extracellular vesicles correlates with cognitive function enhanced by ergothioneine in humans","volume":"8","author":"Ishimoto","year":"2024","journal-title":"NPJ Sci Food"},{"key":"2026061513274212400_ref33","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1016\/j.tjnut.2024.12.021","article-title":"Trideoxycytidine diphosphate promotes neural stem cell proliferation and neurogenesis in mice","volume":"155","author":"Ishimoto","year":"2025","journal-title":"J Nutr"},{"key":"2026061513274212400_ref34","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/j.cellsig.2018.10.012","article-title":"Ergothioneine-induced neuronal differentiation is mediated through activation of S6K1 and neurotrophin 4\/5-TrkB signaling in murine neural stem cells","volume":"53","author":"Ishimoto","year":"2019","journal-title":"Cell Signal"},{"key":"2026061513274212400_ref35","doi-asserted-by":"publisher","first-page":"e89434","DOI":"10.1371\/journal.pone.0089434","article-title":"Organic cation transporter-mediated ergothioneine uptake in mouse neural progenitor cells suppresses proliferation and promotes differentiation into neurons","volume":"9","author":"Ishimoto","year":"2014","journal-title":"PLoS One"},{"key":"2026061513274212400_ref36","first-page":"703","article-title":"Clinical pharmacology of intrathecal methotrexate. I. Pharmacokinetics in nontoxic patients after lumbar injection","volume":"61","author":"Bleyer","year":"1977","journal-title":"Cancer Treat Rep"},{"key":"2026061513274212400_ref37","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1056\/NEJM197507242930402","article-title":"Methotrexate: distribution in cerebrospinal fluid after intravenous, ventricular and lumbar injections","volume":"293","author":"Shapiro","year":"1975","journal-title":"N Engl J Med"},{"key":"2026061513274212400_ref38","doi-asserted-by":"publisher","first-page":"e35613","DOI":"10.1371\/journal.pone.0035613","article-title":"ABC transporters B1, C1 and G2 differentially regulate neuroregeneration in mice","volume":"7","author":"Schumacher","year":"2012","journal-title":"PLoS One"},{"key":"2026061513274212400_ref39","first-page":"417","article-title":"Potent and specific inhibition of the breast cancer resistance protein multidrug transporter in vitro and in mouse intestine by a novel analogue of fumitremorgin C","volume":"1","author":"Allen","year":"2002","journal-title":"Mol Cancer Ther"},{"key":"2026061513274212400_ref40","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1124\/dmd.113.055228","article-title":"The effect of ABCG2 and ABCC4 on the pharmacokinetics of methotrexate in the brain","volume":"42","author":"Sane","year":"2014","journal-title":"Drug Metab Dispos"},{"key":"2026061513274212400_ref41","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.ejpb.2016.04.012","article-title":"PBPK model of methotrexate in cerebrospinal fluid ventricles using a combined microdialysis and MRI acquisition","volume":"104","author":"Brandhonneur","year":"2016","journal-title":"Eur J Pharm Biopharm"},{"key":"2026061513274212400_ref42","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1111\/j.1471-4159.2011.07208.x","article-title":"Quantitative targeted absolute proteomics of human blood-brain barrier transporters and receptors","volume":"117","author":"Uchida","year":"2011","journal-title":"J Neurochem"},{"key":"2026061513274212400_ref43","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1111\/jnc.13147","article-title":"Quantitative targeted absolute proteomics of rat blood-cerebrospinal fluid barrier transporters: comparison with a human specimen","volume":"134","author":"Uchida","year":"2015","journal-title":"J Neurochem"},{"key":"2026061513274212400_ref44","doi-asserted-by":"publisher","first-page":"936","DOI":"10.3390\/nu11040936","article-title":"Hesperidin alleviates methotrexate-induced memory deficits via hippocampal neurogenesis in adult rats","volume":"11","author":"Naewla","year":"2019","journal-title":"Nutrients"},{"key":"2026061513274212400_ref45","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.bbr.2009.02.025","article-title":"Methotrexate decreases hippocampal cell proliferation and induces memory deficits in rats","volume":"201","author":"Seigers","year":"2009","journal-title":"Behav Brain Res"},{"key":"2026061513274212400_ref46","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110651","article-title":"Metformin alleviates memory and hippocampal neurogenesis decline induced by methotrexate chemotherapy in a rat model","volume":"131","author":"Sritawan","year":"2020","journal-title":"Biomed Pharmacother"},{"key":"2026061513274212400_ref47","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.bbr.2007.08.004","article-title":"Long-lasting suppression of hippocampal cell proliferation and impaired cognitive performance by methotrexate in the rat","volume":"186","author":"Seigers","year":"2008","journal-title":"Behav Brain Res"},{"key":"2026061513274212400_ref48","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.bcp.2011.03.020","article-title":"Neurotoxicity of methotrexate to hippocampal cells in vivo and in vitro","volume":"82","author":"Yang","year":"2011","journal-title":"Biochem Pharmacol"},{"key":"2026061513274212400_ref49","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1080\/10428190802603169","article-title":"Little dose, huge toxicity: profound hematological toxicity of intrathecal methotrexate","volume":"50","author":"Kose","year":"2009","journal-title":"Leuk Lymphoma"},{"key":"2026061513274212400_ref50","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1248\/yakushi.17-00122","article-title":"A survey of the adverse effects and influence of concomitant drugs for methotrexate intrathecal administration","volume":"138","author":"Uchiyama","year":"2018","journal-title":"Yakugaku Zasshi"},{"key":"2026061513274212400_ref51","doi-asserted-by":"publisher","first-page":"bqae081","DOI":"10.1210\/endocr\/bqae081","article-title":"Nongenomic ER\u03b1-AMPK signaling regulates sex-dependent Bcrp transport activity at the blood-brain barrier","volume":"165","author":"Banks","year":"2024","journal-title":"Endocrinology"},{"key":"2026061513274212400_ref52","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1124\/mol.106.028167","article-title":"The effect of low pH on breast cancer resistance protein (ABCG2)-mediated transport of methotrexate, 7-hydroxymethotrexate, methotrexate diglutamate, folic acid, mitoxantrone, topotecan, and resveratrol in in vitro drug transport models","volume":"71","author":"Breedveld","year":"2007","journal-title":"Mol Pharmacol"},{"key":"2026061513274212400_ref53","first-page":"5538","article-title":"Wild-type breast cancer resistance protein (BCRP\/ABCG2) is a methotrexate polyglutamate transporter","volume":"63","author":"Volk","year":"2003","journal-title":"Cancer Res"},{"key":"2026061513274212400_ref54","first-page":"4048","article-title":"Transport of methotrexate, methotrexate polyglutamates, and 17beta-estradiol 17-(beta-D-glucuronide) by ABCG2: effects of acquired mutations at R482 on methotrexate transport","volume":"63","author":"Chen","year":"2003","journal-title":"Cancer Res"}],"container-title":["Journal of Pharmacy and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/78\/6\/rgag062\/68529566\/rgag062.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/78\/6\/rgag062\/68529566\/rgag062.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T17:27:51Z","timestamp":1781544471000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jpp\/article\/doi\/10.1093\/jpp\/rgag062\/8708280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":54,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6,2]]}},"URL":"https:\/\/doi.org\/10.1093\/jpp\/rgag062","relation":{},"ISSN":["2042-7158"],"issn-type":[{"value":"2042-7158","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,6]]},"published":{"date-parts":[[2026,6]]},"article-number":"rgag062"}}