{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:24:16Z","timestamp":1787682256403,"version":"build-2784847793"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC3501700"],"award-info":[{"award-number":["2022YFC3501700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274059"],"award-info":[{"award-number":["82274059"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["22S21901900"],"award-info":[{"award-number":["22S21901900"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Organizational Key Research and Development Program of Shanghai University of Traditional Chinese Medicine","award":["2023YZZ02"],"award-info":[{"award-number":["2023YZZ02"]}]},{"name":"Naval Military Medical University, Far East Talent Project","award":["SL33"],"award-info":[{"award-number":["SL33"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Ischemic stroke is a worldwide problem associated with substantial morbidity, disability and mortality. Yiqi Xingnao oral liquid (YQXN), derived from Buyanghuanwu Decoction, is clinically effective but mechanistically unclear. This study aimed to identify blood-absorbed components of YQXN and elucidate its therapeutic material basis and potential molecular mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Middle cerebral artery occlusion rats modelled ischemic stroke, and chemical ingredients of YQXN were analysed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Network pharmacology, molecular docking analyses, enzyme linked immunosorbent assay (ELISA), and western blot were performed to identify potential targets and investigate the mechanisms of action of YQXN. Moreover, cellular thermal shift assay (CETSA) experiment was performed to evaluate the effect of formononetin on the thermal stability of STAT3 and PTGS2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key findings<\/jats:title>\n                    <jats:p>A total of 141 chemical compounds were identified, with 23 detected in systemic circulation. Key targets including STAT3, PTGS2, tumour necrosis factor-\u03b1, interleukin-6, and interleukin-1\u03b2 were selected. Enrichment analyses indicated that YQXN may alleviate inflammatory responses by modulating the neuroactive ligand-receptor interaction pathway. Nine key active components (including calycosin, ononin, formononetin, cryptotanshinone, salvianolic acid G, senkyunolide A, ferulic acid, kaempferol, and caffeic acid) showed significant binding affinity for both PTGS2 and STAT3. Western blot and ELISA results confirmed that YQXN modulated the protein levels of these targets. Furthermore, a cell lysate-based CETSA showed that formononetin enhanced the thermal stability of STAT3 and PTGS2, supporting its potential target engagement with these proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>YQXN constituents target STAT3, PTGS2, and inflammatory cytokines to ameliorate ischemic stroke, providing a theoretical basis for its clinical application.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jpp\/rgag091","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:00:16Z","timestamp":1785412816000},"source":"Crossref","is-referenced-by-count":0,"title":["Chemical ingredients and molecular mechanisms underlying Yiqi Xingnao oral liquid in alleviating cerebral ischemic stroke based on UHPLC-Q-TOF-MS\/MS and integrated network 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Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine , Shanghai, 201203 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Changzheng Hospital, Naval Medical University (Second Military Medical University) , Shanghai, 200003 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Changzheng Hospital, Naval Medical University (Second Military Medical University) , Shanghai, 200003 ,","place":["China"]},{"name":"School of Pharmacy, Anhui University of Chinese Medicine , Hefei Anhui 230012 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leran","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine , Shanghai, 201203 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Xiu","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Changzheng Hospital, Naval Medical University (Second Military Medical University) , Shanghai, 200003 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Pang","sequence":"additional","affiliation":[{"name":"Tongji University School of Medicine Affiliated Shanghai Baoshan District Songnan Town Community Health Center , Shanghai, 200411 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Changzheng Hospital, Naval Medical University (Second Military Medical University) , Shanghai, 200003 ,","place":["China"]},{"name":"School of Pharmacy, Anhui University of Chinese Medicine , Hefei Anhui 230012 ,","place":["China"]},{"name":"School of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine , Shanghai, 201203 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"2026082513481232100_ref1","doi-asserted-by":"publisher","first-page":"946","DOI":"10.1177\/17474930221123175","article-title":"Global stroke statistics 2022","volume":"17","author":"Thayabaranathan","year":"2022","journal-title":"Int J Stroke"},{"key":"2026082513481232100_ref2","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.pcad.2025.11.003","article-title":"Lipid management for primary and secondary stroke prevention consensus paper of the International Lipid Expert Panel (ILEP)","volume":"94","author":"Banach","year":"2026","journal-title":"Prog Cardiovasc 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