{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T16:07:27Z","timestamp":1780762047250,"version":"3.54.1"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T00:00:00Z","timestamp":1697328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K07648"],"award-info":[{"award-number":["21K07648"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K07680"],"award-info":[{"award-number":["21K07680"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K07671"],"award-info":[{"award-number":["22K07671"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K07161"],"award-info":[{"award-number":["23K07161"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K14923"],"award-info":[{"award-number":["23K14923"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Radiation can induce DNA double-stranded breaks, which are typically detected by the fluorescence of phosphorylated histone H2AX. In this study, we examined the usefulness of the dynamics of radiation-induced gamma-H2AX foci of peripheral blood lymphocytes (PBLs), as a marker of DNA repair ability, in predicting late adverse events from radiotherapy. A total of 46 patients with cervical, vaginal and anal canal cancers treated with radical radiotherapy between 2014 and 2019 were included in this analysis. Concurrent chemotherapy was administered in 36 cases (78.3%). Peripheral blood was obtained before treatment, and then irradiated ex vivo with 1\u00a0Gy X-ray. The ratio of radiation-induced gamma-H2AX foci in PBLs measured at 30\u00a0min and at 4\u00a0h was defined as the foci decay ratio (FDR). With a median follow-up of 54\u00a0months, 9 patients (19.6%) were observed to have late genitourinary or gastrointestinal (GU\/GI) toxicity. The FDR ranged from 0.51 to 0.74 (median 0.59), with a significantly higher incidence of Grade 1 or higher late adverse events in the FDR\u2009\u2265\u20090.59 group. In multivariate analysis, FDR\u2009\u2265\u20090.59 and hypertension also emerged as significant factors associated with the development of late toxicities. Overall, our results suggest that measurement of radiation-induced gamma-H2AX foci in PBLs may predict the risk of late GU\/GI toxicities from chemoradiotherapy, which can enable tailoring the radiation dose to minimize adverse effects.<\/jats:p>","DOI":"10.1093\/jrr\/rrad079","type":"journal-article","created":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T21:54:47Z","timestamp":1697406887000},"page":"948-953","source":"Crossref","is-referenced-by-count":7,"title":["Prediction of late adverse events in pelvic cancer patients receiving definitive radiotherapy using radiation-induced gamma-H2AX foci assay"],"prefix":"10.1093","volume":"64","author":[{"given":"Masanori","family":"Someya","sequence":"first","affiliation":[{"name":"Department of Radiology , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"},{"name":"Sapporo Medical University , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , 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Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mio","family":"Kitagawa","sequence":"additional","affiliation":[{"name":"Department of Radiology , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"},{"name":"Sapporo Medical University , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Gocho","sequence":"additional","affiliation":[{"name":"Department of Radiology , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"},{"name":"Sapporo Medical University , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Mafune","sequence":"additional","affiliation":[{"name":"Department of Radiology , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"},{"name":"Sapporo Medical University , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaro","family":"Ikeuchi","sequence":"additional","affiliation":[{"name":"Department of Radiology , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"},{"name":"Sapporo Medical University , School of Medicine, , S1W16, Chuo-ku, Sapporo, Hokkaido 060-8543 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Tauchi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences , Faculty of Sciences, , Bunkyo 2-1-1, Mito, Ibaraki 310-8512 , Japan"},{"name":"Ibaraki University , Faculty of Sciences, , Bunkyo 2-1-1, Mito, Ibaraki 310-8512 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koh-ichi","family":"Sakata","sequence":"additional","affiliation":[{"name":"Department of Radiology , School of 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