{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:08:21Z","timestamp":1740136101544,"version":"3.37.3"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP23zf0127008"],"award-info":[{"award-number":["JP23zf0127008"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Fractionated total body irradiation (TBI) with X-rays induces thymic lymphoma\/leukemia (TL) in C57BL\/6 mice. Radiation-induced mouse TL (RITL) can be prevented by bone marrow transplantation (BMT) of unirradiated BM cells. However, the mechanisms underlying the prevention of RITL with BMT remain unclear. Here, we show that BMT restores thymic T-cell differentiation in mice subjected to TBI. TBI (four times of 1.8\u00a0Gy X-rays weekly) was conducted with C57BL\/6 mice. BMT was performed immediately after the last irradiation of TBI in mice by transplantation of BM cells isolated from enhanced green fluorescence protein (eGFP) transgenic mice. Thymic cell numbers were drastically decreased in TBI and TBI\u2009+\u2009BMT mice compared to those in non-irradiated mice. Flow cytometry showed a dramatic decrease in double negative (DN, CD4\u2212CD8\u2212) thymocytes, especially DN2 (CD25+CD44+) and DN3 (CD25+CD44\u2212) subpopulations, in the TBI mice on Day 10 after the last irradiation. In contrast, the DN2 and DN3 populations were recovered in TBI\u2009+\u2009BMT mice. Interestingly, these restored DN2 and DN3 cells mainly differentiated from eGFP-negative recipient cells but not from eGFP-positive donor cells, suggesting that transplanted BM cells may interact with recipient cells to restore thymic T-cell development in the RITL model. Taken together, our findings highlight the significance of restoring thymic T-cell differentiation by BMT in RITL prevention.<\/jats:p>","DOI":"10.1093\/jrr\/rrae045","type":"journal-article","created":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T07:53:50Z","timestamp":1718783630000},"page":"555-560","source":"Crossref","is-referenced-by-count":0,"title":["Restoration of thymic T-cell development by bone marrow transplantation in mouse radiation lymphomagenesis"],"prefix":"10.1093","volume":"65","author":[{"given":"Tsuguhide","family":"Takeshima","sequence":"first","affiliation":[{"name":"Department of Charged Particle Therapy Research, National Institutes for Quantum Science and Technology , Chiba 263-8555 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumitaka","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Charged Particle Therapy Research, National Institutes for Quantum Science and Technology , Chiba 263-8555 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"2024072301302789000_ref1","first-page":"185","article-title":"A quantitative dose-response study of lymphoid-tumor development in irradiated C 57 black mice","volume":"13","author":"Kaplan","year":"1952","journal-title":"J Natl Cancer Inst"},{"key":"2024072301302789000_ref2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1093\/mutage\/gew019","article-title":"Mouse models for radiation-induced cancers","volume":"31","author":"Rivina","year":"2016","journal-title":"Mutagenesis"},{"key":"2024072301302789000_ref3","doi-asserted-by":"crossref","first-page":"2026","DOI":"10.4049\/jimmunol.134.3.2026","article-title":"Cellular events during radiation-induced thymic leukemogenesis in mice: abnormal T cell differentiation in the thymus and defect of thymocyte precursors in the bone marrow after split-dose irradiation","volume":"134","author":"Muto","year":"1985","journal-title":"J Immunol"},{"key":"2024072301302789000_ref4","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1269\/jrr.32.SUPPLEMENT2_168","article-title":"Bone marrow-thymus interactions during thymic lymphomagenesis induced by fractionated radiation exposure in B10 mice: analysis using bone marrow transplantation between thy 1 congenic mice","volume":"32","author":"Sado","year":"1991","journal-title":"J Radiat Res"},{"key":"2024072301302789000_ref5","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1111\/j.1349-7006.2006.00218.x","article-title":"Radiation carcinogenesis in mouse thymic lymphomas","volume":"97","author":"Kominami","year":"2006","journal-title":"Cancer Sci"},{"key":"2024072301302789000_ref6","first-page":"677","article-title":"Influence of postirradiation thymectomy and of thymic implants on lymphoid tumor incidence in C57BL mice","volume":"13","author":"Kaplan","year":"1953","journal-title":"Cancer Res"},{"key":"2024072301302789000_ref7","first-page":"422","article-title":"Indirect induction of lymphomas in irradiated mice. 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