{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T10:06:46Z","timestamp":1779790006102,"version":"3.53.1"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Traditional Chinese Medicine Research Project of Wuhan Municipal Health Commission","award":["WZ24A25"],"award-info":[{"award-number":["WZ24A25"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Functional impairment of T and NK cells following irradiation (IR) undermine anti-tumor immune responses. This study aimed to investigate the characteristics of T-cell and NK-cell reconstitution in mice following low-dose radiation-induced injury and the restorative effects of Rehmanniae Radix Praeparata (RRP). At various time points post-IR, splenic\/thymic indices, complete blood count and CD3+ T, CD4+ T, CD8+ T and NK cells, as well as CD80, CD86, MHC-I and MHC-II were assessed. Type 1\u00a0T helper (Th1), Type 1 cytotoxic (Tc1), Type 1 NK (NK1), regulatory T (Treg) and Th17 cells along with IL-12, IL-15, T-bet and foxP3 were also evaluated with or without RRP treatment. Additionally, a B16 melanoma lung metastasis model in irradiated mice was used to confirm the restorative effects of RRP. CD3+, CD4+, CD8+ T and NK cells decreased significantly on Days 4 to 6 and largely recovered by Days 9 to 11 post-IR. The expression of CD80, CD86 and MHC-II reduced on Day 6 and recovered by Day 11. However, interferon (IFN)-\u03b3 production by Th1, Tc1 and NK1 cells remained lower, whereas Tregs were elevated. RRP effectively enhanced IFN-\u03b3 production from Th1, Tc1 and NK1 cells and suppressed Tregs by increasing T-bet and decreasing foxP3 expression, thereby significantly reduced the tumor burden. These findings suggest that T-Cell and NK-Cell-mediated inefficient reconstitution following IR was characterized by an decrease of Th1, Tc1 and NK1 cells and an increase of Tregs, RRP effectively promoted the functional reconstitution of T-cell and NK-cell subsets, thereby enhancing anti-tumor immunity after IR.<\/jats:p>","DOI":"10.1093\/jrr\/rrag020","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T12:32:42Z","timestamp":1772973162000},"page":"327-338","source":"Crossref","is-referenced-by-count":0,"title":["Restorative effect of Rehmanniae radix praeparata on T-cell and NK-cell-mediated immune reconstitution in mice following radiation-induced injury"],"prefix":"10.1093","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1494-3202","authenticated-orcid":false,"given":"Xiaodan","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Wuhan Hankou Hospital , 7 Erqi Side Road, Wuhan, Hubei 430010 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyun","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Neurology, People\u2019s Hospital of Dongxihu District , 48 Jinbei 1st Road, Wuhan, Hubei 430040 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhai","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Wuhan Hankou Hospital , 7 Erqi Side Road, Wuhan, Hubei 430010 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyun","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Wuhan Hankou Hospital , 7 Erqi Side Road, Wuhan, Hubei 430010 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hairui","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Wuhan Hankou Hospital , 7 Erqi Side 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