{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:09:36Z","timestamp":1784074176414,"version":"3.55.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,1,7]],"date-time":"2020-01-07T00:00:00Z","timestamp":1578355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25220102"],"award-info":[{"award-number":["25220102"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,3,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The risk of cancer due to exposure to ionizing radiation is higher in infants than in adults. In a previous study, the effect of adult-onset calorie restriction (CR) on carcinogenesis in mice after early-life exposure to X-rays was examined (Shang, Y, Kakinuma, S, Yamauchi, K, et al. Cancer prevention by adult-onset calorie restriction after infant exposure to ionizing radiation in B6C3F1 male mice. Int J Cancer. 2014; 135: 1038-47). The results showed that the tumor frequency was reduced in the CR group. However, the mechanism of tumor suppression by CR is not yet clear. In this study, we examined the effects of CR on radiation-induced mutations using gpt delta mice, which are useful to analyze mutations in various tissues throughout the whole body. Infant male mice (1-week old) were exposed to 3.8\u00a0Gy X-rays and fed a control (95\u00a0kcal\/week\/mouse) or CR (65\u00a0kcal\/week\/mouse) diet from adult stage (7-weeks old). Mice were sacrificed at the age of 7\u00a0weeks, 8\u00a0weeks and 100\u00a0days, and organs (spleen, liver, lung, thymus) were harvested. Mutations at the gpt gene in the DNA from the spleen were analyzed by using a gpt assay protocol that detects primarily point mutations in the gpt gene. The results showed that mutation frequencies were decreased in CR groups compared with non-CR groups. Sequence analysis of the gpt gene in mutants revealed a reduction in the G:C to T:A transversion in CR groups. Since it is known that 8-oxoguanine could result in this base substitution and that CR has an effect of reducing oxidative stress, these results indicate that the suppression of oxidative stress by CR is the cause of the reduction of this transversion.<\/jats:p>","DOI":"10.1093\/jrr\/rrz078","type":"journal-article","created":{"date-parts":[[2019,10,26]],"date-time":"2019-10-26T11:09:49Z","timestamp":1572088189000},"page":"187-196","source":"Crossref","is-referenced-by-count":6,"title":["The effects of short-term calorie restriction on mutations in the spleen cells of infant-irradiated mice"],"prefix":"10.1093","volume":"61","author":[{"given":"Saori","family":"Kakomi","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takafumi","family":"Nakayama","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan"},{"name":"National Institute of Radiological Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Shang","sequence":"first","affiliation":[{"name":"National Institute of Radiological Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chizuru","family":"Tsuruoka","sequence":"first","affiliation":[{"name":"National Institute of Radiological Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Sunaoshi","sequence":"first","affiliation":[{"name":"National Institute of Radiological Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takamitsu","family":"Morioka","sequence":"first","affiliation":[{"name":"National Institute of Radiological Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiya","family":"Shimada","sequence":"first","affiliation":[{"name":"National Institute of Radiological Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizuko","family":"Kakinuma","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan"},{"name":"National Institute of Radiological Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Tachibana","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,7]]},"reference":[{"key":"2020052418595308800_ref1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00247-006-0191-5","article-title":"Cancer risks following diagnostic and therapeutic radiation exposure in children","volume":"36","author":"Kleinerman","year":"2006","journal-title":"Pediatr Radiol."},{"key":"2020052418595308800_ref2","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1667\/RR2629.1","article-title":"Studies of the 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intake","volume":"81","author":"Gross","year":"1984","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020052418595308800_ref6","doi-asserted-by":"crossref","first-page":"7928","DOI":"10.1073\/pnas.83.20.7928","article-title":"Inhibition of the development of radiation-induced leukemia in mice by reduction of food intake","volume":"83","author":"Gross","year":"1986","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020052418595308800_ref7","doi-asserted-by":"crossref","first-page":"6795","DOI":"10.1073\/pnas.87.17.6795","article-title":"Prevention of spontaneous and radiation-induced tumors in rats by reduction of food intake","volume":"87","author":"Gross","year":"1990","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020052418595308800_ref8","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1073\/pnas.94.6.2615","article-title":"Calorie restriction reduces the incidence of myeloid leukemia induced by a single whole-body radiation in C3H\/He mice","volume":"94","author":"Yoshida","year":"1997","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020052418595308800_ref9","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1002\/ijc.28751","article-title":"Cancer prevention by adult-onset calorie restriction after infant exposure to ionizing radiation in B6C3F1 male mice","volume":"135","author":"Shang","year":"2014","journal-title":"Int J Cancer."},{"key":"2020052418595308800_ref10","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1001\/jama.297.9.986","article-title":"Aging, adiposity, and calorie restriction","volume":"297","author":"Fontana","year":"2007","journal-title":"Jama."},{"key":"2020052418595308800_ref11","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev.med.54.101601.152156","article-title":"Calorie restriction, aging, and cancer prevention: Mechanisms of action and applicability to humans","volume":"54","author":"Hursting","year":"2003","journal-title":"Annu Rev Med."},{"key":"2020052418595308800_ref12","doi-asserted-by":"crossref","first-page":"B521","DOI":"10.1093\/gerona\/54.12.B521","article-title":"Pleiotropic effects of growth hormone and insulin-like growth factor (IGF)-1 on biological aging: Inferences from moderate caloric-restricted animals","volume":"54","author":"Sonntag","year":"1999","journal-title":"J Gerontol A Biol Sci Med Sci."},{"key":"2020052418595308800_ref13","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1126\/science.273.5271.59","article-title":"Oxidative stress, caloric restriction, and aging","volume":"273","author":"Sohal","year":"1996","journal-title":"Science."},{"key":"2020052418595308800_ref14","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1093\/jn\/131.8.2139","article-title":"Inflammatory responses to lipopolysaccharide are suppressed in 40% energy-restricted mice","volume":"131","author":"Matsuzaki","year":"2001","journal-title":"J Nutr."},{"key":"2020052418595308800_ref15","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/j.exger.2012.11.007","article-title":"Recent advances in calorie restriction research on aging","volume":"48","author":"Chung","year":"2013","journal-title":"Exp Gerontol."},{"key":"2020052418595308800_ref16","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.ceb.2008.02.005","article-title":"FoxO transcription factors in the maintenance of cellular homeostasis during aging","volume":"20","author":"Salih","year":"2008","journal-title":"Curr Opin Cell Biol."},{"key":"2020052418595308800_ref17","first-page":"1851","article-title":"Influence of diet and calorie restriction on the initiation and promotion of skin carcinogenesis in the SENCAR mouse model","volume":"51","author":"Birt","year":"1991","journal-title":"Cancer 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Mutagen."},{"key":"2020052418595308800_ref24","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1002\/(SICI)1098-2280(1999)34:1<9::AID-EM2>3.0.CO;2-E","article-title":"Spi(\u2212) selection: An efficient method to detect gamma-ray-induced deletions in transgenic mice","volume":"34","author":"Nohmi","year":"1999","journal-title":"Environ Mol Mutagen."},{"key":"2020052418595308800_ref25","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0027-5107(00)00077-4","article-title":"Recent advances in the protocols of transgenic mouse mutation assays","volume":"455","author":"Nohmi","year":"2000","journal-title":"Mutat Res."},{"key":"2020052418595308800_ref26","doi-asserted-by":"crossref","first-page":"3354","DOI":"10.1073\/pnas.84.10.3354","article-title":"Sequence analysis of spontaneous mutations in a shuttle vector gene integrated into mammalian chromosomal DNA","volume":"84","author":"Ashman","year":"1987","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020052418595308800_ref27","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0027-5107(88)90240-0","article-title":"Examination of vectors with two dominant, selectable genes for DNA repair and mutation studies in mammalian cells","volume":"199","author":"Debenham","year":"1988","journal-title":"Mutat Res."},{"key":"2020052418595308800_ref28","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1002\/em.22132","article-title":"Tissue-specific and time-dependent clonal expansion of ENU-induced mutant cells in gpt delta mice","volume":"58","author":"Nakayama","year":"2017","journal-title":"Environ Mol Mutagen."},{"key":"2020052418595308800_ref29","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.mrfmmm.2007.12.001","article-title":"Differential effects of low- and high-dose X-rays on N-ethyl-N-nitrosourea-induced mutagenesis in thymocytes of B6C3F1 gpt-delta mice","volume":"640","author":"Yamauchi","year":"2008","journal-title":"Mutat 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damage","volume":"21","author":"Marnett","year":"2000","journal-title":"Carcinogenesis."},{"key":"2020052418595308800_ref33","doi-asserted-by":"crossref","first-page":"7485","DOI":"10.1093\/nar\/gkm860","article-title":"Caloric restriction and genomic stability","volume":"35","author":"Heydari","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"2020052418595308800_ref34","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.canlet.2011.12.012","article-title":"Ionizing radiation-induced metabolic oxidative stress and prolonged cell injury","volume":"327","author":"Azzam","year":"2012","journal-title":"Cancer Lett."},{"key":"2020052418595308800_ref35","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1080\/09553000410001692726","article-title":"Chronic oxidative stress and radiation-induced late normal tissue injury: A review","volume":"80","author":"Robbins","year":"2004","journal-title":"Int J Radiat Biol."},{"key":"2020052418595308800_ref36","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0165-1110(91)90029-U","article-title":"Ionizing radiation and genetic risks III. 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