{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:14:32Z","timestamp":1787066072305,"version":"build-2736575974"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":279,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Central Virginia Center on Drug Abuse Research","award":["5P30DA033934"],"award-info":[{"award-number":["5P30DA033934"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1HL147562"],"award-info":[{"award-number":["RO1HL147562"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institutes of Health IGNITE KUH Training Core","award":["5TL1DK132771"],"award-info":[{"award-number":["5TL1DK132771"]}]},{"name":"National Institutes of Health Sickle Cell Disease Pain Analgesia And Integrative Network","award":["1U24AT012868-01"],"award-info":[{"award-number":["1U24AT012868-01"]}]},{"DOI":"10.13039\/100017068","name":"Southern Regional Education Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017068","id-type":"DOI","asserted-by":"publisher"}]},{"name":"VCU Breakthrough"},{"name":"VCU School of Pharmacy Start Up funds"},{"name":"VCU School of Medicine Bridge Funds"},{"name":"Children\u2019s Health Research Institute Fellows Research Program at the Children\u2019s Hospital of Richmond"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>SCD is associated with morbidity, mortality, and severe pain that is well modeled in humanized Berkeley SCD (HbSS) mice. Here, we conducted a comprehensive study to evaluate the effects of age on the development of the HbSS hyper-nociceptive phenotype. We also examined the antinociceptive effects of oxycodone, a commonly used analgesics to manage SCD-related pain, in both genotypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mixed sex, 2-, 5-, and 10-month HbSS and HbAA control mice were assessed in cadre of stimulus-evoked and non-evoked functional assays. The dose-response relationship of oxycodone was evaluated in 10-month mice from both genotypes in a subset of in vivo assays. Finally, qRT-PCR was used to quantify the relative mRNA levels of opioid receptors and ligand precursors, and pro-inflammatory cytokines, from spinal cord and dorsal root ganglia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>HbSS mice displayed augmented responses in stimulus-evoked assays and deficits in non-evoked functional behaviors that overall worsened in severity over age, compared with controls. Oxycodone dose-dependently attenuated mechanical hypersensitivity and produced thermal antinociception but failed to normalize (or worsened) functional behavior. Finally, HbSS mice exhibited overall or age-dependent differences in mRNA amounts of mu and kappa opioid receptors, POMC, IL-1\u03b2, and IL-6.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study offers a comprehensive approach to investigate candidate drugs to treat SCD pain and explores biomarkers associated with the HbSS SCD mouse model. Although oxycodone ameliorated the hyper-nociceptive phenotype of HbSS mice, it failed to restore functional behavior, underscoring the need to identify novel therapeutic strategies that effectively reduce pain and restore functional behavior.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jscdis\/yoaf033","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T18:15:26Z","timestamp":1759860926000},"source":"Crossref","is-referenced-by-count":1,"title":["Behavioral and transcriptional effects of age in HbSS-BERK humanized SCD mice"],"prefix":"10.1093","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9403-2721","authenticated-orcid":false,"given":"Kennedy N","family":"Goldsborough","sequence":"first","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8400-4039","authenticated-orcid":false,"given":"Michael W","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1447-5803","authenticated-orcid":false,"given":"Bryan D","family":"McKiver","sequence":"additional","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2594-1347","authenticated-orcid":false,"given":"Karan H","family":"Muchhala","sequence":"additional","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8923-8612","authenticated-orcid":false,"given":"Molly E","family":"Sonenklar","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology\/Oncology, Virginia Commonwealth University , Richmond, VA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7921-8800","authenticated-orcid":false,"given":"Atuahene","family":"Adu-Gyamfi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8763-5670","authenticated-orcid":false,"given":"Sara M","family":"Herz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9104-7151","authenticated-orcid":false,"given":"Dawn K","family":"Jessup","sequence":"additional","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0952-9923","authenticated-orcid":false,"given":"Joyce A","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular Genetics, Virginia Commonwealth University , Richmond, VA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1423-0774","authenticated-orcid":false,"given":"Hamid I","family":"Akbarali","sequence":"additional","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8859-029X","authenticated-orcid":false,"given":"M Imad","family":"Damaj","sequence":"additional","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9381-9979","authenticated-orcid":false,"given":"Kalpna","family":"Gupta","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of California , Irvine, CA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4122-5367","authenticated-orcid":false,"given":"Wally R","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Virginia Commonwealth University , Richmond, VA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5601-985X","authenticated-orcid":false,"given":"Aron H","family":"Lichtman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology\/Toxicology, Virginia Commonwealth University , Richmond, VA 23298,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,10,7]]},"reference":[{"issue":"3","key":"2025111512311662600_yoaf033-B1","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1002\/pbc.24838","article-title":"Neuropathic pain in patients with sickle cell disease","volume":"61","author":"Brandow","year":"2014","journal-title":"Pediatr Blood Cancer"},{"issue":"3","key":"2025111512311662600_yoaf033-B2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1097\/MOH.0000000000000491","article-title":"Pain and sickle cell disease","volume":"26","author":"Aich","year":"2019","journal-title":"Curr Opin Hematol"},{"issue":"1","key":"2025111512311662600_yoaf033-B3","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1182\/hematology.2020000143","article-title":"Optimizing the management of chronic pain in sickle cell disease","volume":"2020","author":"Osunkwo","year":"2020","journal-title":"Hematology Am Soc Hematol Educ Program."},{"issue":"1-2","key":"2025111512311662600_yoaf033-B4","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.pain.2009.06.029","article-title":"Pain site frequency and location in sickle cell disease: the PiSCES project","volume":"145","author":"McClish","year":"2009","journal-title":"Pain."},{"issue":"1","key":"2025111512311662600_yoaf033-B5","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/ajh.23341","article-title":"Patients with sickle cell disease have increased sensitivity to cold and heat","volume":"88","author":"Brandow","year":"2013","journal-title":"Am J Hematol."},{"issue":"12","key":"2025111512311662600_yoaf033-B6","doi-asserted-by":"crossref","first-page":"3376","DOI":"10.1182\/blood-2010-12-327429","article-title":"Transient receptor potential vanilloid 1 mediates pain in mice with severe sickle cell disease","volume":"118","author":"Hillery","year":"2011","journal-title":"Blood"},{"issue":"3","key":"2025111512311662600_yoaf033-B7","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1182\/blood-2010-01-260372","article-title":"Pain-related behaviors and neurochemical alterations in mice expressing sickle hemoglobin: modulation by cannabinoids","volume":"116","author":"Kohli","year":"2010","journal-title":"Blood."},{"issue":"4","key":"2025111512311662600_yoaf033-B8","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1182\/blood-2005-07-2839","article-title":"Pathology of Berkeley sickle cell mice: similarities and differences with human sickle cell disease","volume":"107","author":"Manci","year":"2006","journal-title":"Blood."},{"issue":"10","key":"2025111512311662600_yoaf033-B9","doi-asserted-by":"crossref","first-page":"3953","DOI":"10.1182\/blood-2002-10-3313","article-title":"Transgenic sickle mice have vascular inflammation","volume":"101","author":"Belcher","year":"2003","journal-title":"Blood"},{"issue":"3","key":"2025111512311662600_yoaf033-B10","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1172\/JCI9225","article-title":"Hypoxia\/reoxygenation causes inflammatory response in transgenic sickle mice but not in normal mice","volume":"106","author":"Kaul","year":"2000","journal-title":"J Clin Invest"},{"issue":"3","key":"2025111512311662600_yoaf033-B11","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/ajh.25384","article-title":"Critical role of C5a in sickle cell disease","volume":"94","author":"Vercellotti","year":"2019","journal-title":"Am J Hematol"},{"key":"2025111512311662600_yoaf033-B12","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/1744-8069-8-62","article-title":"Sickle cell mice exhibit mechanical allodynia and enhanced responsiveness in light touch cutaneous mechanoreceptors","volume":"8","author":"Garrison","year":"2012","journal-title":"Mol Pain"},{"issue":"1","key":"2025111512311662600_yoaf033-B13","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1177\/1535370214544275","article-title":"Sickle cell disease in mice is associated with sensitization of sensory nerve fibers","volume":"240","author":"Kenyon","year":"2015","journal-title":"Exp Biol Med (Maywood)"},{"issue":"9","key":"2025111512311662600_yoaf033-B14","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1097\/j.pain.0000000000000966","article-title":"Sensitization of C-fiber nociceptors in mice with sickle cell disease is decreased by local inhibition of anandamide hydrolysis","volume":"158","author":"Uhelski","year":"2017","journal-title":"Pain"},{"issue":"12","key":"2025111512311662600_yoaf033-B15","doi-asserted-by":"crossref","first-page":"2476","DOI":"10.1016\/j.pain.2014.05.030","article-title":"Cold hypersensitivity increases with age in mice with sickle cell disease","volume":"155","author":"Zappia","year":"2014","journal-title":"Pain."},{"issue":"4","key":"2025111512311662600_yoaf033-B16","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1097\/j.pain.0000000000000104","article-title":"Sensitization of nociceptive spinal neurons contributes to pain in a transgenic model of sickle cell disease","volume":"156","author":"Cataldo","year":"2015","journal-title":"Pain."},{"issue":"8","key":"2025111512311662600_yoaf033-B17","doi-asserted-by":"crossref","first-page":"e0160608","DOI":"10.1371\/journal.pone.0160608","article-title":"Comparative analysis of pain behaviours in humanized mouse models of sickle cell anemia","volume":"11","author":"Lei","year":"2016","journal-title":"PLoS One."},{"issue":"1","key":"2025111512311662600_yoaf033-B18","doi-asserted-by":"crossref","first-page":"e54","DOI":"10.1002\/cpns.54","article-title":"Mouse models of pain in sickle cell disease","volume":"85","author":"Sagi","year":"2018","journal-title":"Curr Protoc Neurosci."},{"issue":"1","key":"2025111512311662600_yoaf033-B19","first-page":"553","article-title":"Neuropathic pain in sickle cell disease: measurement and management","volume":"2020","author":"Glaros","year":"2020","journal-title":"Hematology Am Soc Hematol Educ Program."},{"issue":"1","key":"2025111512311662600_yoaf033-B20","doi-asserted-by":"crossref","first-page":"67","DOI":"10.4103\/ijri.IJRI_364_18","article-title":"Upper limb peripheral neuropathy in sickle cell anemia: MR neurography appearances","volume":"29","author":"Poh","year":"2019","journal-title":"Indian J Radiol Imaging."},{"issue":"4","key":"2025111512311662600_yoaf033-B21","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1002\/mus.880140412","article-title":"Mononeuropathy in sickle cell anemia: anatomical and pathophysiological basis for its rarity","volume":"14","author":"Shields","year":"1991","journal-title":"Muscle Nerve."},{"issue":"4","key":"2025111512311662600_yoaf033-B22","doi-asserted-by":"crossref","first-page":"e765","DOI":"10.1097\/PR9.0000000000000765","article-title":"Peripheral nerve pathology in sickle cell disease mice","volume":"4","author":"Sadler","year":"2019","journal-title":"Pain Rep."},{"issue":"2","key":"2025111512311662600_yoaf033-B23","doi-asserted-by":"crossref","first-page":"73","DOI":"10.4103\/1110-161X.157865","article-title":"Trigeminal nerve electrophysiological assessment in sickle cell anemia: correlation with disease severity and radiological findings","volume":"42","author":"Gadallah","year":"2015","journal-title":"Egypt Rheumatol Rehabil."},{"issue":"7","key":"2025111512311662600_yoaf033-B24","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1111\/j.1468-1331.2009.02592.x","article-title":"Peripheral nervous system involvement in patients with sickle cell disease","volume":"16","author":"Okuyucu","year":"2009","journal-title":"Eur J Neurol."},{"issue":"1","key":"2025111512311662600_yoaf033-B25","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1089\/can.2017.0011","article-title":"The use of cannabis by patients with sickle cell disease increased the frequency of hospitalization due to vaso-occlusive crises","volume":"2","author":"Ballas","year":"2017","journal-title":"Cannabis Cannabinoid Res."},{"issue":"13","key":"2025111512311662600_yoaf033-B26","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1038\/s41386-019-0458-9","article-title":"Experimental design and analysis for consideration of sex as a biological variable","volume":"44","author":"Diester","year":"2019","journal-title":"Neuropsychopharmacology."},{"issue":"7","key":"2025111512311662600_yoaf033-B27","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1046\/j.1460-9568.1999.00641.x","article-title":"Endogenous interleukin-6 contributes to hypersensitivity to cutaneous stimuli and changes in neuropeptides associated with chronic nerve constriction in mice","volume":"11","author":"Murphy","year":"1999","journal-title":"Eur J Neurosci."},{"issue":"3","key":"2025111512311662600_yoaf033-B28","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0022-3565(25)30089-3","article-title":"Quantification of the analgesic activity of narcotic antagonists by a modified hot-plate procedure","volume":"192","author":"O\u2019Callaghan","year":"1975","journal-title":"J Pharmacol Exp Ther."},{"issue":"3","key":"2025111512311662600_yoaf033-B29","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/0304-3959(94)90023-X","article-title":"Behavioral signs of ongoing pain and cold allodynia in a rat model of neuropathic pain","volume":"59","author":"Yoon","year":"1994","journal-title":"Pain."},{"issue":"1-2","key":"2025111512311662600_yoaf033-B30","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/0165-0270(95)00049-6","article-title":"Quantitative measurement of muscle strength in the mouse","volume":"62","author":"Smith","year":"1995","journal-title":"J Neurosci Methods."},{"issue":"76","key":"2025111512311662600_yoaf033-B31","doi-asserted-by":"crossref","DOI":"10.3791\/2610-v","article-title":"Measuring the strength of mice","author":"Deacon","year":"2013","journal-title":"J Vis Exp"},{"issue":"4","key":"2025111512311662600_yoaf033-B32","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1002\/9780470942390.mo140295","article-title":"Voluntary wheel running in mice","volume":"5","author":"Goh","year":"2015","journal-title":"Curr Protoc Mouse Biol."},{"issue":"12","key":"2025111512311662600_yoaf033-B33","doi-asserted-by":"crossref","first-page":"e0261662","DOI":"10.1371\/journal.pone.0261662","article-title":"Voluntary wheel running behaviour as a tool to assess the severity in a mouse pancreatic cancer model","volume":"16","author":"Weegh","year":"2021","journal-title":"PLoS One."},{"key":"2025111512311662600_yoaf033-B34","doi-asserted-by":"crossref","first-page":"124","DOI":"10.3389\/fnbeh.2018.00124","article-title":"Deficits in burrowing behaviors are associated with mouse models of neuropathic but not inflammatory pain or migraine","volume":"12","author":"Shepherd","year":"2018","journal-title":"Front Behav Neurosci."},{"issue":"59","key":"2025111512311662600_yoaf033-B35","first-page":"e2607","article-title":"Assessing burrowing, nest construction, and hoarding in mice","volume":"2607","author":"Deacon","year":"2012","journal-title":"J Vis Exp."},{"issue":"6","key":"2025111512311662600_yoaf033-B36","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1097\/j.pain.0000000000000171","article-title":"Effects of ketoprofen, morphine, and kappa opioids on pain-related depression of nesting in mice","volume":"156","author":"Negus","year":"2015","journal-title":"Pain."},{"key":"2025111512311662600_yoaf033-B37","doi-asserted-by":"crossref","first-page":"105510","DOI":"10.1016\/j.jpain.2025.105510","article-title":"Paclitaxel-induced neuroinflammation after systemic administration in male and female mice","volume":"35","author":"Caillaud","year":"2025","journal-title":"J Pain."},{"key":"2025111512311662600_yoaf033-B38","doi-asserted-by":"crossref","first-page":"174007","DOI":"10.1016\/j.ejphar.2021.174007","article-title":"Role of \u03b2-arrestin-2 in short- and long-term opioid tolerance in the dorsal root ganglia","volume":"899","author":"Muchhala","year":"2021","journal-title":"Eur J Pharmacol."},{"issue":"49","key":"2025111512311662600_yoaf033-B39","doi-asserted-by":"crossref","first-page":"34241","DOI":"10.1074\/jbc.M114.570101","article-title":"Chemical structure and morphology of dorsal root ganglion neurons from naive and inflamed mice","volume":"289","author":"Barabas","year":"2014","journal-title":"J Biol Chem."},{"issue":"4","key":"2025111512311662600_yoaf033-B40","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s00424-021-02653-9","article-title":"Neuronal allodynic mechanisms of Slc7a5 (LAT1) in the spared nerve injury rodent model of neuropathic pain","volume":"474","author":"Goins","year":"2022","journal-title":"Pflugers Arch."},{"issue":"5240","key":"2025111512311662600_yoaf033-B41","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1126\/science.270.5240.1366","article-title":"Selective opioid inhibition of small nociceptive neurons","volume":"270","author":"Taddese","year":"1995","journal-title":"Science."},{"issue":"3","key":"2025111512311662600_yoaf033-B42","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1037\/1064-1297.12.3.163","article-title":"Discriminative stimulus, reinforcing, physical dependence, and antinociceptive effects of oxycodone in mice, rats, and rhesus monkeys","volume":"12","author":"Beardsley","year":"2004","journal-title":"Exp Clin Psychopharmacol"},{"issue":"10","key":"2025111512311662600_yoaf033-B43","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1111\/pme.12413","article-title":"The role of the dorsal root ganglion in the development of neuropathic pain","volume":"15","author":"Krames","year":"2014","journal-title":"Pain Med."},{"key":"2025111512311662600_yoaf033-B44","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.nbd.2015.10.004","article-title":"Cognitive and behavior deficits in sickle cell mice are associated with profound neuropathologic changes in hippocampus and cerebellum","volume":"85","author":"Wang","year":"2016","journal-title":"Neurobiol Dis"},{"key":"2025111512311662600_yoaf033-B45","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/B978-1-4160-2392-0.50017-0","volume-title":"Complications in Regional Anesthesia & Pain Medicine","author":"Hebl","year":"2007"},{"key":"2025111512311662600_yoaf033-B46","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.neuroscience.2019.03.041","article-title":"Pubertal hormones mediate sex differences in levels of myelin basic protein in the orbitofrontal cortex of adult rats","volume":"406","author":"Darling","year":"2019","journal-title":"Neuroscience."},{"issue":"1","key":"2025111512311662600_yoaf033-B47","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1124\/jpet.115.229971","article-title":"The selective monoacylglycerol lipase inhibitor MJN110 produces opioid-sparing effects in a mouse neuropathic pain model","volume":"357","author":"Wilkerson","year":"2016","journal-title":"J Pharmacol Exp Ther."},{"issue":"5","key":"2025111512311662600_yoaf033-B48","doi-asserted-by":"crossref","first-page":"566","DOI":"10.3324\/haematol.2015.136523","article-title":"Cannabinoid receptor-specific mechanisms to alleviate pain in sickle cell anemia via inhibition of mast cell activation and neurogenic inflammation","volume":"101","author":"Vincent","year":"2016","journal-title":"Haematologica."},{"key":"2025111512311662600_yoaf033-B49","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.3389\/fphar.2018.01388","article-title":"Advances in achieving opioid analgesia without side effects","volume":"9","author":"Machelska","year":"2018","journal-title":"Front Pharmacol."},{"key":"2025111512311662600_yoaf033-B50","doi-asserted-by":"crossref","first-page":"170051","DOI":"10.1038\/sdata.2017.51","article-title":"Transcriptomic analysis of gene signatures associated with sickle pain","volume":"4","author":"Paul","year":"2017","journal-title":"Sci Data"},{"issue":"1","key":"2025111512311662600_yoaf033-B51","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1684\/ecn.2013.0328","article-title":"Altered levels of pro-inflammatory cytokines in sickle cell disease patients during vaso-occlusive crises and the steady state condition","volume":"24","author":"Keikhaei","year":"2013","journal-title":"Eur Cytokine Netw"},{"issue":"1","key":"2025111512311662600_yoaf033-B52","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.cyto.2014.11.030","article-title":"Serum IL-6, IL-10, and TNF\u03b1 levels in pediatric sickle cell disease patients during vasoocclusive crisis and steady state condition","volume":"72","author":"Sarray","year":"2015","journal-title":"Cytokine"},{"issue":"1","key":"2025111512311662600_yoaf033-B53","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.cyto.2009.10.002","article-title":"Plasma interleukin-1beta concentration is associated with stroke in sickle cell disease","volume":"49","author":"Asare","year":"2010","journal-title":"Cytokine"},{"key":"2025111512311662600_yoaf033-B54","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.cyto.2016.03.016","article-title":"Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell","volume":"83","author":"Pitanga","year":"2016","journal-title":"Cytokine"},{"issue":"3","key":"2025111512311662600_yoaf033-B55","first-page":"725","article-title":"Targeting P-selectin and interleukin-1\u03b2 in mice with sickle cell disease: effects on vaso-occlusion, liver injury and organ iron deposition","volume":"110","author":"Gotardo","year":"2025","journal-title":"Haematologica"},{"key":"2025111512311662600_yoaf033-B56","doi-asserted-by":"crossref","first-page":"34493","DOI":"10.1038\/srep34493","article-title":"Electroacupuncture in conscious free-moving mice reduces pain by ameliorating peripheral and central nociceptive mechanisms","volume":"6","author":"Wang","year":"2016","journal-title":"Sci Rep"},{"issue":"17","key":"2025111512311662600_yoaf033-B57","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1182\/blood.2021013674","article-title":"A randomized, placebo-controlled, double-blind trial of canakinumab in children and young adults with sickle cell anemia","volume":"139","author":"Rees","year":"2022","journal-title":"Blood"},{"issue":"8","key":"2025111512311662600_yoaf033-B58","doi-asserted-by":"crossref","first-page":"e331","DOI":"10.3324\/haematol.2018.208322","article-title":"Diet and gender influence survival of transgenic berkley sickle cell mice","volume":"104","author":"Jahagirdar","year":"2019","journal-title":"Haematologica."},{"issue":"6","key":"2025111512311662600_yoaf033-B59","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1182\/bloodadvances.2018024299","article-title":"Effect of chronic opioid therapy on pain and survival in a humanized mouse model of sickle cell disease","volume":"3","author":"Tran","year":"2019","journal-title":"Blood Adv."},{"key":"2025111512311662600_yoaf033-B60","doi-asserted-by":"crossref","first-page":"2798","DOI":"10.1097\/j.pain.0000000000000704","article-title":"CaMKII\u00e1 underlies spontaneous and evoked pain behaviors in Berkeley sickle cell transgenic mice","volume":"157","author":"He","year":"2016","journal-title":"Pain"}],"container-title":["Journal of Sickle Cell Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jscd\/advance-article-pdf\/doi\/10.1093\/jscdis\/yoaf033\/64539552\/yoaf033.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jscd\/article-pdf\/2\/1\/yoaf033\/64539552\/yoaf033.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jscd\/article-pdf\/2\/1\/yoaf033\/64539552\/yoaf033.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T17:31:28Z","timestamp":1763227888000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jscd\/article\/doi\/10.1093\/jscdis\/yoaf033\/8277012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1,17]]}},"URL":"https:\/\/doi.org\/10.1093\/jscdis\/yoaf033","relation":{},"ISSN":["3029-0473"],"issn-type":[{"value":"3029-0473","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025]]},"published":{"date-parts":[[2025]]},"article-number":"yoaf033"}}