{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T21:04:53Z","timestamp":1774559093324,"version":"3.50.1"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":68,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Office of Research, University of California"},{"name":"National Institute of Health","award":["R01 HL147562"],"award-info":[{"award-number":["R01 HL147562"]}]},{"name":"National Institute of Health","award":["U24 AT012868"],"award-info":[{"award-number":["U24 AT012868"]}]},{"name":"National Institute of Health","award":["3R01HL147562-03S(SK)"],"award-info":[{"award-number":["3R01HL147562-03S(SK)"]}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CA263806"],"award-info":[{"award-number":["CA263806"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Stroke in sickle cell disease (SCD) is often attributed to large vessel involvement in the disorder, whereas the contribution of cerebral microvascular disease has been less explored. In this study, we investigated the formation of cerebral microvascular lesions and the involvement of mast cells in a humanized SCD mouse model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We studied hemorrhagic microvascular disease in a well-characterized mouse model of humanized transgenic sickle (HbSS-BERK) expressing &amp;gt;99% human sickle hemoglobin (HbS) and a control (HbAA-BERK) mouse model expressing normal human hemoglobin A (HbA). Mouse brains were analyzed by Prussian blue staining to detect cerebral microhemorrhage (CMH) formation. Mast cell identification was performed by toluidine blue staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>SCD brain sections exhibited approximately 86% more CMH than controls (mean \u00b1 SE of 1.17\u2009\u00b1\u20090.22 vs. 0.63\u2009\u00b1\u20090.13 number\/cm2, P\u2009=\u2009.02). Mast cells were positively correlated with CMH number in SCD mice (Spearman r\u2009=\u20090.42, P\u2009&amp;lt;\u2009.05), but not in control mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>SCD mice demonstrated significantly increased CMH load compared with control mice, and SCD microhemorrhages were associated with the number of mast cells. These findings highlight the significance of cerebral microvascular disease in SCD and imply that cerebral mast cells may be a novel therapeutic target in SCD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jscdis\/yoag015","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T04:23:56Z","timestamp":1773116636000},"source":"Crossref","is-referenced-by-count":0,"title":["Cerebral microhemorrhages in a mouse model of sickle cell disease"],"prefix":"10.1093","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6466-9921","authenticated-orcid":false,"given":"Yu-Han","family":"Hung","sequence":"first","affiliation":[{"name":"Department of Neurology, University of California , Irvine, Orange, CA, 92868,","place":["United 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Orange, CA, 92868,","place":["United States"]},{"name":"Department of Neurology, Veterans Health Service Medical Center , Seoul, 05368,","place":["South Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4106-3334","authenticated-orcid":false,"given":"Annlia","family":"Paganini-Hill","sequence":"additional","affiliation":[{"name":"Beckman Laser Institute, University of California , Irvine, Irvine, CA, 92697,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6870-4480","authenticated-orcid":false,"given":"David H","family":"Cribbs","sequence":"additional","affiliation":[{"name":"Institute for Memory Impairments and Neurological Disorders, University of California , Irvine, Irvine, CA, 92697,","place":["United 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