{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:26:53Z","timestamp":1792794413905,"version":"4.5.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T00:00:00Z","timestamp":1726358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"National Institute of Diabetes and Digestive and Kidney Diseases (National Institutes of Health","award":["R01 DK101536"],"award-info":[{"award-number":["R01 DK101536"]}],"id":[{"id":"https:\/\/ror.org\/00adh9b73","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cavernous nerve (CN) injury, which occurs in prostatectomy and diabetic cases, initiates penile remodeling, including smooth muscle apoptosis and increased collagen in the corpora cavernosa, which are underlying causes of erectile dysfunction. Sonic hedgehog (SHH) is a critical regulator of penile smooth muscle, and SHH treatment suppresses corpora cavernosa remodeling that occurs with CN injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>We examine if SHH treatment by peptide amphiphile (PA) in the first week after CN injury is sufficient to prevent long-term penis remodeling and if apoptosis inhibitors also suppress penile remodeling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bilateral CN crush was performed on adult Sprague-Dawley rats (P115-120) that underwent 1 of 3 treatments with novel extended-release nanofiber PA hydrogels for delivery: SHH protein (n\u2009=\u200910), mouse serum albumin protein (control, n\u2009=\u20097), or caspase 3 inhibitor (AC-DEVD-CHO, n\u2009=\u200910). Rats were sacrificed after 18 to 24\u00a0days. Additional rats underwent CN injury (n\u2009=\u20096) or CN injury and SHH PA treatment for 2 and 4\u00a0days (n\u2009=\u20098) and included sham controls (n\u2009=\u20093) and nonsurgery controls (n\u2009=\u20093).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Outcomes<\/jats:title>\n                    <jats:p>Trichrome stain, hydroxyproline assay, and Western analysis for \u03b1-actin (smooth muscle) and GAPDH were performed to examine smooth muscle retention and collagen abundance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Smooth muscle decreased with CN injury. Corpora cavernosa showed increased smooth muscle at 2, 4, and 24\u00a0days after CN injury with SHH PA treatment in comparison with mouse serum albumin treatment among CN-injured controls. Caspase 3\u2013inhibited penis demonstrated little smooth muscle preservation. Collagen was decreased 23% with SHH PA treatment (P\u2009&amp;lt;\u2009.001) at 18 to 24\u00a0days after CN injury. Collagen was unchanged with caspase 3 inhibitor treatment (P\u2009&amp;gt;\u2009.99).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Translation<\/jats:title>\n                    <jats:p>It is important to know that treatments given at the time of CN injury have a sustained effect on preserving penile architecture and thus erectile function, making them valuable for clinical translation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Strengths and Limitations<\/jats:title>\n                    <jats:p>SHH PA treatment preserves penile smooth muscle after CN injury. Time points past 24\u00a0days were not examined, although penile remodeling takes place acutely after CN injury. Measurement of erectile function was not examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>SHH treatment by PA in the first week after CN injury is sufficient to suppress penile remodeling and to preserve penile smooth muscle over time, which is critical to prevent development of erectile dysfunction. There is a difference in the corpora cavernosa smooth muscle from proximal to distal in the penis of the Sprague-Dawley rat model. It is critical when examining therapy efficacy to ensure that comparable regions of the penis are analyzed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Statement of Significance<\/jats:title>\n                    <jats:p>In this study, we examine if suppression of apoptosis in penile smooth muscle in the first week after cavernous nerve injury is sufficient to preserve smooth muscle long-term.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jsxmed\/qdae116","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T18:22:17Z","timestamp":1724696537000},"page":"986-993","source":"Crossref","is-referenced-by-count":3,"title":["Sonic hedgehog suppresses penile remodeling after cavernous nerve injury and sustains long-term normal penis morphology"],"prefix":"10.1093","volume":"21","author":[{"given":"Jiangping","family":"Deng","sequence":"first","affiliation":[{"name":"University of Illinois at Chicago Department of Urology, , Chicago, IL 60612,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Triko","sequence":"additional","affiliation":[{"name":"University of Illinois at Chicago Department of Urology, , Chicago, IL 60612,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A","family":"Harrington","sequence":"additional","affiliation":[{"name":"UTHealth, The University of Texas Health Science Center at Houston Department of Diagnostic and Biomedical Sciences, , Houston, TX 77054,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin T","family":"McVary","sequence":"additional","affiliation":[{"name":"Loyola University Stritch School of Medicine Department of Urology, , Maywood, IL 60153,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol A","family":"Podlasek","sequence":"additional","affiliation":[{"name":"University of Illinois at Chicago Departments of Urology, Physiology, Bioengineering, and Biochemistry, , Chicago, IL 60612,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,15]]},"reference":[{"issue":"2","key":"2024110500214215900_ref1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3816\/CGC.2004.n.017","article-title":"Current concepts in the management of erectile dysfunction in men with prostate cancer","volume":"3","author":"Kendirci","year":"2004","journal-title":"Clin Prostate Cancer"},{"issue":"9","key":"2024110500214215900_ref2","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/S1470-2045(11)70162-0","article-title":"SPCG-4 Investigators. 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