{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T14:43:59Z","timestamp":1767624239618,"version":"3.48.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Peyronie\u2019s disease is characterized by penile curvature, sexual dysfunction, and pain with sexual activity, and its pathogenesis is thought to involve several molecular mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>The aim of the present study was to identify differential gene expression in the tunica albuginea (TA) tissue and corpora cavernosa (CC) tissue of a group of patients with Peyronie\u2019s Disease relative to a control group of patients with erectile dysfunction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Tissue from the TA and CC was collected from patients at the time of surgery. RNA sequencing analysis was performed on total RNA isolated from penile tissue. Gene ontology analyses were then performed to identify which molecular and biologic processes were associated with the differentially expressed genes. Clinical demographic factors were also collected from chart review of patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Outcomes<\/jats:title>\n                    <jats:p>Analysis revealed a distinct gene expression profile in the TA of Peyronie\u2019s patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 58 patients were included in the study; 57 had CC for RNA isolation and 30 had TA for RNA isolation. Among those with tunica, 15 had Peyronie\u2019s and 15 did not. This analysis identified 22 significantly differentially expressed genes between the groups. Bioinformatics analyses revealed that differentially expressed genes were implicated in immune and apoptotic processes. For the corpora population only two genes were found to be differentially expressed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Implications<\/jats:title>\n                    <jats:p>Further understanding of the genes implicated in Peyronie\u2019s could lead to additional treatments targeted at the prevention and progression of this disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Strengths &amp; Limitations<\/jats:title>\n                    <jats:p>This study uses next-generation RNA sequencing to analyze whole-gene expression in a relatively large cohort, identifying novel genes involved in Peyronie\u2019s disease pathogenesis. Limitations include the use of formalin-fixed tissue and potential confounding due to differences in erectile function between Peyronie\u2019s and control groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Distinct genes were differentially expressed in the TA tissue of patients with Peyronie\u2019s disease when compared with controls and were largely implicated in immune and apoptotic processes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jsxmed\/qdaf352","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T13:10:33Z","timestamp":1765285833000},"source":"Crossref","is-referenced-by-count":0,"title":["Differential genetic expression in the tunica albuginea tissue of patients with Peyronie\u2019s disease"],"prefix":"10.1093","volume":"23","author":[{"given":"Samantha R","family":"Freeman","sequence":"first","affiliation":[{"name":"Department of Urology, Lahey Hospital & Medical Center , Burlington, MA 01803 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua P","family":"Hayden","sequence":"additional","affiliation":[{"name":"Department of Urology, Lahey Hospital & Medical Center , Burlington, MA 01803 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailey","family":"Hooper","sequence":"additional","affiliation":[{"name":"Department of Translational Research, Lahey Hospital & Medical Center , Burlington, MA 01803 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Travis","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Department of Translational Research, Lahey Hospital & Medical Center , Burlington, MA 01803 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Perrino","sequence":"additional","affiliation":[{"name":"Department of Pathology, Lahey Hospital & Medical Center , Burlington, MA 01803 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Rieger-Christ","sequence":"additional","affiliation":[{"name":"Department of Translational Research, Lahey Hospital & Medical Center , Burlington, MA 01803 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex J","family":"Vanni","sequence":"additional","affiliation":[{"name":"Department of Urology, Lahey Hospital & Medical Center , Burlington, MA 01803 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"issue":"1","key":"2026010506163895800_ref1","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/S0022-5347(01)65360-6","article-title":"The microscopic pathology of Peyronie's disease","volume":"157","author":"Davis","year":"1997","journal-title":"J Urol"},{"issue":"3","key":"2026010506163895800_ref2","doi-asserted-by":"publisher","first-page":"399","DOI":"10.5534\/wjmh.200065","article-title":"The natural history of Peyronie's disease","volume":"39","author":"Di Maida","year":"2021","journal-title":"World J Mens Health"},{"issue":"2","key":"2026010506163895800_ref3","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.esxm.2019.11.002","article-title":"Prevalence, risk factors, and erectile dysfunction associated with Peyronie's disease among men seeking urological care","volume":"8","author":"Segundo","year":"2020","journal-title":"Sex Med"},{"issue":"5","key":"2026010506163895800_ref4","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1159\/000049830","article-title":"Peyronie\u2019s disease: prevalence and association with cigarette smoking. A multicenter population-based study in men aged 50-69 years","volume":"40","author":"La Pera","year":"2001","journal-title":"Eur Urol"},{"issue":"3","key":"2026010506163895800_ref5","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1016\/j.eururo.2005.10.026","article-title":"Prevalence of Peyronie's disease among patients with erectile dysfunction","volume":"49","author":"El-Sakka","year":"2006","journal-title":"Eur Urol"},{"issue":"3","key":"2026010506163895800_ref6","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1016\/j.juro.2015.05.098","article-title":"Peyronie's disease: AUA guideline","volume":"194","author":"Nehra","year":"2015","journal-title":"J Urol"},{"issue":"1","key":"2026010506163895800_ref7","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.juro.2013.01.087","article-title":"Clinical efficacy, safety and tolerability of collagenase clostridium histolyticum for the treatment of peyronie disease in 2 large double-blind, randomized, placebo controlled phase 3 studies","volume":"190","author":"Gelbard","year":"2013","journal-title":"J Urol"},{"issue":"4 Pt 1","key":"2026010506163895800_ref8","doi-asserted-by":"publisher","first-page":"1051","DOI":"10.1016\/j.juro.2015.10.065","article-title":"Restoration of penile function and patient satisfaction with intralesional collagenase clostridium histolyticum injection for Peyronie's disease","volume":"195","author":"Ziegelmann","year":"2016","journal-title":"J Urol"},{"issue":"2","key":"2026010506163895800_ref9","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1016\/j.sxmr.2017.07.011","article-title":"Intralesional injection of collagenase clostridium histolyticum may increase the risk of late-onset penile fracture","volume":"6","author":"Beilan","year":"2018","journal-title":"Sex Med Rev"},{"issue":"10","key":"2026010506163895800_ref10","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1007\/s11934-014-0446-x","article-title":"Surgical management of Peyronie's disease","volume":"15","author":"Zaid","year":"2014","journal-title":"Curr Urol Rep"},{"issue":"6","key":"2026010506163895800_ref11","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1038\/ncpuro0201","article-title":"Mechanisms of disease: new insights into the cellular and molecular pathology of Peyronie's disease","volume":"2","author":"Gonzalez-Cadavid","year":"2005","journal-title":"Nat Clin Pract Urol"},{"issue":"2","key":"2026010506163895800_ref12","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1038\/s41443-017-0015-1","article-title":"Extracellular matrix alterations after blood instillation in tunica albuginea of rats","volume":"30","author":"Cohen","year":"2018","journal-title":"Int J Impot Res"},{"issue":"4","key":"2026010506163895800_ref13","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1111\/bju.14638","article-title":"Simvastatin and the rho-kinase inhibitor Y-27632 prevent myofibroblast transformation in Peyronie's disease-derived fibroblasts via inhibition of YAP\/TAZ nuclear translocation","volume":"123","author":"Milenkovic","year":"2019","journal-title":"BJU Int"},{"issue":"2","key":"2026010506163895800_ref14","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.eururo.2018.10.014","article-title":"Antifibrotic synergy between phosphodiesterase type 5 inhibitors and selective oestrogen receptor modulators in Peyronie\u2019s disease models","volume":"75","author":"Ilg","year":"2019","journal-title":"Eur Urol"},{"issue":"5","key":"2026010506163895800_ref15","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1038\/sj.ijir.3900876","article-title":"Peyronie's disease: an anatomically-based hypothesis and beyond","volume":"14","author":"Lue","year":"2002","journal-title":"Int J Impot Res"},{"issue":"2","key":"2026010506163895800_ref16","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1002\/path.2277","article-title":"Cellular and molecular mechanisms of fibrosis","volume":"214","author":"Wynn","year":"2008","journal-title":"J Pathol"},{"issue":"3","key":"2026010506163895800_ref17","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1016\/s0090-4295(01)01578-3","article-title":"Gene expression profiles in the Peyronie's disease plaque","volume":"59","author":"Magee","year":"2002","journal-title":"Urology."},{"issue":"6","key":"2026010506163895800_ref18","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1007\/s11934-004-0074-y","article-title":"New discoveries in the basic science understanding of Peyronie's disease","volume":"5","author":"Jalkut","year":"2004","journal-title":"Curr Urol Rep"},{"key":"2026010506163895800_ref19","doi-asserted-by":"publisher","first-page":"100990","DOI":"10.1016\/j.mgene.2021.100990","article-title":"MicroRNAs based regulation of cytokine regulating immune expressed genes and their transcription factors in COVID-19","volume":"31","author":"Khokhar","year":"2022","journal-title":"Meta Gene"},{"issue":"11","key":"2026010506163895800_ref20","doi-asserted-by":"publisher","first-page":"e14219","DOI":"10.1111\/and.14219","article-title":"Peyronie\u2019s disease in a patient after COVID-19 infection: a case report","volume":"53","author":"Rainer","year":"2021","journal-title":"Andrologia."},{"issue":"11","key":"2026010506163895800_ref21","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19113561","article-title":"IL-24 promotes apoptosis through cAMP-dependent PKA pathways in human breast cancer cells","volume":"19","author":"Persaud","year":"2018","journal-title":"Int J Mol Sci"},{"issue":"11","key":"2026010506163895800_ref22","doi-asserted-by":"publisher","first-page":"2436","DOI":"10.4103\/1673-5374.371368","article-title":"Promotes inflammation and neuronal apoptosis in cerebral ischemia\/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation","volume":"18","author":"Qiu","year":"2023","journal-title":"Neural Regen Res"},{"issue":"5","key":"2026010506163895800_ref23","first-page":"607","article-title":"Comparison of apoptotic gene expression profiles between Peyronie's disease plaque and tunica albuginea","volume":"21","author":"Zorba","year":"2012","journal-title":"Adv Clin Exp Med"},{"issue":"Suppl 5","key":"2026010506163895800_ref24","doi-asserted-by":"publisher","first-page":"S21","DOI":"10.1038\/sj.ijir.3900771","article-title":"Perturbation of cell cycle regulators in Peyronie's disease","volume":"13","author":"Mulhall","year":"2001","journal-title":"Int J Impot Res"},{"issue":"6861","key":"2026010506163895800_ref25","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1038\/35104560","article-title":"Induction of gadd45beta by NF-kappaB downregulates pro-apoptotic JNK signalling","volume":"414","author":"De Smaele","year":"2001","journal-title":"Nature."},{"issue":"33","key":"2026010506163895800_ref26","doi-asserted-by":"publisher","first-page":"25500","DOI":"10.1074\/jbc.M109.091413","article-title":"Gadd45b mediates Fas-induced apoptosis by enhancing the interaction between p38 and retinoblastoma tumor suppressor","volume":"285","author":"Cho","year":"2010","journal-title":"J Biol Chem"},{"issue":"4","key":"2026010506163895800_ref27","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1038\/s41419-022-04804-x","article-title":"Antiapoptotic protein FAIM2 is targeted by miR-3202, and DUX4 via TRIM21, leading to cell death and defective myogenesis","volume":"13","author":"Soliman","year":"2022","journal-title":"Cell Death Dis"},{"issue":"1","key":"2026010506163895800_ref28","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/j.eururo.2006.05.002","article-title":"Alterations in the transforming growth factor (TGF)-beta pathway as a potential factor in the pathogenesis of Peyronie's disease","volume":"51","author":"Haag","year":"2007","journal-title":"Eur Urol"},{"issue":"4","key":"2026010506163895800_ref29","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1016\/j.jare.2017.06.004","article-title":"Extracellular matrix alterations in the Peyronie's disease","volume":"8","author":"Watanabe","year":"2017","journal-title":"J Adv Res"},{"issue":"4","key":"2026010506163895800_ref30","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1016\/S0022-5347(01)64223-X","article-title":"Peyronie's disease is associated with an increase in transforming growth factor-beta protein expression","volume":"158","author":"El-Sakka","year":"1997","journal-title":"J Urol"}],"container-title":["The Journal of Sexual Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jsm\/article-pdf\/23\/1\/qdaf352\/66100218\/qdaf352.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jsm\/article-pdf\/23\/1\/qdaf352\/66100218\/qdaf352.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T11:16:45Z","timestamp":1767611805000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jsm\/article\/doi\/10.1093\/jsxmed\/qdaf352\/8400387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,22]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1,5]]}},"URL":"https:\/\/doi.org\/10.1093\/jsxmed\/qdaf352","relation":{},"ISSN":["1743-6095","1743-6109"],"issn-type":[{"type":"print","value":"1743-6095"},{"type":"electronic","value":"1743-6109"}],"subject":[],"published-other":{"date-parts":[[2026,1]]},"published":{"date-parts":[[2025,12,22]]},"article-number":"qdaf352"}}