{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T15:08:20Z","timestamp":1789398500268,"version":"build-2803163510"},"reference-count":20,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Horizon Europe program through the PREPARE-TID","award":["101137132"],"award-info":[{"award-number":["101137132"]}]},{"name":"MUR PNRR Extended Partnership Initiative on Emerging Infectious Diseases","award":["PE00000007, INF-ACT"],"award-info":[{"award-number":["PE00000007, INF-ACT"]}]},{"name":"Italian Ministry of Health \u2018Fondi Ricerca Corrente\u2019 to IRCCS Sacro Cuore Don Calabria Hospital","award":["L1P3"],"award-info":[{"award-number":["L1P3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Chikungunya virus (CHIKV), primarily transmitted by Aedes mosquitoes, has increasingly caused autochthonous outbreaks in temperate regions, including Italy. In 2025, a local CHIKV outbreak occurred in Verona province, Veneto Region. We report the findings of integrated epidemiological, virological and entomological investigations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In 2025, a dedicated surveillance protocol was implemented to assess local transmission of arboviral infections in Verona province, enabling early detection of a local CHIKV circulation. Following notification of the index case, investigations were conducted in accordance with the national and regional arbovirus surveillance plans, including comprehensive epidemiological, virological and entomological analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The index case was identified on 6 August 2025, with retrospective detection of the first case with symptom onset on 31 July; the primary case was not identified. As of 15 October, 69 cases were reported (62 confirmed, 7 probable), with a mean age of 55\u00a0years; 91.3% had identifiable epidemiological links, mainly clustered in two municipalities of the Verona province. Most cases (76.8%) occurred between mid-August and mid-September. The outbreak lasted approximately 15 weeks, from the end of July to the mid of October. CHIKV-RNA was detectable in whole blood and, less frequently, urine only within the first 5 days after symptom onset, with higher viral loads in blood. CHIKV-specific IgM antibodies were detectable from Days 4\u20135 and IgG from Days 7\u201310. A total of 225 Aedes albopictus specimens were collected, all testing negative for CHIKV. Phylogenetic analysis revealed a highly homogeneous, monophyletic cluster within the East-Central-South African genotype, closely related to strains from the 2024\u20132025 R\u00e9union outbreak.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This outbreak underscores the value of dedicated surveillance protocols for early detection of febrile\/suspected arboviral cases, while also highlighting key vulnerabilities, namely, an undetected primary case and negative entomological findings, pointing to the need for lower testing thresholds and earlier, risk-based vector sampling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jtm\/taag079","type":"journal-article","created":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T17:40:20Z","timestamp":1788025220000},"source":"Crossref","is-referenced-by-count":0,"title":["Autochthonous chikungunya virus outbreak in Verona province, Italy, 2025"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9819-236X","authenticated-orcid":false,"given":"Concetta","family":"Castilletti","sequence":"first","affiliation":[{"name":"Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital , Negrar di Valpolicella, Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Ancillotti","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Unit, Department of Medical Sciences, University Hospital of Siena , Siena ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital , Negrar di Valpolicella, Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Accordini","sequence":"additional","affiliation":[{"name":"Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital , Negrar di Valpolicella, Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Deiana","sequence":"additional","affiliation":[{"name":"Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital , Negrar di Valpolicella, Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuele","family":"Cheri","sequence":"additional","affiliation":[{"name":"Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital , Negrar di Valpolicella, Verona 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Life Science Research, Department of Public Health, Experimental and Forensic Medicine, University of Pavia , Pavia ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Matucci","sequence":"additional","affiliation":[{"name":"Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital , Negrar di Valpolicella, Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Cubi","sequence":"additional","affiliation":[{"name":"Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital , Negrar di Valpolicella, Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Zanchi","sequence":"additional","affiliation":[{"name":"Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital , Negrar di Valpolicella, Verona 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