{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T11:28:43Z","timestamp":1770550123213,"version":"3.49.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":32,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>No consensus exists regarding the monitoring and therapeutic approach to adrenal tumors (ATs) discovered incidentally by diagnostic imaging, when standard endocrine testing yields negative results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>To evaluate tissue concentrations of adrenocortical steroids in hormonally silent adrenocortical tumors (SATs) in dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Fourteen dogs with SATs (12 unilateral, 2 bilateral), 11 dogs with cortisol-secreting adrenocortical tumors (cs-ACTs) and 10 healthy dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Observational study. Diagnosis of SAT was based on finding an AT on diagnostic imaging, negative endocrine function tests, and histopathological and immunohistochemical confirmation. Adrenocortical steroid tissue concentrations were measured by liquid chromatography with tandem mass spectrometry and compared between SATs, cs-ACTs, and normal adrenals (NAs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Hormonally silent adrenocortical tumors exhibited higher median tissue cortisol (3.62\u00a0ng\/mg, range 0.05\u201318.1) and 21-deoxycortisol (0.08\u00a0ng\/mg, range 0.00\u20130.44) concentrations than NAs (cortisol 0.38\u00a0ng\/mg, range 0.01\u20131.90; 21-deoxycortisol 0.01\u00a0ng\/mg, range 0.00\u20130.03; P\u00a0=\u00a0.04 and P\u00a0=\u00a0.001, respectively), and these concentrations were not significantly different between SATs and cs-ACTs. Furthermore, SATs\u2019 median tissue concentrations of mineralocorticoid precursors corticosterone (2.15\u00a0ng\/mg, range 0.01\u201314.1) and 18-OH-corticosterone (0.70\u00a0ng\/mg, range 0.00\u20134.89) were higher than in NAs (respectively 0.19\u00a0ng\/mg [range 0.14\u20130.54] and 0.05\u00a0ng\/mg [range 0.01\u20130.33]; both P\u00a0=\u00a0.01) and not different when compared to cs-ACTs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and clinical importance<\/jats:title>\n                    <jats:p>This study on tissue metabolomics in ATs in dogs demonstrates comparable tissue concentrations of specific glucocorticoids and mineralocorticoids in SATs and cs-ACTs. This implies that some SATs are not hormonally silent, prompting further studies on diagnostics, treatment, and monitoring recommendations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jvimsj\/aalaf087","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T12:14:32Z","timestamp":1770034472000},"source":"Crossref","is-referenced-by-count":0,"title":["Glucocorticoid and mineralocorticoid production in hormonally silent adrenocortical tumor tissue in dogs"],"prefix":"10.1093","volume":"40","author":[{"given":"Kirsten L","family":"van Bokhorst","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University , 3584 CM Utrecht ,","place":["The Netherlands"]},{"name":"IVC Evidensia , 2992 LC Barendrecht ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marit F","family":"van den 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,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Corsini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Parma , 43100 Parma PR ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Golinelli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna , 40064 Ozzano dell'Emilia BO ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6932-333X","authenticated-orcid":false,"given":"Nicole","family":"Bechmann","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry and Laboratory Medicine, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universit\u00e4t Dresden , 01307 Dresden 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