{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:49:48Z","timestamp":1777870188978,"version":"3.51.4"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":60,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hospital for Small Animals Small Grants program"},{"DOI":"10.13039\/501100000848","name":"University of Edinburgh","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000848","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Brucellosis in dogs, caused by Brucella canis, is a zoonotic disease often underdiagnosed because of diagnostic challenges and subclinical infection. Bacterial culture, the reference standard, is impractical and has limited sensitivity because of intermittent or low-grade bacteremia. Accurate point-of-care (POC) testing could facilitate timely management and infection control.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>Evaluate the diagnostic performance of 3 POC lateral flow assays for detection of B. canis antibodies in dogs, using the Canine Brucella Multiplex (CBM) assay as the reference serologic assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Fifty serum samples from 50 client-owned dogs submitted for B. canis serology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Samples were classified as CBM-positive (n\u00a0=\u00a010; culture-positive dogs) or CBM-negative (n\u00a0=\u00a040; healthy dogs undergoing export testing). Assays were run in duplicate and independently interpreted by 2 observers. Positive percent agreement (PPA), negative percent agreement (NPA), and inter-reader agreement were calculated relative to CBM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The Anigen Rapid C.Brucella Ab assay demonstrated 90.0% PPA (95% CI, 55.5-99.8) and 100% NPA (95% CI, 91.1-100). The FASTest\u00ae BRUCELLA canis assay demonstrated 100% PPA (95% CI, 69.1-100) and 92.5% NPA (95% CI, 79.6-98.4). The Canine Brucella LPS Antibody Rapid Test demonstrated 0% PPA (95% CI, 0-30.9) and 97.5% NPA (95% CI, 86.8-99.9). Inter-reader agreement was almost perfect for all assays (\u03ba\u00a0=\u00a00.81-1.00).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and clinical importance<\/jats:title>\n                    <jats:p>The Anigen Rapid C.Brucella Ab and FASTest\u00ae BRUCELLA canis assays showed good agreement with the CBM assay and may be useful rule out tests in dogs with clinical signs compatible with brucellosis. In low prevalence settings, positive results require confirmatory testing, and false negatives remain possible.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jvimsj\/aalag073","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T15:34:02Z","timestamp":1777563242000},"source":"Crossref","is-referenced-by-count":0,"title":["Clinical performance of 3 commercially available point-of-care antibody tests for\n                    <i>Brucella canis<\/i>\n                    in dogs"],"prefix":"10.1093","volume":"40","author":[{"given":"Callum","family":"Atkins","sequence":"first","affiliation":[{"name":"Hospital for Small Animals, Royal (Dick) School of Veterinary Studies, University of Edinburgh , Roslin, Edinburgh, EH25 9RG ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan","family":"Madden","sequence":"additional","affiliation":[{"name":"Hospital for Small Animals, Royal (Dick) School of Veterinary Studies, University of Edinburgh , Roslin, Edinburgh, EH25 9RG ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shauni","family":"Emanuel","sequence":"additional","affiliation":[{"name":"Hospital for Small Animals, Royal (Dick) School of Veterinary Studies, University of Edinburgh , Roslin, Edinburgh, EH25 9RG ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1591-9891","authenticated-orcid":false,"given":"Ioannis","family":"Oikonomidis","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy, Physiology and Pathology, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Leahurst Campus , Liverpool, CH64 7TE ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Franklin-Guild","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University , Ithaca, NY 14853 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassandra","family":"Guarino","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University , Ithaca, NY 14853 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glynn","family":"Woods","sequence":"additional","affiliation":[{"name":"William R. 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