{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T17:08:52Z","timestamp":1778346532798,"version":"3.51.4"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Translational Renin\u2013Angiotensin\u2013Aldosterone Interest Group"},{"DOI":"10.13039\/100005595","name":"University of California","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005595","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Davis Center for Companion Animal Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The tissue renin\u2013angiotensin system (RAS) has not been characterized in cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>To quantify RAS enzyme activity and angiotensin peptide (AP) concentrations in myocardial and renal tissue obtained at necropsy from healthy cats and cats with hypertrophic cardiomyopathy (HCM).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>A convenience sample of 17 adult purpose-bred cats, euthanized under other study protocols, with echocardiographically normal hearts (n\u00a0=\u00a08) or American College of Veterinary Internal Medicine stage B1 (n\u00a0=\u00a06) or C (n\u00a0=\u00a03) HCM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Prospective study. Tissues were incubated with spiked angiotensin I (AngI) or II (AngII) under control and inhibitor conditions to assess relative enzyme contributions to AngII or angiotensin 1-7 (Ang1-7) formation. Freezing was delayed for 3\u00a0h in paired samples from 7 cats. Angiotensin peptides were also directly quantified in homogenized tissues.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Renin\u2013angiotensin system enzyme activity was present in necropsy tissues, which formed AngII and Ang1-7 when incubated with AP precursors. The median contribution of angiotensin-converting enzyme to AngII formation exceeded 85% in all tissues. The 90% confidence limits of the geometric mean of the ratio of the angiotensin production of paired samples met the equivalence requirement in 1\/13 experiments. The AP concentrations were quantifiable and did not differ between cats with echocardiographically normal hearts and cats with HCM (myocardial AngI P\u00a0=\u00a0.11 and AngII P\u00a0=\u00a0.37; kidney AngI P\u00a0=\u00a0.84, AngII P\u00a0=\u00a0.73, and Ang 1-7 P\u00a0=\u00a0.84).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and clinical importance<\/jats:title>\n                    <jats:p>Necropsy tissue RAS enzyme activity and some AP concentrations were quantifiable in this cohort. Renin\u2013angiotensin system enzyme activity changed during a short (3\u00a0h) postmortem period.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jvimsj\/aalag078","type":"journal-article","created":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T16:15:50Z","timestamp":1778343350000},"source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the myocardial and renal renin\u2013angiotensin system in normal cats and cats with hypertrophic cardiomyopathy"],"prefix":"10.1093","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2678-7276","authenticated-orcid":false,"given":"James E","family":"Wood","sequence":"first","affiliation":[{"name":"Department of Medicine and Epidemiology, Veterinary Medical Teaching Hospital, University of California, Davis , Davis, CA 95616 ,","place":["United States"]},{"name":"James E. 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