{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T18:30:16Z","timestamp":1786559416563,"version":"build-2736575974"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":42,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 113-2313-B-002-004"],"award-info":[{"award-number":["NSTC 113-2313-B-002-004"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006477","name":"National Taiwan University","doi-asserted-by":"publisher","award":["114\u00a0L893503"],"award-info":[{"award-number":["114\u00a0L893503"]}],"id":[{"id":"10.13039\/501100006477","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Early detection of tumors might prolong the survival of dogs. Kynurenine 3-monooxygenase (KMO) and Ki-67 are tumor biomarkers, but few studies have described the clinical value of KMO and Ki-67 in veterinary medicine.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>This study aims to compare the activity of KMO and the concentration of Ki-67 between healthy dogs and dogs with neoplasms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>A total of 162 dogs with different types of tumors were included. Forty-six tumor-free individuals were included as the control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This is a case\u2013control study. Dogs with neoplasms, confirmed through medical record review, were recruited. The primary outcomes were the plasma concentrations of KMO and Ki-67, along with their diagnostic efficacy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Expression levels of KMO were significantly higher in dogs with neoplasms (median, 2.02\u00a0ng\/mL; IQR, 1.03-3.65) than in tumor-free individuals (median, 0.69\u00a0ng\/mL; IQR, 0.23-1.18; P\u00a0&amp;lt;\u00a0.0001). The values of Ki-67 were higher in dogs with neoplasms (median, 4.28\u00a0ng\/mL; IQR, 3.75-4.84) than in the control group (median, 3.80\u00a0ng\/mL; IQR, 3.31-4.43; P\u00a0&amp;lt;\u00a0.05). No significant correlation was found between KMO and Ki-67 concentrations (R2\u00a0=\u00a00.003, P\u00a0=\u00a0.48) in the tested samples and 50.6% (82\/162) of dogs exhibited inconsistent results. The area under the ROC curve for KMO and Ki-67 were 0.837 and 0.644.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and clinical importance<\/jats:title>\n                    <jats:p>This study presents that KMO is an effective biomarker and Ki-67 is an adjunctive marker. While lacking standalone diagnostic utility, Ki-67 reflects systemic tumor biology and complements biomarkers such as KMO.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jvimsj\/aalag145","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T17:57:36Z","timestamp":1786557456000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of plasma kynurenine 3-monooxygenase and Ki-67 as screening biomarkers for neoplasia in dogs"],"prefix":"10.1093","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5277-0097","authenticated-orcid":false,"given":"Ka-Mei","family":"Sio","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University , Taipei 10617 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