{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:33:53Z","timestamp":1791556433677,"version":"4.3.4"},"reference-count":96,"publisher":"Oxford University Press (OUP)","issue":"14","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Isoprenoids constitute the largest class of plant natural products and have diverse biological functions including in plant growth and development. In potato (Solanum tuberosum), the regulatory mechanism underlying the biosynthesis of isoprenoids through the mevalonate pathway is unclear. We assessed the role of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) homologs in potato development and in the metabolic regulation of isoprenoid biosynthesis by generating transgenic lines with down-regulated expression (RNAi-hmgr) or overexpression (OE) of one (StHMGR1 or StHMGR3) or two genes, HMGR and farnesyl diphosphate synthase (FPS; StHMGR1\/StFPS1 or StHMGR3\/StFPS1). Levels of sterols, steroidal glycoalkaloids (SGAs), and plastidial isoprenoids were elevated in the OE-HMGR1, OE-HMGR1\/FPS1, and OE-HMGR3\/FPS1 lines, and these plants exhibited early flowering, increased stem height, increased biomass, and increased total tuber weight. However, OE-HMGR3 lines showed dwarfism and had the highest sterol amounts, but without an increase in SGA levels, supporting a rate-limiting role for HMGR3 in the accumulation of sterols. Potato RNAi-hmgr lines showed inhibited growth and reduced cytosolic isoprenoid levels. We also determined the relative importance of transcriptional control at regulatory points of isoprenoid precursor biosynthesis by assessing gene\u2013metabolite correlations. These findings provide novel insights into specific end-products of the sterol pathway and could be important for crop yield and bioenergy crops.<\/jats:p>","DOI":"10.1093\/jxb\/eraa185","type":"journal-article","created":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T11:11:47Z","timestamp":1586862707000},"page":"4109-4124","source":"Crossref","is-referenced-by-count":19,"title":["Altering potato isoprenoid metabolism increases biomass and induces early flowering"],"prefix":"10.1093","volume":"71","author":[{"family":"Moehninsi","sequence":"first","affiliation":[{"name":"Washington State University\/IAREC, Prosser, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Lange","sequence":"first","affiliation":[{"name":"Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B Markus","family":"Lange","sequence":"first","affiliation":[{"name":"Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duroy A","family":"Navarre","sequence":"first","affiliation":[{"name":"Washington State University\/IAREC, Prosser, WA, USA"},{"name":"USDA\/Agricultural Research Service, Prosser, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,4,16]]},"reference":[{"key":"2020070610443508500_CIT0001","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.1016\/j.cub.2019.02.018","article-title":"Source\u2013sink regulation is mediated by interaction of an FT homolog with a sweet protein in potato","volume":"29","author":"Abelenda","year":"2019","journal-title":"Current Biology"},{"key":"2020070610443508500_CIT0002","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1104\/pp.102.018788","article-title":"Reduction of cholesterol and glycoalkaloid levels in transgenic potato plants by overexpression of a type 1 sterol methyltransferase cDNA","volume":"131","author":"Arnqvist","year":"2003","journal-title":"Plant Physiology"},{"key":"2020070610443508500_CIT0003","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1007\/BF02534307","article-title":"Hydroxymethylglutaryl-CoA reductase, a key enzyme in phytosterol synthesis?","volume":"21","author":"Bach","year":"1986","journal-title":"Lipids"},{"key":"2020070610443508500_CIT0004","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1515\/znc-1982-1-209","article-title":"Mevinolin: a highly specific inhibitor of microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase of radish plants","volume":"37","author":"Bach","year":"1982","journal-title":"Zeitschrift fur Naturforschung. 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