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Although changes in light intensity and Mn status can interfere with the functionality of the photosynthetic apparatus, the interaction between these two factors and the underlying mechanisms remain largely unknown. Here, maize seedlings were grown hydroponically and exposed to two different light intensities under Mn-sufficient or -deficient conditions. No visual Mn deficiency symptoms appeared even though the foliar Mn concentration in the Mn-deficient treatments was reduced to 2 \u00b5g g\u20131. However, the maximum quantum yield efficiency of PSII and the net photosynthetic rate declined significantly, indicating latent Mn deficiency. The reduction in photosynthetic performance by Mn depletion was further aggravated when plants were exposed to high light intensity. Integrated transcriptomic and proteomic analyses showed that a considerable number of genes encoding proteins in the photosynthetic apparatus were only suppressed by a combination of Mn deficiency and high light, thus indicating interactions between changes in Mn nutritional status and light intensity. We conclude that high light intensity aggravates latent Mn deficiency in maize by interfering with the abundance of PSII proteins.<\/jats:p>","DOI":"10.1093\/jxb\/eraa366","type":"journal-article","created":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T11:29:41Z","timestamp":1596194981000},"page":"6116-6127","source":"Crossref","is-referenced-by-count":13,"title":["High light intensity aggravates latent manganese deficiency in maize"],"prefix":"10.1093","volume":"71","author":[{"given":"Lizhi","family":"Long","sequence":"first","affiliation":[{"name":"Key Lab of Plant-Soil Interaction, MOE, College Resources and Environmental Sciences, China Agricultural University, Beijing, China"},{"name":"Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej, Frederiksberg C, Denmark"},{"name":"Department of Agronomy, Zhejiang University, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pai R","family":"Pedas","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej, Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekka K","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej, Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waltraud X","family":"Schulze","sequence":"additional","affiliation":[{"name":"Institute for Physiology and Biotechnology of Plants, University of Hohenheim, Garbenstra\u00dfe, Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren","family":"Husted","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej, Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Zhejiang University, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2852-3298","authenticated-orcid":false,"given":"Jan K","family":"Schjoerring","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej, Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0233-6184","authenticated-orcid":false,"given":"Lixing","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Lab of Plant-Soil Interaction, MOE, College Resources and Environmental Sciences, China Agricultural University, Beijing, China"},{"name":"Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,8,1]]},"reference":[{"key":"2020100801344202600_CIT0001","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1006\/abbi.1997.0260","article-title":"NADP-malic enzyme from the C4 plant Flaveria bidentis: nucleotide substrate specificity","volume":"345","author":"Ashton","year":"1997","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"2020100801344202600_CIT0002","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1093\/jxb\/erz011","article-title":"C4-like photosynthesis and the effects of leaf senescence on C4-like physiology in Sesuvium sesuvioides (Aizoaceae)","volume":"70","author":"Bohley","year":"2019","journal-title":"Journal of Experimental Botany"},{"key":"2020100801344202600_CIT0003","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.jphotobiol.2011.01.025","article-title":"Auxiliary functions of the PsbO, PsbP and PsbQ proteins of higher plant photosystem II: a critical analysis","volume":"104","author":"Bricker","year":"2011","journal-title":"Journal of Photochemistry and Photobiology. 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